The sulfated peptide CLEL6 is a negative regulator of anthocyanin biosynthesis inArabidopsis thaliana DOI Creative Commons
Eric Bühler, Elisa Fahrbach, Andreas Schaller

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 25, 2022

Abstract Post-translationally modified peptides are now recognized as important regulators of plant stress responses. Here we identified the small sulfated CLE-LIKE6 (CLEL6) peptide a negative regulator stress-induced anthocyanin biosynthesis. The expression CLEL6 and its effect on biosynthesis were strongly down-regulated by light. function depends proteolytic processing precursor subtilisin-like serine proteinase 6.1 (SBT6.1), tyrosine sulfation tyrosylprotein sulfotransferase (TPST). Loss mutants either sbt6.1 or tpst showed significantly higher accumulation upon light stress. overaccumulation phenotype was suppressed application mature CLEL6. Further confirming role an inhibitor biosynthesis, overexpression external inhibited genes involved in etiolated light-stressed seedlings. Small post-translationally known to be perceived leucine-rich-repeat receptor like kinases. Through genetic approach, using ROOT MERISTEM GROWTH FACTOR 1 INSENSITIVE (RGI) quintuple mutant, could show essential RGI family signaling. Our data indicate that inhibits through receptors dark-grown seedlings, this inhibition is released when transition One sentence summary formation SBT6.1, post-translational modification TPST, perception receptors.

Language: Английский

Subtilase-mediated biogenesis of the expanded family of SERINE RICH ENDOGENOUS PEPTIDES DOI
Huanjie Yang,

Xeniya Kim,

Jan Sklenář

et al.

Nature Plants, Journal Year: 2023, Volume and Issue: 9(12), P. 2085 - 2094

Published: Dec. 4, 2023

Language: Английский

Citations

27
Arabidopsis SBT5.2 and SBT1.7 subtilases mediate C-terminal cleavage of flg22 epitope from bacterial flagellin DOI Creative Commons
Sayaka Matsui,

Saki Noda,

Keiko Kuwata

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 4, 2024

Abstract Plants initiate specific defense responses by recognizing conserved epitope peptides within the flagellin proteins derived from bacteria. Proteolytic cleavage of plant apoplastic proteases is thought to be crucial for perception receptor. However, identity involved in this process remains unknown. Here, we establish an efficient identification system target Arabidopsis fluid; method employs native two-dimensional electrophoresis followed in-gel proteolytic assay using a fluorescence-quenching peptide substrate. We designed substrate specifically detect activity at C-terminus flg22 and identified two subtilases, SBT5.2 SBT1.7, as responsible C-terminal flg22. In fluid mutant plants deficient these proteases, observe decrease domain flagellin, leading efficiency liberation. Consequently, defensive reactive oxygen species (ROS) production delayed sbt5.2 sbt1.7 double-mutant leaf disks compared wild type following exposure.

Language: Английский

Citations

13
Small peptides: novel targets for modulating plant–rhizosphere microbe interactions DOI
Weiyi Tan, Hai Nian, Lam-Son Phan Trân

et al.

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(11), P. 1072 - 1083

Published: April 25, 2024

Language: Английский

Citations

10
Proteolysis in plant immunity DOI
Yanan Liu, E. Jackson, Xueru Liu

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3099 - 3115

Published: May 9, 2024

Compared with transcription and translation, protein degradation machineries can act faster be targeted to different subcellular compartments, enabling immediate regulation of signaling events. It is therefore not surprising that proteolysis has been used extensively control homeostasis key regulators in biological processes pathways. Over the past decades, numerous studies have shown proteolysis, where proteins are broken down peptides or amino acids through ubiquitin-mediated systems proteases, a regulatory mechanism plant immunity output. Here, we briefly summarize roles various proteases play during defence activation, focusing on recent findings. We also update latest progress modulating by targeting membrane-localized pattern recognition receptors, intracellular nucleotide-binding domain leucine-rich repeat downstream components. Additionally, highlight showcasing importance maintaining broad-spectrum resistance without obvious yield reduction, opening new directions for engineering elite crops resistant wide range pathogens high yield.

Language: Английский

Citations

9
The lowdown on breakdown: Open questions in plant proteolysis DOI Creative Commons
Nancy A. Eckardt, Tamar Avin‐Wittenberg, Diane C. Bassham

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 2931 - 2975

Published: July 9, 2024

Abstract Proteolysis, including post-translational proteolytic processing as well protein degradation and amino acid recycling, is an essential component of the growth development living organisms. In this article, experts in plant proteolysis pose discuss compelling open questions their areas research. Topics covered include role cell cycle, DNA damage response, mitochondrial function, generation N-terminal signals (degrons) that mark many proteins for (N-terminal acetylation, Arg/N-degron pathway, chloroplast N-degron pathway), developmental metabolic signaling (photomorphogenesis, abscisic strigolactone signaling, sugar metabolism, postharvest regulation), responses to environmental (endoplasmic-reticulum-associated degradation, chloroplast-associated drought tolerance, growth-defense trade-off), functional diversification peptidases. We hope these thought-provoking discussions help stimulate further

Language: Английский

Citations

9
Detection of the Arabidopsis Proteome and Its Post-translational Modifications and the Nature of the Unobserved (Dark) Proteome in PeptideAtlas DOI
Klaas J. van Wijk, Mark Leppert, Zhi Sun

et al.

Journal of Proteome Research, Journal Year: 2023, Volume and Issue: 23(1), P. 185 - 214

Published: Nov. 21, 2023

This study describes a new release of the Arabidopsis thaliana PeptideAtlas proteomics resource (build 2023–10) providing protein sequence coverage, matched mass spectrometry (MS) spectra, selected post-translational modifications (PTMs), and metadata. 70 million MS/MS spectra were to Araport11 annotation, identifying ∼0.6 unique peptides 18,267 proteins at highest confidence level 3396 lower proteins, together representing 78.6% predicted proteome. Additional identified not in should be considered for next genome annotation. 5198 phosphorylated 668 ubiquitinated 3050 N-terminally acetylated 864 lysine-acetylated mapped their PTM sites. MS support was lacking 21.4% (5896 proteins) proteome: "dark" dark proteome is highly enriched E3 ligases, transcription factors, certain (e.g., CLE, IDA, PSY) but other THIONIN, CAP) signaling families. A machine learning model trained on RNA expression data properties predicts probability that will detected. The aids discovery with short half-life SIG1,3 ERF-VII TFs) developing strategies identify missing proteins. linked TAIR, tracks JBrowse, several community resources.

Language: Английский

Citations

15
Small Peptides: Orchestrators of Plant Growth and Developmental Processes DOI Open Access

Shuaiqi Lu,

Fei Xiao

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7627 - 7627

Published: July 11, 2024

Small peptides (SPs), ranging from 5 to 100 amino acids, play integral roles in plants due their diverse functions. Despite low abundance and small molecular weight, SPs intricately regulate critical aspects of plant life, including cell division, growth, differentiation, flowering, fruiting, maturation, stress responses. As vital mediators intercellular signaling, have garnered significant attention biology research. This comprehensive review delves into SPs’ structure, classification, identification, providing a detailed understanding significance. Additionally, we summarize recent findings on the biological functions signaling pathways prominent that growth development. also offers perspective future research directions peptide pathways.

Language: Английский

Citations

6
Characterization of Transcriptome Dynamics during Early Fruit Development in Olive (Olea europaea L.) DOI Open Access

Maria C. Camarero,

Beatriz Briegas, Jorge Corbacho

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 961 - 961

Published: Jan. 4, 2023

In the olive (Olea europaea L.), an economically leading oil crop worldwide, fruit size and yield are determined by early stages of development. However, few detailed analyses this stage development available. This study offers extensive characterization various processes involved in growth (cell division, cell cycle regulation, expansion). For this, cytological, hormonal, transcriptional changes characterizing phases were analyzed cv. ‘Picual’. First, surface area mitotic activity (by flow cytometry) cells investigated during development, from 0 to 42 days post-anthesis (DPA). The results demonstrate that division phase extends up 21 DPA, which maximal proportion 4C fruits was reached at 14 indicating intensive activated time. Subsequently, expansion lasted as long 3 weeks more before endocarp lignification. Finally, molecular mechanisms controlling analyzing transcriptome flowers anthesis (fruit set) well DPA phase) 28 phase). Sequential induction regulating genes is associated with upregulation wall remodeling ion fluxes, a shift plant hormone metabolism signaling occurs together subtilisin-like protease proteins transcription factors potentially signaling. gene expression profile, hormonal regulators, new insights for understanding regulate expansion, ultimately size.

Language: Английский

Citations

12
The peptide hormone Pj CLE1 stimulates haustorium formation in the parasitic plant Phtheirospermum japonicum DOI Creative Commons
Anne Greifenhagen, Hannes Ruwe,

Victoria Zimmer

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(42)

Published: Oct. 9, 2024

Phtheirospermum japonicum is a hemiparasitic plant of the Orobanchaceae, largest family parasitic plants. It extracts water and nutrients from other plants through haustoria along its roots. Haustoriogenesis, formation haustoria, initiated by host-derived haustorium-inducing factors (HIFs). The first step in haustoriogenesis development parasitically inactive protohaustoria. Here, we report that an endogenous peptide hormone, CLAVATA3/Embryo Surrounding Region 1 ( Pj CLE1), sufficient to induce protohaustorium formation. CLE1 hyperactivated HIF-responses caused prolific protohaustoria PjCLE1 expression activation subtilisin-type protease SBT1.2.3 occur fully developed, mature suggesting acts as internal signal produced stimulate additional for effective extraction resources hosts. similar sequence CLEs regulating nodulation legumes part regulatory system

Language: Английский

Citations

5
Extracellular plant subtilases dampen cold-shock peptide elicitor levels DOI Creative Commons
Changlong Chen, Pierre Buscaill, Nattapong Sanguankiattichai

et al.

Nature Plants, Journal Year: 2024, Volume and Issue: 10(11), P. 1749 - 1760

Published: Oct. 11, 2024

Abstract Recognizing pathogen-associated molecular patterns on the cell surface is crucial for plant immunity. The proteinaceous nature of many these suggests that secreted proteases play important roles in their formation and stability. Here we demonstrate apoplastic subtilase SBT5.2a inactivates immunogenicity cold-shock proteins (CSPs) bacterial pathogen Pseudomonas syringae by cleaving within immunogenic csp22 epitope. Consequently, mutant plants lacking activity retain higher levels csp22, leading to enhanced immune responses reduced growth. SBT5.2 sensitivity influenced sequence variation surrounding cleavage site probably extends CSPs from other species. These findings suggest variations stability among pathogens are a factor plant–bacteria interactions exploit avoid pattern recognition.

Language: Английский

Citations

4