Genomic surveillance for antimicrobial resistance — a One Health perspective

Nature Reviews Genetics, Journal Year: 2023, Volume and Issue: 25(2), P. 142 - 157

Published: Sept. 25, 2023

Language: Английский

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Making sense of sentinels: wildlife as the one health bridge for environmental antimicrobial resistance surveillance

Journal of Applied Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract Antimicrobial resistance (AMR), arising from decades of imprudent anthropogenic use antimicrobials in healthcare and agriculture, is considered one the greatest One Health crises facing globally. pollutants released human-associated sources are intensifying evolution environment. Due to various ecological factors, wildlife interact with these polluted ecosystems, acquiring resistant bacteria genes. Although recognised reservoirs disseminators AMR environment, current surveillance systems still primarily focus on clinical agricultural settings, neglecting this environmental dimension. Wildlife can serve as valuable sentinels reflecting ecosystem health, effectiveness mitigation strategies. This review explores knowledge gaps surrounding factors influencing acquisition dissemination wildlife, highlights limitations policy instruments that do not sufficiently address component AMR. We discuss underutilised opportunity using sentinel species a holistic, Health-centred system. By better integrating into systematic policy, leveraging advances high-throughput technologies, we track predict evolution, assess impacts, understand complex dynamics transmission across ecosystems.

Language: Английский

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Genomic analysis of extended-spectrum beta-lactamase-producing E. coli from Czech diary calves and their caretakers

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 12, 2025

Introduction The increasing prevalence of antimicrobial resistance in livestock, particularly the dissemination extended-spectrum beta-lactamase-producing Escherichia coli , poses a significant zoonotic and public health risk. This study investigates genomic characteristics cefotaxime-resistant E. isolates from dairy calves across 23 Czech farms their caretakers. Materials methods Bacteriological cultivation on McConkey agar with cefotaxime was used for isolation, susceptibility to selected antibiotics determined by disc diffusion method, production beta-lactamases (ESBL) demonstrated double synergy test. PCR applied confirm presence genes encoding some beta-lactams quinolones carried plasmids. Using whole-genome sequencing, we evaluated genotypes, sequence types, serotypes, plasmid replicons, virulence genes. Results discussion Among 266 rectal samples obtained calves, 128 (48%) harbored . Whole-genome analysis revealed bla CTX-M 91% (116/128) isolates, -14 (44%) -1 (34%) being dominant variants. Other beta-lactamase gene TEM-1b found 40% (51/128) isolates. Notably, no cephamycin have been identified. plasmid-mediated quinolone (PMQR) qnrS1 present at 21% (27/128) colistin mcr-1 single ST2325 isolate. Sequence typing clonal diversity, 21 different STs detected among 68 sequenced ST10 most prevalent (27%), followed ST69 (12%), ST29 (7%) others. phylogenetic distribution showed predominance commensal groups A (54%) B1 (21%). common serotypes included O101:H9 (21%), O15:H18 H12, O70:H11 (7%). Analysis content complex 18 distinct replicon especially IncF, Col-type IncI1-type Cross-species transmission indicated detection strains shared between caretakers, notably ST10-O101:H9 ST34-O68:H30. high-risk clones mobile elements underscore urgent need stringent monitoring, stewardship, improved biosecurity measures livestock environments like increased caution personal hygiene animal handlers mitigate spread resistant animals humans.

Language: Английский

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Rabbits as a Reservoir of Multidrug-Resistant Escherichia coli: Clonal Lineages and Public Health Impact

Antibiotics, Journal Year: 2024, Volume and Issue: 13(4), P. 376 - 376

Published: April 20, 2024

Escherichia coli, including extended-spectrum β-lactamases (ESBL)-producing strains, poses a global health threat due to multidrug resistance, compromising food safety and environmental integrity. In industrial settings, rabbits raised for meat have the highest consumption of antimicrobial agents compared other food-producing animals. The European Union is facing challenges in rabbit farming as declines antibiotic-resistant strains E. coli cause enteric diseases. aim this study was investigate antibiotic resistance profile, genetic diversity, biofilm formation cefotaxime-resistant isolated from twenty farms Northern Portugal address effect pressing issue industry. Resistance critically antibiotics observed, with high levels several categories, such tetracycline, ampicillin, aztreonam, streptomycin. However, all isolates were susceptible cefoxitin imipenem. Multidrug common, showing tested. blaCTX-M variants (blaCTX-3G blaCTX-M9), followed by tetracycline genes, most frequent genes found. ST10 clones exhibiting significant various categories harboring different detected. ST457 ST2325 important sequence types their association ESBL-E. been widely distributed variety environments host species. evaluated showed capacity formation, which varied when they grouped number classes (i.e., seven antibiotics, six three/four/five antibiotics). One Health approach integrates efforts combat through interdisciplinary collaboration human, animal, health. Our findings are worrisome raise concerns. extensive usage emphasizes urgent need establish active surveillance systems.

Language: Английский

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Genomic insights into ESBL-producing Escherichia coli isolated from non-human primates in the Peruvian Amazon

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 10

Published: Jan. 16, 2024

Introduction Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are on the WHO priority pathogens list because they associated with high mortality, health-care burden, and antimicrobial resistance (AMR), a serious problem that threatens global public health should be addressed through One Health approach. Non-human primates (NHP) have risk of acquiring these antibiotic-resistant bacteria due to their close phylogenetic relationship humans increased anthropogenic activities in natural environments. This study aimed detect analyze genomes ESBL-producing Escherichia coli (ESBL-producing E. ) NHP from Peruvian Amazon. Materials methods We collected total 119 fecal samples semi-captive Saguinus labiatus , mystax Saimiri boliviensis captive Ateles chamek Cebus unicolor Lagothrix lagothricha Sapajus apella Loreto Ucayali regions, respectively. Subsequently, we isolated identified strains by microbiological methods, detected susceptibility tests following CLSI guidelines, analyzed using previously described genomic methods. Results 7.07% (7/99) strains: 5.45% (3/55) 9.09% (4/44) Ucayali, expressed ESBL phenotype. Genomic analysis revealed presence high-risk pandemic clones, such as ST10 ST117, carrying broad resistome relevant antibiotics, including three bla CTX-M variants: CTX-M-15 CTX-M-55 CTX-M-65 . Phylogenomic confirmed clonal relatedness lineages circulating at human-NHP interface. Additionally, two were EPEC ( eae ExPEC according virulence profiles, one more presented hypermucoviscous Discussion report detection seven factors regions Some closely related reported domestic animals, highlighting negative impact Amazonian wildlife. To our knowledge, this is first documentation Amazon, underscoring importance adopting approach AMR surveillance minimizing potential transmission

Language: Английский

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Whole Genome Sequencing Reveals High Genetic Diversity, Diverse Repertoire of Virulence-Associated Genes and Limited Antibiotic Resistance Genes among Commensal Escherichia coli from Food Animals in Uganda

Microorganisms, Journal Year: 2023, Volume and Issue: 11(8), P. 1868 - 1868

Published: July 25, 2023

Commensal Escherichia coli with broad repertoire of virulence and antimicrobial resistance (AMR) genes pose serious public health risks as reservoirs AMR virulence. This study undertook whole genome characterization commensal E. from food-producing animals in Uganda to investigate their variability (resistome virulome). We established that the had high genomic diversity 38 sequence types, 24 FimH 33 O-antigen serotypes randomly distributed within three phylogroups (A, B1, E). A greater proportion (≥93.65%) were resistant amoxicillin/clavulanate ampicillin antibiotics. The isolates AmpC beta-lactamase producers dominated by blaEC-15 (71.88%) tet(A) (20.31%) besides a diverse armory virulence-associated class exotoxin, adhesins, iron uptake, serine protease autotransporters which varied host species. Cattle found be major source carrying Shiga toxin genes, whereas swine was main colicin-like Usp gene. underscores importance livestock carrier large traits potential causing disease humans acquiring more genetic traits.

Language: Английский

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Rattus spp. as Reservoirs of Multidrug Resistance- and Biofilm-Forming Escherichia coli in Urban Community from Southern Thailand

Foodborne Pathogens and Disease, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Rats are rodents commonly found in Thailand that carry various zoonotic pathogens. Bacterial zoonosis can occur a shared environment between humans and rats, especially human communities agricultural areas.

Language: Английский

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Gram‐negative bacterial diversity and evidence of international clones of multidrug‐resistant strains in zoo animals

Integrative Zoology, Journal Year: 2023, Volume and Issue: 19(3), P. 417 - 423

Published: Nov. 20, 2023

Enterobacterales and Pseudomonas aeruginosa have been colonizing or infecting wild hosts antimicrobial-resistant strains are present in mammals birds. Furthermore, international high-risk clones of multidrug-resistant Escherichia coli identified the implications Gram-negative bacteria zoo animals discussed. The authors declare no conflicts interest. Please note: publisher is not responsible for content functionality any supporting information supplied by authors. Any queries (other than missing content) should be directed to corresponding author article.

Language: Английский

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Microorganism Diversity Found in Blatta orientalis L. (Blattodea: Blattidae) Cuticle and Gut Collected in Urban Environments

Insects, Journal Year: 2024, Volume and Issue: 15(11), P. 903 - 903

Published: Nov. 19, 2024

Pest cockroaches share urban habitats with us; their prevalence in areas prompts concerns regarding effect on human health, as synanthropic often host pathogenic microorganisms. Nonetheless, microbial associates these insects can also be related to biology, contributing physiological homeostasis and reproductive success. In this article, we present detail, for the first time, bacterial community associated oriental cockroach

Language: Английский

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Genome sequencing of Escherichia coli phage UFJF_EcSW4 reveals a novel lytic Kayfunavirus species

3 Biotech, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 15, 2024

Language: Английский

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