Transposon-triggered epigenetic chromatin dynamics modulate EFR-related pathogen response DOI Creative Commons
Regina Mencia, A. Arce,

Candela Houriet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 9, 2023

Summary Infectious diseases drive the evolution of wild plants and impact yield in crop plants. Like animals, can sense biotic threats via conserved pathogen-associated patterns (PAMPs). Since an overly robust immune response harm plants, understanding mechanisms for tuning defense responses to appropriate level is vital as we endeavor develop pathogen-resistant crops. In this paper, studied Arabidopsis pattern recognition receptor (PRR) EFR, which senses bacterial EF-Tu. An inverted-repeat transposon ( Ea-IR ) between EFR neighboring XI-k locus controls local chromatin organization, promoting formation a repressive loop. Upon pathogen infection, landscape around Xl-k dynamically changes allow increased transcription. Chromatin opening facilitates passage RNA polymerase II across gene termination site, leading longer transcript that includes sequences. Dicer-like (DCL) enzymes process into small RNAs (sRNAs), reset topology state, attenuating, turn, response, reminiscent attenuation signaling other systems. From evolutionary point view, found natural accessions missing have constitutive " -open" configuration correlates with higher basal levels background resistance pathogens. Collectively, our study offers evidence scenario transposon, expression interact fine-tune responses, both during course infection evolution. Similar gene-associated IRs crops could provide valuable non-coding targets genome editing or assisted plant breeding programs.

Language: Английский

Downstream signaling induced by several plant Toll/interleukin-1 receptor-containing immune proteins is stable at elevated temperature DOI Creative Commons

Héloïse Demont,

Céline Remblière,

Raphaël Culerrier

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(3), P. 115326 - 115326

Published: Feb. 19, 2025

Language: Английский

Citations

0
Shared signals, different fates: Calcium and ROS in plant PRR and NLR immunity DOI Creative Commons

Chanusha Weralupitiya,

Sophie Eccersall, Claudia‐Nicole Meisrimler

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(11), P. 114910 - 114910

Published: Oct. 30, 2024

Language: Английский

Citations

2
Priming of plant’s immune system: the future sustainable approach for tea improvement DOI Creative Commons

Pranita Hazarika,

Harvinder Singh, Debasis Das

et al.

Discover Plants., Journal Year: 2024, Volume and Issue: 1(1)

Published: Nov. 11, 2024

Plant has an inherent or induced immunity to counter any abiotic biotic stress. Such a response is possible due physiological, molecular and cellular adaptations. Combined effect of stress can provoke cross-tolerance in plants, which may be synergistic antagonistic with the involvement protein kinases, transcription factors, phytohormones, reactive oxygen species (ROS). Priming plant's immune system leads accumulation certain biomolecules like phytoalexins PR proteins inside plant system. Exogenous application beta-aminobutyric acid, benzothiadiazole, azelaic acid been reported prime plants. Indian tea industry holds vital position economy. However, there enormous crop loss yearly tea. This happens despite using recommended agrochemicals disease-resistant cultivars. Non judicious chemical practices have created environmental pollution deposition pesticide residues made Therefore, sustainable approach improvement utmost requirement. done by screening followed identifying their pathway genes responsible for enhanced plant. Then candidate gene(s) over-expressed transgenic approach. In present review, we aim address two important issues: (i) system, (ii) encounter stresses priming its

Language: Английский

Citations

2
Diversified host target families mediate convergently evolved effector recognition across plant species DOI
Haseong Kim, Ye Jin Ahn, Hyeonjung Lee

et al.

Current Opinion in Plant Biology, Journal Year: 2023, Volume and Issue: 74, P. 102398 - 102398

Published: June 8, 2023

Language: Английский

Citations

5
Transposon-triggered epigenetic chromatin dynamics modulate EFR-related pathogen response DOI Creative Commons
Regina Mencia, A. Arce,

Candela Houriet

et al.

Nature Structural & Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 27, 2024

Infectious diseases drive wild plant evolution and impact crop yield. Plants, like animals, sense biotic threats through pattern recognition receptors (PRRs). Overly robust immune responses can harm plants; thus, understanding the tuning of defense response mechanisms is crucial for developing pathogen-resistant crops. In this study, we found that an inverted-repeat transposon (EFR-associated IR, Ea-IR) located between loci encoding PRRs ELONGATION FACTOR-TU RECEPTOR (EFR) myosin XI-k (XI-k) in Arabidopsis affects chromatin organization, promoting formation a repressive loop. Upon pathogen infection, changes around EFR correlate with increased transcription. Pathogen-induced opening causes RNA polymerase II readthrough, producing longer, Ea-IR-containing transcript, processed by Dicer-like enzymes into small RNAs, which reset to state attenuating after infection. accessions lacking Ea-IR have higher basal levels resistance pathogens. We show scenario transposon, organization gene expression interact fine-tune responses, during both course infection evolution. Here, authors next receptor (EFR)-encoding controls response.

Language: Английский

Citations

1
MHP1 and MHL generate odd-chain fatty acids from 2-hydroxy fatty acids in sphingolipids and are related to immunity in Arabidopsis thaliana DOI

Marina Ushio,

Toshiki Ishikawa,

Takakazu Matsuura

et al.

Plant Science, Journal Year: 2023, Volume and Issue: 336, P. 111840 - 111840

Published: Aug. 22, 2023

Language: Английский

Citations

2
Revealing the Complete Bispecific Phosphatase Genes (DUSPs) across the Genome and Investigating the Expression Patterns of GH_A11G3500 Resistance against Verticillium wilt DOI Open Access

Yahui Deng,

Xiaojuan Deng,

Jieyin Zhao

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4500 - 4500

Published: April 19, 2024

DUSPs, a diverse group of protein phosphatases, play pivotal role in orchestrating cellular growth and development through intricate signaling pathways. Notably, they actively participate the MAPK pathway, which governs crucial aspects plant physiology, including regulation, disease resistance, pest stress response. DUSP is key enzyme, it enzyme that limits rate cell metabolism. At present, complete understanding gene family cotton its specific roles resistance to Verticillium wilt (VW) remains elusive. To address this knowledge gap, we conducted comprehensive identification analysis four species: Gossypium arboreum, barbadense, hirsutum, raimondii. The results revealed total 120 genes varieties, were categorized into six subgroups randomly distributed at both ends 26 chromosomes, predominantly localized within nucleus. Our demonstrated closely related exhibited similarities terms conserved motif composition structure. A promoter performed on GhDUSP presence several cis-acting elements, are associated with abiotic biotic responses, as well hormone signaling. tissue expression pattern significant variations under different conditions, roots exhibiting highest levels, followed by stems leaves. In tissue-specific detection, petals, leaves, stems, stamens, receptacles higher levels gene. for GhDUSPs suggest may have response cotton. Through Virus-Induced Gene Silencing (VIGS) experiments, silencing target significantly reduced efficiency disease-resistant varieties against (VW). Consequently, conclude GH_A11G3500-mediated bispecific phosphorylated serve regulators G. hirsutum This study presents structure designed provide an in-depth potential biological functions cotton, providing strong foundation further research molecular breeding pathogens.

Language: Английский

Citations

0
Immune signaling induced by plant Toll/interleukin-1 receptor (TIR) domains is thermostable DOI Creative Commons

Héloïse Demont,

Céline Remblière,

Laurent Deslandes

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 13, 2024

Summary Plant disease is a major threat in agriculture and climate change predicted to intensify it. Above the optimal plant’s growth range, plant immunity particular immune responses induced by nucleotide-binding leucine rich repeat receptors (NLRs) are dampened, but underlying molecular mechanisms remains elusive. NLRs usually contain an N-terminal signaling domain, such as Toll/interleukin-1 receptor (TIR) which self-sufficient trigger signaling. By using inducible Arabidopsis transgenic lines expressing TIR-containing (TNLs) or corresponding isolated TIR domains from RPS4 flax L6 NLRs, we showed that downstream of TNL activation not affected elevation temperature. Conditional TNL- TIR-mediated follow same route at permissive temperature (EDS1/RNLs requirement salicylic acid sector). Yet, this pathway maintained under elevated (30°C) when TIRs, full-length TNLs. This work underlines need further study how impacted increase temperature, particularly important improve resilience resistance warming climate.

Language: Английский

Citations

0
Genetic Mapping and Characterization of the Clubroot Resistance Gene BraPb8.3 in Brassica rapa DOI Open Access
Liyan Kong, Yi Yang,

Yufei Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10462 - 10462

Published: Sept. 28, 2024

Clubroot, a significant soil-borne disease, severely impacts the productivity of cruciferous crops. The identification and development clubroot resistance (CR) genes are crucial for mitigating this disease. This study investigated genetic inheritance within an F2 progeny derived from cross resistant parent, designated “377”, susceptible “12A”. Notably, “377” exhibited robust to “KEL-23” strain Plasmodiophora brassicae, causative agent clubroot. Genetic analyses suggested that observed is controlled by single dominant gene. Through Bulked Segregant Analysis sequencing (BSA-seq) preliminary gene mapping, we localized CR locus, as BraPb8.3, 1.30 Mb genomic segment on chromosome A08, flanked markers “333” “sau332-1”. Further fine mapping precisely narrowed down position BraPb8.3 173.8 kb region between “srt8-65” “srt8-25”, where identified 22 genes, including Bra020861 with TIR-NBS-LRR domain Bra020876 LRR domain. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) confirmed both exhibit increased expression levels in parent following inoculation P. thereby underscoring their potential key implicated BraPb8.3-mediated resistance. not only identifies molecular associated but also enriches resources available breeding programs aimed at enhancing

Language: Английский

Citations

0
Plant-Microbe Interactions, Signalling Events, and Cellular Networks Against Harmful Pathogens DOI

Tamanna Bhardwaj,

Deepak Kumar,

Parkirti Parkirti

et al.

Sustainability in plant and crop protection, Journal Year: 2024, Volume and Issue: unknown, P. 37 - 57

Published: Jan. 1, 2024

Language: Английский

Citations

0