Antifungal Activity and Mechanism of Camphor Derivatives against Rhizoctonia solani: A Promising Alternative Antifungal Agent for Rice Sheath Blight

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(20), P. 11415 - 11428

Published: May 10, 2024

Rice sheath blight, caused by the fungus Rhizoctonia solani, poses a significant threat to rice cultivation globally. This study aimed investigate potential mechanisms of action camphor derivatives against R. solani. Compound 4o exhibited superior fungicidal activities in vitro (EC50 = 6.16 mg/L), and vivo curative effects (77.5%) at 500 mg/L were significantly (P < 0.01) higher than positive control validamycin·bacillus (66.1%). Additionally, compound low cytotoxicity acute oral toxicity for adult worker honeybees Apis mellifera L. Mechanistically, disrupted mycelial morphology microstructure, increased cell membrane permeability, inhibited both PDH SDH enzyme activities. Molecular docking molecular dynamics analyses indicated tight interaction with active sites. In summary, substantial antifungal efficacy serving as promising lead further optimization agents.

Language: Английский

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Structural Simplification of Luotonin F: Discovery of Quinoline Derivatives as Novel Antifungal Agents for Plant Protection

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Inspired by the natural quinoline alkaloid Luotonin F, a series of quinoline-3-hydrazide derivatives were designed and synthesized structure simplification evaluated for their fungicidal activities against plant phytopathogenic fungi. The bioassay results showed that these compounds exhibited broad-spectrum highly efficient antifungal in vitro. Among them, compound W9 inhibitory R. solani, S. sclerotiorum, B. cinerea, F. graminearum, oxysporum, P. capsici with EC50 values 0.471, 0.752, 0.570, 0.329, 0.960, 0.504 μg/mL, respectively. In particular, was more effective cinerea than reference drugs Pyrimethanil (EC50 = 3.54 μg/mL) Boscalid 1.37 μg/mL). Therefore, preliminary mechanism action on explored. it could affect mycelial morphology, disrupt integrity cell membrane, increase reactive oxygen species content. Furthermore, excellent vivo protection efficacy compared to drug Pyrimethanil. Thus, is expected be novel candidate, exhibiting activities, which worthy further investigation.

Language: Английский

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Design, synthesis, antifungal evaluation and mechanism study of novel norbornene derivatives as potential laccase inhibitors

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(9), P. 4273 - 4285

Published: April 16, 2024

Abstract BACKGROUND To discover novel fungicide candidates, five series of norbornene hydrazide, bishydrazide, oxadiazole, carboxamide and acylthiourea derivatives ( 2a‐2t , 3a‐3f, 4a‐4f, 5a‐5f 7a‐7f ) were designed, synthesized assayed for their antifungal activity toward seven representative plant fungal pathogens. RESULTS In the in vitro assay, some title presented good against Botryosphaeria dothidea Sclerotinia sclerotiorum Fusarium graminearum . Especially, compound 2b exhibited best inhibitory B. with median effective concentration (EC 50 0.17 mg L −1 substantially stronger than those reference fungicides boscalid carbendazim. The vivo assay on apples revealed that had significant curative protective effects, both which superior to boscalid. preliminary mechanism study, was able injure surface morphology hyphae, destroy cell membrane integrity increase intracellular reactive oxygen species (ROS) level addition, could considerably inhibit laccase (IC 1.02 μM, much positive control cysteine = 35.50 μM). binding affinity interaction mode also confirmed by molecular docking. CONCLUSION This study a promising lead inhibitors as fungicidal agrochemicals, demonstrate anti‐ activity. © 2024 Society Chemical Industry.

Language: Английский

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Thin layer chromatography assay to detect laccase inhibitors

Food Chemistry, Journal Year: 2024, Volume and Issue: 460, P. 140583 - 140583

Published: July 24, 2024

Language: Английский

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Isolating Antipathogenic Fungal Coumarins from Coriaria nepalensis and Determining Their Primary Mechanism In Vitro

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(12), P. 6711 - 6722

Published: March 16, 2024

Through bioassay-guided isolation, eight undescribed coumarins (1–8), along with six reported (9–14), were obtained from Coriaria nepalensis. The new structures determined by using IR, UV, NMR, HRESIMS, and ECD calculations. results of the biological activity assays showed that compound 9 exhibited broad spectrum antifungal activities against all tested fungi in vitro a significant inhibitory effect on Phytophthora nicotianae an EC50 value 3.00 μg/mL. Notably, demonstrated greater curative protective effects tobacco balack shank than those osthol vivo. Thus, was structurally modified to obtain promising agents, novel derivatives (17b, 17j, 17k) better Sclerotinia sclerotiorum did lead 9. Preliminary mechanistic exploration illustrated could enhance cell membrane permeability, destroy morphology ultrastructure cells, reduce exopolysaccharide content P. mycelia. Furthermore, cytotoxicity revealed relatively low HEK293 lines inhibition rate 33.54% at 30 This research is for discovery fungicides natural satisfactory ecological compatibility.

Language: Английский

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Synthesis and antifungal activity of arecoline derivatives containing amino acid fragments

Molecular Diversity, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Language: Английский

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A Highly Sensitive Caffeic Acid Fluorescent Probe for Detecting Laccase in Grape Juice and Mushrooms

The Analyst, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

trans -Caffeic acid was used as a fluorescent probe for highly sensitive detection of laccase. Laccase contents in three grape juice samples with different fermentation levels and kinds mushroom extracts were determined.

Language: Английский

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Design and Synthesis of 3-Carene-Derived Amide-Thiourea/Nanochitosan Complexes with Excellent Laccase Inhibitory Activity and Sustained Releasing Performance for Crop Protection

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 19, 2024

The discovery of natural product-derived novel nanopesticide systems can effectively address the adverse effects caused by improper use traditional fungicides. In this research, 33 3-carene-derived amide-thiourea derivatives

Language: Английский

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Design and synthesis of bis(indolyl)-hydrazide-hydrazone derivatives and their antifungal activities against plant pathogen fungi

Natural Product Research, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 6

Published: June 28, 2024

A series of bis(indolyl)-hydrazide-hydrazone derivatives were synthesised, and their structures characterised using

Language: Английский

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Pinonic Acid Derivatives Containing Thiourea Motif: Promising Antifungal Lead Compound Targeting Cellular Barrier of Colletotrichum fructicola

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

In order to explore novel antifungal lead compounds from plant essential oil, thirty-two pinonic acid derivatives containing thiourea groups were designed and synthesized using α-pinene as a raw material. One of these compound

Language: Английский

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