Blue
mold
induced
by
Penicillium
choerospondiatis
is
a
primary
cause
of
growth
and
postharvest
losses
in
the
fruit
Phyllanthus
emblica.
There
an
urgent
need
to
explore
novel
safe
fungicides
control
this
disease.
Here,
we
demonstrated
osthole,
natural
coumarin
compound
isolated
from
Cnidium
monnieri,
exhibited
strong
inhibitory
effect
on
mycelia
growth,
conidial
germination
rate
germ
tube
length
P.
choerospondiatis,
effectively
suppressed
blue
development
fruit.
The
median
effective
concentration
osthole
was
9.86mg/L.
Osthole
treatment
resulted
structural
disruption
cell
wall
autophagic
vacuoles
containing
cytoplasmic
components
fungal
cells.
Transcriptome
analysis
revealed
that
led
differentially
expressed
genes
mainly
enriched
synthesis,
TCA
cycle,
glycolysis/
gluconeogenesis,
oxidative
phosphorylation.
Moreover,
increase
expression
involved
peroxisome,
autophagy
endocytosis.
Particularly,
pathway
related
(PcATG1,
PcATG3,
PcATG15,
PcATG27,
PcYPT7and
PcSEC18)
were
prominently
up-regulated
osthole.
Summarily,
these
results
indicated
inducing
activity.
has
potentials
develop
as
antifungal
agent
for
controlling
disease
fruits.
Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(39), P. 14151 - 14163
Published: Sept. 25, 2023
To
discover
novel
natural
product-based
fungicidal
agrochemicals,
41
camphanic
acid
hydrazide
and
camphor
sulfonamide
derivatives
were
designed,
synthesized,
tested
for
their
antifungal
profile
against
four
plant
pathogenic
fungi
three
oomycetes.
As
a
result,
some
presented
pronounced
inhibitory
activities
toward
Botryosphaeria
dothidea,
Fusarium
graminearum,
Phytophthora
capsici,
nicotianae.
Especially,
compound
4b
demonstrated
the
most
potent
anti-B.
dothidea
activity
(EC50
=
1.28
mg/L),
much
stronger
than
positive
control
chlorthalonil.
The
in
vivo
assay
showed
that
displayed
significant
protective
curative
effects
on
apple
fruits
infected
by
B.
dothidea.
primary
mechanism
study
revealed
could
obviously
enhance
cell
membrane
permeability,
destroy
mycelial
surface
morphology
ultrastructure,
reduce
ergosterol
exopolysaccharide
contents
of
Further,
laccase
vitro
with
an
IC50
value
11.3
μM,
superior
to
cysteine.
molecular
docking
dock
well
into
active
site
forming
multiple
interactions
key
residues
pocket.
acute
oral
toxicity
test
rats
had
slight
LD50
849.1
mg/kg
bw
(95%
confidence
limit:
403.9-1785.3
bw).
This
research
identified
be
promising
leads
development
laccase-targeting
fungicides.
Advanced Functional Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 28, 2025
Abstract
The
development
of
multi‐action
pesticides
presents
significant
advantages,
including
cost‐effectiveness,
reduced
application
frequency,
enhanced
resistance
management,
and
minimized
environmental
impact.
Despite
its
efficacy
in
targeting
chitin
synthase
both
fungi
insects,
the
insecticidal
performance
polyoxin
B
remains
limited.
To
overcome
this
limitation,
a
novel
nano‐formulation,
B@ZTS
is
developed,
utilizing
agricultural
byproducts,
tea
saponin,
zein.
This
formulation
features
small
particle
size,
high
leaf
deposition
efficiency,
excellent
dispersion
properties.
Leveraging
disulfide
bond
system,
designed
to
respond
intracellular
reducing
environment,
enabling
controlled
release
B.
Remarkably,
nano‐formulation
facilitates
penetration
physiological
barriers,
achieving
equivalent
fungicidal
at
only
one‐fifth
dosage.
work
highlights
potential
eco‐friendly,
cost‐effective,
multifunctional
nano‐pesticides,
providing
compelling
solution
for
sustainable
practices
demonstrating
broad
applicability
integrated
pest
disease
management.
Journal of Agricultural and Food Chemistry,
Journal Year:
2023,
Volume and Issue:
71(46), P. 17723 - 17732
Published: Nov. 8, 2023
Ustilaginoidea
virens
is
a
destructive
phytopathogenic
fungus
that
causes
false
smut
disease
in
rice.
In
this
study,
the
natural
product
2,4-di-tert-butylphenol
(2,4-DTBP)
was
found
to
be
an
environmentally
friendly
and
effective
agent
for
first
time,
which
exhibited
strong
antifungal
activity
against
U.
virens,
with
EC50
value
of
0.087
mmol/L.
The
scanning
electron
microscopy,
fluorescence
staining,
biochemical
assays
indicated
2,4-DTBP
could
destroy
cell
wall,
membrane,
cellular
redox
homeostasis
ultimately
resulting
fungal
death.
Through
transcriptomic
analysis,
total
353
genes
were
significantly
upregulated
367
downregulated,
focusing
on
spindle
microtubule
assembly,
wall
homeostasis,
mycotoxin
biosynthesis,
intracellular
metabolism.
These
results
enhanced
understanding
action
mechanisms
supporting
it
potential
control
disease.
Food and Energy Security,
Journal Year:
2024,
Volume and Issue:
13(4)
Published: July 1, 2024
Abstract
Half
of
the
world's
population
depends
on
rice
for
their
calories.
Protecting
in
growth
period
from
damage
caused
by
phytopathogens
is
faced
with
a
great
challenge
under
frequent
extreme
climate.
To
find
novel
fungicides
to
control
diseases,
35
phenylthiazole‐1,3,4‐oxadiazole‐thioether
(sulfone)
derivatives
were
synthesized
and
evaluated
efficacy
against
destructive
fungal
bacterial
diseases
rice.
Bioassay
results
demonstrated
that
most
G‐series
compounds
possessed
excellent
antifungal
antibacterial
activities.
In
particular,
G1
(EC
50
=
2.22
μg/mL,
R.s
)
G7
2.76
showed
promising
activities
vitro
exhibited
superior
protective
curative
sheath
blight
vivo
compared
commercial
carbendazim.
Surprisingly,
compound
G2
remarkable
activity
Xanthomonas
oryzae
pv.
(
Xoo
an
EC
value
1.98
(88.08%)
than
thiodiazole
copper
(79.39%)
leaf
at
200
μg/mL.
The
abovementioned
fully
manifested
phenylthiazole‐1,3,4‐oxadiazole‐sulfone
structure,
especially
,
had
potential
develop
as
agents
controlling
diseases.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(32), P. 18027 - 18044
Published: July 30, 2024
Botrytis
cinerea
(B.
cinerea)
and
Colletotrichum
gloeosporioides
(C.
gloeosporioides)
were
isolated
from
the
decaying
strawberry
tissue.
The
antifungal
properties
of
Monarda
didyma
essential
oil
(MEO)
its
nanoemulsion
confirmed,
demonstrating
complete
inhibition
pathogens
at
concentrations
0.45
μL/mL
(0.37
mg/mL)
10
μL/mL,
respectively.
Thymol,
a
primary
component
MEO,
was
determined
as
an
antimicrobial
agent
with
IC50
values
34.51
53.40
μg/mL.
Hippophae
rhamnoides
(HEO)
confirmed
potent
antioxidant,
leading
to
development
thymol-HEO-chitosan
film
designed
act
antistaling
agent.
disease
index
weight
loss
rate
can
be
reduced
by
90
60%,
respectively,
nutrients
also
being
well-preserved,
offering
innovative
approach
preservative
development.
Studies
on
mechanism
revealed
that
thymol
could
bind
FKS1
disrupt
cell
wall,
causing
collapse
mitochondrial
membrane
potential
burst
reactive
oxygen
species.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 25, 2024
To
develop
further
novel
environmentally
friendly
antifungal
agents
with
high
efficacy,
a
series
of
pyrazole-5-sulfonamide
derivatives
were
designed
and
synthesized
by
using
the
active
molecules
in
previous
works
as
lead
compounds.
Their
activities
evaluated
vitro
against
ten
highly
destructive
plant
pathogenic
fungi.
The
bioassay
results
indicated
that
more
than
half
target
compounds
displayed
potent
(inhibition
rate
≥85%)
