Transposon-triggered epigenetic chromatin dynamics modulate EFR-related pathogen response DOI Creative Commons
Regina Mencia, A. Arce,

Candela Houriet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 9, 2023

Summary Infectious diseases drive the evolution of wild plants and impact yield in crop plants. Like animals, can sense biotic threats via conserved pathogen-associated patterns (PAMPs). Since an overly robust immune response harm plants, understanding mechanisms for tuning defense responses to appropriate level is vital as we endeavor develop pathogen-resistant crops. In this paper, studied Arabidopsis pattern recognition receptor (PRR) EFR, which senses bacterial EF-Tu. An inverted-repeat transposon ( Ea-IR ) between EFR neighboring XI-k locus controls local chromatin organization, promoting formation a repressive loop. Upon pathogen infection, landscape around Xl-k dynamically changes allow increased transcription. Chromatin opening facilitates passage RNA polymerase II across gene termination site, leading longer transcript that includes sequences. Dicer-like (DCL) enzymes process into small RNAs (sRNAs), reset topology state, attenuating, turn, response, reminiscent attenuation signaling other systems. From evolutionary point view, found natural accessions missing have constitutive " -open" configuration correlates with higher basal levels background resistance pathogens. Collectively, our study offers evidence scenario transposon, expression interact fine-tune responses, both during course infection evolution. Similar gene-associated IRs crops could provide valuable non-coding targets genome editing or assisted plant breeding programs.

Language: Английский

Role of plant secondary metabolites in defence and transcriptional regulation in response to biotic stress DOI Creative Commons

Anjali,

Sumit Kumar,

Tulasi Korra

et al.

Plant Stress, Journal Year: 2023, Volume and Issue: 8, P. 100154 - 100154

Published: April 3, 2023

Secondary metabolites in plants have been recognized as a novel basis of potential bio-pesticides, paving the way for their use sustainable agriculture. Plant secondary pivotal roles plant-pathogen interactions. Some important are terpenoids, flavanols, flavones, etc., stress-inducible phytochemicals playing an role plant immune response development. Pathogen enters into host cell, multiply and utilise biological mechanism plants, causing hazard to world food assembly. Under stressed circumstances, evolve powerful intricate system growth defensive action. On other hand, transcription factors (TFs) assist counter adverse environments by acting mediators stress signal regulating stress-responsive gene expression. The transcriptional post-transcriptional manipulation is capable aid molecular breeding genetic modification meant improved metabolite synthesis. Although presence numerous has established life, very slight known about interaction with pathogens specific mechanisms involved leading immunity. Chemical pesticides wreaking havoc on our environment. As consequence, environmental-friendly alternatives disease management, like plant-based metabolites, should be explored. In this appraisal, we reviewed relation pathogens, contribution innate immunity, action, regulation TFs combating infections eco-friendly approach.

Language: Английский

Citations

189
Pathogen perception and signaling in plant immunity DOI Creative Commons
Peter N. Dodds, Jian Chen, Megan A. Outram

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(5), P. 1465 - 1481

Published: Jan. 23, 2024

Abstract Plant diseases are a constant and serious threat to agriculture ecological biodiversity. Plants possess sophisticated innate immunity system capable of detecting responding pathogen infection prevent disease. Our understanding this has grown enormously over the past century. Early genetic descriptions plant disease resistance virulence were embodied in gene-for-gene hypothesis, while physiological studies identified pathogen-derived elicitors that could trigger defense responses cells tissues. Molecular these phenomena have now coalesced into an integrated model involving cell surface intracellular detection specific molecules proteins culminating induction various cellular responses. Extracellular receptors engage distinct signaling processes but converge on many similar outputs with substantial evidence for integration pathways interdependent networks controlling outcomes. Many molecular details recognition known, providing opportunities bioengineering enhance protection from Here we provide overview current main principles immunity, emphasis key scientific milestones leading insights.

Language: Английский

Citations

32
Plant disease resistance outputs regulated by AP2/ERF transcription factor family DOI Creative Commons

Ning Ma,

Ping Sun, Zhaoyang Li

et al.

Stress Biology, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 2, 2024

Abstract Plants have evolved a complex and elaborate signaling network to respond appropriately the pathogen invasion by regulating expression of defensive genes through certain transcription factors. The APETALA2/ethylene response factor (AP2/ERF) family members been determined as key regulators in growth, development, stress responses plants. Moreover, growing body evidence has demonstrated critical roles AP2/ERFs plant disease resistance. In this review, we describe recent advances for function defense against microbial pathogens. We summarize that are involved resistance acting downstream mitogen activated protein kinase (MAPK) cascades, associated with hormonal pathways, biosynthesis secondary metabolites, formation physical barriers an MAPK-dependent or -independent manner. present review provides multidimensional perspective on functions resistance, which will facilitate understanding future investigation immunity.

Language: Английский

Citations

15
Immune signaling: receptor-like proteins make the difference DOI

Wen R.H. Huang,

M.H.A.J. Joosten

Trends in Plant Science, Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

Language: Английский

Citations

13
Unlocking Nature's Defense: Plant Pattern Recognition Receptors as Guardians Against Pathogenic Threats DOI Creative Commons

Chao Zhang,

Yingpeng Xie, Ping He

et al.

Molecular Plant-Microbe Interactions, Journal Year: 2024, Volume and Issue: 37(2), P. 73 - 83

Published: Feb. 1, 2024

Embedded in the plasma membrane of plant cells, receptor kinases (RKs) and proteins (RPs) act as key sentinels, responsible for detecting potential pathogenic invaders. These were originally characterized more than three decades ago disease resistance (R) proteins, a concept that was formulated based on Harold Flor's gene-for-gene theory. This theory implies genetic interaction between specific R corresponding effectors, eliciting effector-triggered immunity (ETI). Over years, extensive research has unraveled their intricate roles pathogen sensing immune response modulation. RKs RPs recognize molecular patterns from microbes well dangers cells initiating pattern-triggered (PTI) danger-triggered (DTI), which have connections with ETI. Moreover, these are involved maintaining homeostasis preventing autoimmunity. review showcases seminal studies discovering discusses recent advances understanding functions signals cell integrity autoimmunity, ultimately contributing to robust balanced defense response. [Formula: see text] The author(s) dedicated work public domain under Creative Commons CC0 “No Rights Reserved” license by waiving all his or her rights worldwide copyright law, including related neighboring rights, extent allowed 2024.

Language: Английский

Citations

10
EDS1 complexes are not required for PRR responses and execute TNL‐ETI from the nucleus in Nicotiana benthamiana DOI Creative Commons
Josua Zönnchen, Johannes Gantner, Dmitry Lapin

et al.

New Phytologist, Journal Year: 2022, Volume and Issue: 236(6), P. 2249 - 2264

Published: Sept. 24, 2022

Heterodimeric complexes incorporating the lipase-like proteins EDS1 with PAD4 or SAG101 are central hubs in plant innate immunity. functions encompass signal relay from TIR domain-containing intracellular NLR-type immune receptors (TNLs) towards RPW8-type helper NLRs (RNLs) and, Arabidopsis thaliana, bolstering of signaling and resistance mediated by cell-surface pattern recognition (PRRs). Increasing evidence points to activation small molecule binding. We used CRISPR/Cas-generated mutant lines agroinfiltration-based complementation assays interrogate Nicotiana benthamiana. did not detect impaired PRR N. benthamiana deficient RNLs. Intriguingly, monitoring SlEDS1-NbEDS1 benthamiana, mutations within SlEDS1 catalytic triad could abolish enhance TNL Furthermore, nuclear accumulation was sufficient for (Roq1) Reinforcing might be a derived function TNL/EDS1 sector. Although Solanaceae functionally depends on residues some contexts, our data do support binding TNL-derived environment. Whether how activity connects membrane pore-forming RNLs remains unknown.

Language: Английский

Citations

27
Advances in Biological Control and Resistance Genes of Brassicaceae Clubroot Disease-The Study Case of China DOI Open Access
Chaoying Zhang, Chunyu Du,

Yuwei Li

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(1), P. 785 - 785

Published: Jan. 2, 2023

Clubroot disease is a soil-borne caused by Plasmodiophora brassicae. It occurs in cruciferous crops exclusively, and causes serious damage to the economic value of worldwide. Although different measures have been taken prevent spread clubroot disease, most fundamental effective way explore use disease-resistance genes breed resistant varieties. However, resistance level plant hosts influenced both environment pathogen race. In this work, we described terms discovery current distribution, life cycle, race identification systems; particular, summarized recent progress on control methods breeding practices for cultivars. With knowledge these identified loci R genes, discussed feasible strategies future.

Language: Английский

Citations

14
From molecule to cell: the expanding frontiers of plant immunity DOI
Lei Li, J. Liu, Jian‐Min Zhou

et al.

Journal of genetics and genomics/Journal of Genetics and Genomics, Journal Year: 2024, Volume and Issue: 51(7), P. 680 - 690

Published: Feb. 26, 2024

Language: Английский

Citations

5
Berberine bridge enzyme–like oxidases of cellodextrins and mixed-linked β-glucans control seed coat formation DOI
Sara Costantini, Manuel Benedetti, Daniela Pontiggia

et al.

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 194(1), P. 296 - 313

Published: Aug. 17, 2023

Plants have evolved various resistance mechanisms to cope with biotic stresses that threaten their survival. The BBE23 member (At5g44360/BBE23) of the Arabidopsis berberine bridge enzyme-like (BBE-l) protein family (Arabidopsis thaliana) has been characterized in this paper parallel closely related and previously described CELLOX (At4g20860/BBE22). In addition cellodextrins, both enzymes, renamed here as CELLODEXTRIN OXIDASE 2 1 (CELLOX2 CELLOX1), respectively, oxidize mixed-linked β-1→3/β-1→4-glucans (MLGs), recently capable activating plant immunity, reinforcing view BBE-l includes members are devoted control homeostasis potential cell wall-derived damage-associated molecular patterns (DAMPs). putatively paralogous genes display different expression profiles. Unlike CELLOX1, CELLOX2 is not expressed seedlings or adult plants involved immunity against Botrytis cinerea. Both instead a concerted manner seed coat during development. Whereas mainly heart stage, CELLOX1 at immediately later when decreases. Analysis seeds cellox1 cellox2 knockout mutants shows alterations structure: columella area smaller cellox1, radial walls thicker cellox2, mucilage halo reduced cellox2. However, monosaccharide composition significantly altered, suggesting an alteration organization wall, thus notion architecture wall specific organs determined only by dynamics synthesis/degradation main polysaccharides but also its enzymatic oxidation.

Language: Английский

Citations

12
Subcellular localization requirements and specificities for plant immune receptor Toll‐interleukin‐1 receptor signaling DOI Creative Commons
Maud Bernoux, Jian Chen, Xiaoxiao Zhang

et al.

The Plant Journal, Journal Year: 2023, Volume and Issue: 114(6), P. 1319 - 1337

Published: March 18, 2023

SUMMARY Recent work shed light on how plant intracellular immune receptors of the nucleotide‐binding leucine‐rich repeat (NLR) family are activated upon pathogen effector recognition to trigger responses. Activation Toll‐interleukin‐1 receptor (TIR) domain‐containing NLRs (TNLs) induces oligomerization and close proximity TIR domain, which is required for enzymatic activity. TIR‐catalyzed small signaling molecules bind EDS1 heterodimers subsequently activate downstream helper NLRs, function as Ca 2+ permeable channel responses eventually leading cell death. Subcellular localization requirements TNLs partners not well understood, although they understand fully mechanisms underlying NLR early signaling. show diverse subcellular while shows nucleocytosolic localization. Here, we studied impact mislocalization activation different TNLs. In Nicotiana benthamiana , our results suggest that domains isolated from flax L6 Arabidopsis RPS4 SNC1 drives compartments. Nevertheless, both Golgi‐membrane anchored have same in thaliana . By using mislocalized variants EDS1, found autoimmune domain can induce seedling death when present cytosol. However, restricted nucleus, a stunting phenotype but no Our data point out importance thoroughly investigating dynamics TNL fully.

Language: Английский

Citations

11