Genomic profiling of extended-spectrum β-lactamase-producing Escherichia coli from Pets in the United Arab Emirates: Unveiling colistin resistance mediated by mcr-1.1 and its probable transmission from chicken meat – A One Health perspective

Journal of Infection and Public Health, Journal Year: 2023, Volume and Issue: 16, P. 163 - 171

Published: Nov. 3, 2023

The United Arab Emirates (UAE) has witnessed rapid urbanization and a surge in pet ownership, sparking concerns about the possible transfer of antimicrobial resistance (AMR) from pets to humans environment. This study delves into whole-genome sequencing analysis ESBL-producing E. coli strains healthy cats dogs UAE, which exhibit multidrug (MDR). Additionally, it provides genomic exploration mobile colistin gene mcr-1.1, marking first instance its detection Middle Eastern pets.We investigate 17 UAE using WGS bioinformatics identify genes encoding virulence factors, assign diverse typing schemes isolates, scrutinize presence AMR genes. Furthermore, we characterized plasmid contigs housing mcr-1.1 conducted phylogenomic evaluate their relatedness previously identified isolates.Our unveiled variety factor-encoding within with fimH emerging as most prevalent. Regarding β-lactamase genes, blaCTX group 1 family predominated, CTX-M-15 found 52.9% (9/17) followed by CTX-M-55 29.4% (5/17). These isolates were categorized multiple sequence types (STs), epidemic ST131 being frequent. gene, linked resistance, was confirmed two isolates. belonged ST1011 displayed distinct profiles Phylogenomic revealed close connections between those chicken meat UAE.Our underscores MDR pets. identification mcr-1.1-carrying warrants urgency comprehensive surveillance highlights role companion animals epidemiology. findings underscore significance adopting One Health approach mitigate transmission risks effectively.

Language: Английский

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Characterization of Antimicrobial Resistance in Escherichia coli Isolated from Diarrheic and Healthy Weaned Pigs in Catalonia

Animals, Journal Year: 2024, Volume and Issue: 14(3), P. 487 - 487

Published: Feb. 1, 2024

Postweaning diarrhea (PWD) is a multifactorial concern in the swine industry that leads to high antibiotic consumption, usually without testing susceptibility, increasing risk of selection Escherichia coli-resistant strains. In this study, 251 E. coli strains isolated from fecal samples diarrheic (n = 148) and apparently healthy piglets 103) farms Catalonia were tested against their susceptibility fourteen different antimicrobials. The phenotypic antimicrobial resistance (AMR) revealed levels AMR, with 41.4% isolates presenting multidrug-resistant (MDR) profile. More specifically, class D (prudence) antimicrobials such as erythromycin (99.6%), amoxicillin (95.2%), streptomycin (91.6%), tetracycline (88.8%), lincospectin (64.5%), sulfamethoxazole/trimethoprim (60%) was very high, well C (caution) florfenicol (45%). A special observed for category B (restrict), like quinolones colistin, both presented rate resistance. Colistin use substantially reduced Spain, but still present weaned pigs, MIC90 4 μg/mL. This suggests reducing not enough eliminate AMR. Finally, it found suffering more commonly carriers MDR than ones (49.3% vs. 35%, p 0.031). Therefore, given rates most used antimicrobials, especially diseased new non-antibiotic-based approach should be implemented management PWD.

Language: Английский

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Genomic Evaluation of Multidrug-Resistant Extended-Spectrum β-Lactamase (ESBL)-Producing Escherichia coli from Irrigation Water and Fresh Produce in South Africa: A Cross-Sectional Analysis

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(32), P. 14421 - 14438

Published: Aug. 5, 2024

Escherichia coli, both commensal and pathogenic, can colonize plants persist in various environments. It indicates fecal contamination water food serves as a marker of antimicrobial resistance. In this context, 61 extended-spectrum β-lactamase (ESBL)-producing E. coli from irrigation fresh produce previous studies were characterized using whole genome sequencing (Illumina MiSeq). The Center for Genomic Epidemiology Galaxy platforms used to determine resistance genes, virulence plasmid typing, mobile genetic elements, multilocus sequence typing (MLST), pathogenicity prediction. total, 19 known MLST groups detected among the isolates. Phylogroup B1 (ST58) E (ST9583) most common types. six ST10 (serotype O101:H9) isolates carried spanning eight antibiotic classes. Overall, 95.1% genes three or more blaCTX-M-1, blaCTX-M-14, blaCTX-M-15 ESBL associated with all showed >90% predicted probability being human pathogen. This study provided novel genomic information on environmental multidrug-resistant ESBL-producing water, highlighting environment reservoir strains emphasizing need ongoing pathogen surveillance within One Health context.

Language: Английский

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Genomic Epidemiology of Multidrug-Resistant Escherichia coli and Klebsiella pneumoniae in Kenya, Uganda, and Jordan

Emerging infectious diseases, Journal Year: 2024, Volume and Issue: 30(14)

Published: Oct. 1, 2024

Surveillance of antimicrobial resistance in Kenya, Uganda, and Jordan identified multidrug-resistant high-risk bacterial clones: Escherichia coli sequence types 131, 1193, 69, 167, 10, 648, 410, 405 Klebsiella pneumoniae 14, 147, 307, 258. Clones emerging those countries exhibited high mechanism diversity, highlighting a serious threat for multidrug resistance.

Language: Английский

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Genomic heterogeneity of Multidrug Resistance E.coli recovered from diarrheagenic children under five years from Mukuru Informal Settlement, Nairobi, Kenya

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 29, 2024

Abstract Introduction High genomic plasticity within E. coli enables it to acquire and accumulate genetic material through horizontal gene transfer like mobile elements. In this study, we sought investigate the virulence genes, phylogroups, antibiotic resistance genes( ARGs), plasmid replicons, MLST, cgMLST of multidrug-resistant E.coli recovered from diarrheagenic children under five years Mukuru Informal Settlement, Nairobi Kenya. Methods A total 39 MDR strains had their DNA extracted, Whole Genome Sequencing was done using Illumina HiSeq 2500 platform. Twenty-six assemblies were analyzed web-based bioinformatics tools available at Centre for Genomic Epidemiology (TDU) EnteroBase. Results The isolates fell into 4 main where 10/26(38.5%) belonged B2 phylogroup, 4/26 ( 15.4%) D, 3/26( 11.5%) A, 1/26( 3.8%) B1, while 8/26 30.8%) not determined. FimH30 predominantly found in most frequent phylogroup Sequence Type(ST) 131. 40 diverse genes detected among isolates. 13 different STs isolated genomes, which included ST 131, 3036, 38, 10, 12569, 15271, 2076, 311, 3572, 394, 453, 46 1722. Only two 2/26, 7.7%) Municipal City Council MCC) clinic genetically related. Additionally, abundant replicon identified IncF family, IncFII(pRSB107) particular, followed by Col family. Of 26 isolates, 15 least one nonsynonymous mutation housekeeping gyrA (p.S83L), (p.D87N), parC (p.S80I), (p.E84V), parC(p.S57T), parE(p.I529L), associated with fluoroquinolones. Conclusion study highlighted first ST46 harbor NDM5 encoded Col(BS512), IncFII(pRSB107), IncFIB(AP001918) replicons We further demonstrated diversity diarrhea an endemic setting

Language: Английский

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Characterisation and Bacteriophage Sensitivity of Enterococci Isolated from Traditional Fermented Dairy Product

European Journal of Nutrition & Food Safety, Journal Year: 2024, Volume and Issue: 16(7), P. 211 - 221

Published: June 21, 2024

Aims: The study aimed to detect and classify Enterococci isolates from Thayir, a traditional fermented dairy product. Study Design: A statistically significant number of Thayir samples were collected across the area isolate characterise Enterococci. Place Duration Study: took place in Wayanad District Kerala, India 2022-2023. Methodology: aseptically bacteriologically cultured enterococci. obtained tested for ability form biofilms hydrolyse gelatin. antibiotic sensitivity pattern was studied using disk diffusion assay. simple Kmeans clustering algorithm applied group isolates. Phage-sensitive resistant identified among safer Results: Eight obtained. None hydrolysed gelatin, but four produced biofilm. Antibiogram revealed increased nitrofurantoin, most sensitive antimicrobials. Clustering analysis set three isolates, which one phage EFΦ91. Conclusion: presence Enterococcus fecalis faecium thayir samples. Analyses that diverse continued monitoring antimicrobial resistance against food is necessary.

Language: Английский

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