Magainin-AM2 inhibits sucrose-induced hyperglycaemia, oxidative stress, and cognitive dysfunction in Drosophila melanogaster DOI Creative Commons

Adeola Adebowale,

Zeniat Oyaluna,

Ayodele A. Falobi

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 222, P. 414 - 423

Published: July 2, 2024

Hyperglycaemia-induced oxidative stress plays significant roles in the development of type 2 diabetes and its complications. This study investigates effects magainin-AM2 on high-sucrose diet induced redox imbalance cognitive impairment Drosophila melanogaster. Effects various concentrations sucrose, or a combination both agents mortality, eclosion rate, generation reactive oxygen nitrogen species, activities antioxidant enzymes, thiol system, markers functions control treated flies were examined. Results showed that exposure to high sucrose (30 %–60 % w/w) increased mortality rate (38–67 %, P < 0.001) levels glucose (1.8–1.9-fold, 0.001), hydrogen peroxide (1.4–1.5-fold, 0.01) nitrite/nitrate (1.2-fold, 0.01). Decreased total (53–59 0.01), non-protein thiols (59–63 catalase (39–47 0.01–0.05) glutathione-s-transferase (31–43 also observed. Magainin-AM2 (0–10 μM/kg diet) did not affect fly nitrite/nitrate, glutathione-s-transferase. However, peptide produced dose-dependent increase 1.2–1.6-fold, 0.001–0.01)and increases at 10 (2.0-fold, inhibited sucrose-induced elevation (55–70 (11–12 (20–34 0.01–0.05). The prevented reduction (1.9–2.0-fold, 0.05) (2.3–3.1-fold, (1.8–2.8-fold, 0.001–0.05). acetylcholinesterase (3.6–4.0-fold, (18 negative geotaxis (1.3–14-fold, 0.001). These results indicate beneficial actions may involve prevention hyperglycaemia-induced damage encourage further as an anti-diabetic agent.

Language: Английский

Novel Tryptophyllin Peptides from Physalaemus centralis Inhibit Oxidative Stress-Induced Endothelial Dysfunction in Rat Aorta Preparation DOI

Ariane Nogueira,

José Brango‐Vanegas,

Andreanne G. Vasconcelos

et al.

Toxicon, Journal Year: 2025, Volume and Issue: unknown, P. 108234 - 108234

Published: Jan. 1, 2025

Language: Английский

Citations

0
Exploring alternative sources of protein in food: Trends in nutrient and functional features DOI

A I Lara-Parra,

Aldahir Alberto Hernández Hernández, Yari Jaguey-Hernández

et al.

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116224 - 116224

Published: March 1, 2025

Language: Английский

Citations

0
Natural Antidiabetic Agents: Current Evidence and Development Pathways from Medicinal Plants to Clinical use DOI Creative Commons
Daniel Ejim Uti, Item Justin Atangwho, Esther Ugo Alum

et al.

Natural Product Communications, Journal Year: 2025, Volume and Issue: 20(3)

Published: March 1, 2025

Diabetes mellitus is a long-term metabolic condition marked by chronically high levels of glucose in the blood due to insufficient synthesis insulin or impaired function. Throughout history, several civilizations have used traditional medicinal plants treat and control diabetes. This paper offers thorough review, date, antidiabetic natural compounds origin, specifically highlighting their reported modes mechanisms action therapeutic possibilities, with view fast-tracking possibility transition pharmaceutical products for human use. Phytochemicals such as flavonoids, alkaloids, terpenoids, glycosides notable effects. They been shown via many processes, including boosting secretion, improving sensitivity, blocking absorption \gastrointestinal-tract, regulating carbohydrate metabolism. Unlike earlier reviews, this current one, addition chemistry proposed action, also x-rays effectiveness safety these chemicals combining evidences from laboratory research, animal experiments, clinical trials. Several substances, Demethoxycurcumin, Trigonelline, Ginsenosides, progressed trials, while others like Allicin Polypeptide-p at bedside. Bisdemethoxycurcumin, Vicine, Gymnemosides, are now preclinical trial phase, suggesting that research still ongoing there promise future application. The review highlights evolution diabetes management, potential improve treatment. It suggests incorporating plant-derived into treatments, reducing dependence on drugs adverse

Language: Английский

Citations

0
Multifunctional host-defense peptides isolated from skin secretions of the banana tree dwelling frog Boana platanera (Hylidae; Hylinae) DOI Creative Commons
J. Michael Conlon, Ananyaa Sridhar, Dawood Khan

et al.

Biochimie, Journal Year: 2024, Volume and Issue: 223, P. 23 - 30

Published: March 30, 2024

Five host-defense peptides (figainin 2PL, hylin PL, raniseptin plasticin and peptide YL) were isolated from norepinephrine-stimulated skin secretions of the banana tree dwelling frog Boana platanera (Hylidae; Hylinae) collected in Trinidad. Raniseptin PL (GVFDTVKKIGKAVGKFALGVAKNYLNS.NH2) figainin 2PL (FLGTVLKLGKAIAKTVVPMLTNAMQP KQ.NH2) showed potent rapid bactericidal activity against a range clinically relevant Gram-positive Gram-negative ESKAPE + pathogens Clostridioides difficile. The also cytotoxic (LC50 values < 30 μM) A549, MDA-MB-231 HT29 human tumor-derived cell lines but appreciably lower hemolytic mouse erythrocytes = 262 ± 14 μM for 157 16 2PL). Hylin (FLGLIPALAGAIGNLIK.NH2) relatively weak microorganisms was more hemolytic. glycine-leucine-rich with structural similarity to plasticins (GLLSTVGGLVGGLL NNLGL.NH2) non-cytotoxic YL (YVPGVIESLL.NH2) lacked antimicrobial activities. raniseptin-PL stimulated rate release insulin BRIN-BD11 clonal β-cells at concentrations ≥100 nM. Peptide most effective (2.3-fold increase compared basal 1 concentration) may represent template design new class incretin-based anti-diabetic drugs.

Language: Английский

Citations

1
Magainin-AM2 inhibits sucrose-induced hyperglycaemia, oxidative stress, and cognitive dysfunction in Drosophila melanogaster DOI Creative Commons

Adeola Adebowale,

Zeniat Oyaluna,

Ayodele A. Falobi

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 222, P. 414 - 423

Published: July 2, 2024

Hyperglycaemia-induced oxidative stress plays significant roles in the development of type 2 diabetes and its complications. This study investigates effects magainin-AM2 on high-sucrose diet induced redox imbalance cognitive impairment Drosophila melanogaster. Effects various concentrations sucrose, or a combination both agents mortality, eclosion rate, generation reactive oxygen nitrogen species, activities antioxidant enzymes, thiol system, markers functions control treated flies were examined. Results showed that exposure to high sucrose (30 %–60 % w/w) increased mortality rate (38–67 %, P < 0.001) levels glucose (1.8–1.9-fold, 0.001), hydrogen peroxide (1.4–1.5-fold, 0.01) nitrite/nitrate (1.2-fold, 0.01). Decreased total (53–59 0.01), non-protein thiols (59–63 catalase (39–47 0.01–0.05) glutathione-s-transferase (31–43 also observed. Magainin-AM2 (0–10 μM/kg diet) did not affect fly nitrite/nitrate, glutathione-s-transferase. However, peptide produced dose-dependent increase 1.2–1.6-fold, 0.001–0.01)and increases at 10 (2.0-fold, inhibited sucrose-induced elevation (55–70 (11–12 (20–34 0.01–0.05). The prevented reduction (1.9–2.0-fold, 0.05) (2.3–3.1-fold, (1.8–2.8-fold, 0.001–0.05). acetylcholinesterase (3.6–4.0-fold, (18 negative geotaxis (1.3–14-fold, 0.001). These results indicate beneficial actions may involve prevention hyperglycaemia-induced damage encourage further as an anti-diabetic agent.

Language: Английский

Citations

0