Revealing the mechanism: the influence of Baicalin on M1/M2 and Th1/Th2 imbalances in mycoplasma gallisepticum infection DOI Creative Commons

Yuquan Guo,

Yusong Miao,

Hao Chen

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(10), P. 104145 - 104145

Published: July 30, 2024

Mycoplasma gallisepticum (MG) is a pathogen that induces chronic respiratory illnesses in chickens, leading to tracheal and lung injury, eliciting immune reactions support sustained colonization. Baicalin, compound found scutellaria baicalensis, exhibits anti-inflammatory, antioxidant, antibacterial properties. This study aimed investigate the potential of baicalin alleviating cell damage caused by MG restoring imbalances M1/M2 Th1/Th2 differentiation explore its underlying mechanism. In this research, model for polarization induced was initially developed. Specifically, infection with at multiplicity (MOI) 400 6 h represented M1 model, while 10 M2 model. The markers were subsequently validated using qRT-PCR, ELISA, Western blot analysis. Baicalin disrupts activation cells has restore balance between cells, thereby mitigating inflammatory resulting from MG. Subsequent studies on MG-infected chickens detected alveolar lavage fluid, as well macrophages Th lung. M1/Th1 exposed 5 d, M2/Th2 infected 7 d. utilization both light electron transmission microscopes revealed administration resulted reduction number decrease cytoplasmic vacuoles, restoration mitochondrial swelling chromatin agglutination, alleviation rupture infiltration. Furthermore, restored MG-induced inhibited phosphorylation p38 p65 proteins, hindering TLR4-p38 MAPK/NF-κB pathway. provides insights into long-term effects offers theoretical basis practical applications.

Language: Английский

Current Advances in the Therapeutic Potential of Scutellarin: Novel Applications, Mechanisms, and Future Challenges. DOI Creative Commons
Great Iruoghene Edo, Alice Njolke Mafe, Patrick Othuke Akpoghelie

et al.

Phytomedicine Plus, Journal Year: 2025, Volume and Issue: unknown, P. 100754 - 100754

Published: Jan. 1, 2025

Language: Английский

Citations

2
The protective role of baicalin regulation of autophagy in cancers DOI Creative Commons
Qí Zhāng,

Shiyun Guo,

Han Ge

et al.

Cytotechnology, Journal Year: 2025, Volume and Issue: 77(1)

Published: Jan. 3, 2025

Language: Английский

Citations

1
Baicalin attenuates the immune escape of oral squamous cell carcinoma by reducing lactate accumulation in tumor microenvironment DOI Creative Commons

Yameng Cui,

Jingwen Liu, Xi Wang

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Antitumor Research Based on Drug Delivery Carriers: Reversing the Polarization of Tumor-Associated Macrophages DOI
Xinyu Cao,

Shen Wan,

Bingyu Wu

et al.

Molecular Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

The development of malignant tumors is a complex process that involves the tumor microenvironment (TME). An immunosuppressive TME presents significant challenges to current cancer therapies, serving as key mechanism through which cells evade immune detection and play crucial role in progression metastasis. This impedes optimal effectiveness immunotherapeutic approaches, including cytokines, checkpoint inhibitors, vaccines. Tumor-associated macrophages (TAMs), major component tumor-infiltrating cells, exhibit dual functionalities: M1-like TAMs suppress tumorigenesis, while M2-like promote growth Consequently, various nanocarriers aimed at polarizing phenotypes distinct mechanisms has emerged promising therapeutic strategy inhibit escape enhance antitumor responses. Review covers origin types TAMs, common pathways regulating macrophage polarization, progression, strategies targeting aiming provide comprehensive understanding guidance for future research clinical applications.

Language: Английский

Citations

1
Baicalin inhibits LPS-induced apoptosis and inflammation in WI- 38 cells by promoting FOXA2/TRIM27 Interaction: Implications for pediatric pneumonia mechanisms DOI
Lihua Gao, Xianyi Zeng, Yubo Huang

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

Citations

1
Traditional Chinese medicine for the treatment of cancers of hepatobiliary system: from clinical evidence to drug discovery DOI Creative Commons
Junyu Wu, Guping Tang, Chien‐shan Cheng

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Oct. 1, 2024

Language: Английский

Citations

7
Restoring bone-fat equilibrium: Baicalin's impact on P38 MAPK pathway for treating diabetic osteoporosis DOI Open Access

Weicheng Qin,

Qi Shang,

Gengyang Shen

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 175, P. 116571 - 116571

Published: April 26, 2024

Diabetes can lead to a disorder of bone-fat balance, significant cause osteoporosis due changes in environmental factors. Baicalin (Bai), an active ingredient Scutellaria baicalensis, has been confirmed possess antioxidant, hypoglycemic, and anti-osteoporotic effects. However, comprehensive understanding Bai's influence on diabetic (DOP), including its effects underlying mechanisms, remains elusive. This study investigated impact the equilibrium rats with DOP. The results indicated that Bai alleviated bone damage DOP by promoting osteogenesis inhibiting adipogenesis. Concurrently, through bioinformatics analysis, it was suggested mechanism action might involve P38-MAPK pathway. In vitro, found enhance development marrow mesenchymal stem cells (BMSCs) towards osteogenic lineages while suppressing their differentiation adipogenic lineages. It discovered promotion BMSC depends Additionally, synergistic effect mediated inhibitor suppressed differentiation. Our research indicates pathway play role osteogenic-adipogenic BMSCs, showcasing potential for treatment. highlights ability regulate between fat, presenting novel approach adressing

Language: Английский

Citations

5
Natural Products with Anti-tumorigenesis Potential Targeting Macrophage DOI
Hao Liu,

Manru Huang,

Dandan Xin

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 131, P. 155794 - 155794

Published: June 1, 2024

Language: Английский

Citations

5
Baicalein suppresses Coxsackievirus B3 replication by inhibiting caspase-1 and viral protease 2A DOI Creative Commons

Yanyan Dong,

Enze Shao, Siwei Li

et al.

Virologica Sinica, Journal Year: 2024, Volume and Issue: 39(4), P. 685 - 693

Published: July 16, 2024

Myocarditis is an inflammatory disease of the cardiac muscle and one primary causes dilated cardiomyopathy. Group B coxsackievirus (CVB) leading causative pathogens viral myocarditis, which primarily affects children young adults. Due to lack vaccines, development antiviral medicines crucial controlling CVB infection progression myocarditis. In this study, we investigated effect baicalein, a flavonoid extracted from Scutellaria baicaleinsis. Our results demonstrated that baicalein treatment significantly reduced cytopathic increased cell viability in CVB3-infected cells. addition, significant reductions protein 3D, RNA, particles were observed cells treated with baicalein. We found exerted its inhibitory early stages CVB3 infection. Baicalein also suppressed replication myocardium effectively alleviated myocarditis induced by study revealed exerts inhibiting activity caspase-1 protease 2A. Taken together, our findings demonstrate has against may serve as potential therapeutic option for caused enterovirus

Language: Английский

Citations

4
Preparation of Biomimetic Selenium–Baicalein Nanoparticles and Their Targeted Therapeutic Application in Nonsmall Cell Lung Cancer DOI
Huan Shi,

Biaobiao Wang,

Huilin Ma

et al.

Molecular Pharmaceutics, Journal Year: 2024, Volume and Issue: 21(9), P. 4476 - 4489

Published: Aug. 6, 2024

In this study, we prepared bionic selenium-baicalein nanoparticles (ACM-SSe-BE) for the targeted treatment of nonsmall cell lung cancer. Due to coating A549 membrane, system has homologous targeting capabilities, allowing preparation target tumor cells. The borate ester bond between selenium (SSe) and baicalein (BE) is pH-sensitive can break under acidic conditions in microenvironment achieve release BE at site. Moreover, SSe further enhances antitumor effect by increasing production ROS Transmission electron microscopy (TEM) images dynamic light scattering (DLS) showed that ACM-SSe-BE had a particle size approximately 155 ± 2 nm. FTIR verified successful coupling BE. vitro experiments indicated cumulative pH 5.5 after 24 h was 69.39 1.07%, which less than 20% 7.4, confirming ACM-SSe-BE. Cell uptake vivo imaging good ability. results MTT, flow cytometry, Western blot, immunofluorescence staining demonstrated promoted apoptosis inhibited proliferation. were consistent with those experiments. These clearly suggested will be promising nanosystem

Language: Английский

Citations

4