p38- and ERK-MAPK Signalling Modulate Developmental Neurotoxicity of Nickel and Vanadium in the Caenorhabditis elegans Model

Kinases and Phosphatases, Journal Year: 2024, Volume and Issue: 2(1), P. 28 - 42

Published: Jan. 4, 2024

Nickel (Ni) and vanadium (V) are characteristic heavy metal constituents of many crude oil blends in Sub-Saharan Africa, we have previously demonstrated their neurotoxic impact. However, molecular mechanisms driving Ni V neurotoxicity still being elucidated. The p38- ERKs-MAPK pathways, which mostly known for involvement human immune inflammatory signalling, been shown to influence an array neurodevelopmental processes. In the present study, attempt elucidate role ERK-MAPK after early life exposures using Caenorhabditis elegans model. Synchronized larvae stage-1 (L1) worms were treated with varying concentrations singly or combination 1 h. Our results show induces lethality C. even at very low concentrations, while much higher required induce lethality. Furthermore, identified that loss-of-function pmk-1 pmk-3, both homologous p38-α (MAPK14), is differentially affected by exposures. Also, all exposure scenarios triggered significant developmental delays wild-type mutant strains. We also see increased mitochondrial-derived reactive oxygen species following differential responses Additionally, observed alterations dopamine serotonin levels exposures, particularly strain. conclusion, lethality, delays, ROS worms, requiring a concentration. Further, suggest signalling pathways may modulate toxicity, potentially affecting mitochondrial health, bioavailability, neurotransmitter levels.

Language: Английский

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Selenium metabolism and selenoproteins function in brain and encephalopathy

Science China Life Sciences, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Language: Английский

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Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis

Toxics, Journal Year: 2023, Volume and Issue: 11(9), P. 753 - 753

Published: Sept. 5, 2023

Environmental pollutants, particularly toxic trace metals with neurotoxic potential, have been related to the genesis of autism. One these that stands out, in particular, is lead (Pb). We conducted an in-depth systematic review and meta-analysis peer-reviewed studies on Pb levels biological materials retrieved from autistic children (cases) neurotypical (controls) this work. A was after careful selection published according established criteria gain a broad insight into higher or lower cases controls, findings were then strengthened by meta-analysis. The included 17 (hair), 13 (whole blood), 8 (urine). overall number controls/cases 869/915 670/755 344/373 This showed significantly all three types material than suggesting body burden children. Thus, environmental exposure could be Since no level can considered safe, data study undoubtedly point importance regularly monitoring

Language: Английский

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Neurogenic Effects of Inorganic Arsenic and Cdk5 Knockdown in Zebrafish Embryos: A Perspective on Modeling Autism

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3459 - 3459

Published: March 19, 2024

The exact mechanisms of the development autism, a multifactorial neurological disorder, are not clear. pathophysiology autism is complex, and investigations at cellular molecular levels ongoing to provide clarity. Mutations in specific genes have been identified as risk factors for autism. role heavy metals pathogenesis subject many studies remains debatable. Although no neuronal phenotypes linked autistic symptoms, overproduction reduction neurons implicated. A growing literature on generating genetic non-genetic models aims help with understanding mechanistic that can explain complexity disorder. Both methods zebrafish used model For several human genes, validated mutant generated. There evidence indicating potential link between inorganic arsenic exposure. We previously shown induces supernumerary spinal motor via Sonic hedgehog (Shh) signaling pathway, Cdk5 knockdown causes an cranial zebrafish. Here, this review, we perspective what these findings neurogenic mean terms dysregulated pathways neuron their applicability underpinnings

Language: Английский

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Determination of Aluminium by Potentiometry Using Carbon Paste Ion Selective Electrode Based on N,N'-bis(salicylidene)-1,3-Propanediamine Ligand

Journal of Analytical Chemistry, Journal Year: 2022, Volume and Issue: 77(8), P. 1057 - 1061

Published: Aug. 1, 2022

Language: Английский

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Reference Values on Children’s Hair for 28 Elements (Heavy Metals and Essential Elements) Based on a Pilot Study in a Representative Non-Contaminated Local Area

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(9), P. 8127 - 8127

Published: May 1, 2023

Studies have been published, and laboratories offer services of measuring elements in hair as biomarkers environmental exposure and/or control essential (trace or macro). These reported values can only sense if compared with adopted reference values. In this work, we propose provisional based on a pilot child population. The concentrations 28 were measured children's samples. An observational, descriptive, cross-sectional study was conducted typical population the Mediterranean region void excessive pollution problems to analyze 419 samples children aged 3-12 years. Children selected by simple random method from eight primary education schools different municipal districts, which included urban, rural industrial areas. Samples around 100 mg washed acid digested an optimized procedure. All measures performed using ICP-MS Sc, Y Re internal standards. statistical analysis two approaches: (a) considering all data (b) without outliers (second-order atypical data) compare them other published studies. distribution curves studied asymmetric did not fit theoretical normality distributions. Therefore, percentiles more appropriate. most elements, slight differences observed sex age, justify proposing separate ranges. From results study, are proposed following criteria: application table built removing outlier after detailed case-by-case, profile each element. Although sample limited area, it carefully be representative general non-contaminated With limitation, might useful for researchers physicians until wider geographical is available large number elements.

Language: Английский

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Chemical Elements in Hair and Their Association with Autism Spectrum Disorder: A Comprehensive Systematic Review

Pollutants, Journal Year: 2023, Volume and Issue: 3(4), P. 587 - 602

Published: Dec. 5, 2023

Background: Autism Spectrum Disorder (ASD) is a complex neurodevelopmental condition with increasing prevalence. This review aims to systematically investigate the relationship between levels of toxic and trace elements in hair occurrence ASD. Methods: Original articles reporting element were included. A comprehensive search was conducted databases such as Medline (via PubMed), Scopus, Web Science, Google Scholar, covering publications from 1 January 2000, 2 2023. Keywords including “hair analysis”, “trace elements”, “autism”, utilized search. Results: direct comparison data not possible due variety methodologies observed available studies, variations sample sizes analytical procedures. Conclusions: Exposure elements, notably Mercury (Hg) Lead (Pb), which act neurotoxicants, found be associated pathogenesis Furthermore, significant correlation identified Zinc (Zn) Copper (Cu) ratio

Language: Английский

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Transcriptome Study in Sicilian Patients with Autism Spectrum Disorder

Biomedicines, Journal Year: 2024, Volume and Issue: 12(7), P. 1402 - 1402

Published: June 25, 2024

ASD is a complex condition primarily rooted in genetics, although influenced by environmental, prenatal, and perinatal risk factors, ultimately leading to genetic epigenetic alterations. These mechanisms may manifest as inflammatory, oxidative stress, hypoxic, or ischemic damage. To elucidate potential variances gene expression ASD, transcriptome analysis of peripheral blood mononuclear cells was conducted via RNA-seq on 12 patients 13 healthy controls, all Sicilian ancestry minimize environmental confounds. A total 733 different statistically significant genes were identified between the two cohorts. Gene Set Enrichment Analysis (GSEA) Ontology (GO) terms employed explore pathways differentially expressed mRNAs. GSEA revealed GO strongly associated with namely Biological Process term "Response Oxygen-Containing Compound". Additionally, Cellular Component pathway "Mitochondrion" stood out among other pathways, predominantly affiliated this specific pathway, implicating involvement mitochondrial functions ASD. Among genes,

Language: Английский

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Shared Developmental Neuropathological Traits Between Autism and Environmental Lead Exposures: Insights into Convergent Sulfur-Dependent Neurobiological Mechanisms

Springer eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 227 - 250

Published: Jan. 1, 2023

Language: Английский

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Inductively coupled plasma mass spectrometry based urine metallome to construct clinical decision models for autism spectrum disorder

Metallomics, Journal Year: 2022, Volume and Issue: 14(12)

Published: Jan. 1, 2022

Abstract Background The global prevalence of autism spectrum disorder (ASD) is on the rise, and high levels exposure to toxic heavy metals may be associated with this increase. Urine analysis a noninvasive method for investigating accumulation excretion metals. aim study was identify ASD-associated urinary metal markers. Methods Overall, 70 children ASD 71 typical development (TD) were enrolled in retrospective case–control study. In metallomics investigation, inductively coupled plasma mass spectrometry performed obtain urine profile 27 Results Children could distinguished from TD based profile, showing an increased Shannon diversity. A metallome-wide association used seven ASD-related urine, cobalt, aluminum, selenium, lithium significantly higher, manganese, mercury, titanium lower than TD. least absolute shrinkage selection operator (LASSO) machine learning rank terms their effect ASD. On basis these metals, we constructed LASSO regression model classification found area under receiver operating characteristic curve 0.913. We also clinical prediction that different would useful risk. Conclusions findings suggest altered concentrations important risk factor ASD, recommend further exploration mechanisms treatment measures such alterations.

Language: Английский

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