Single-Cell Sequencing Combined with Transcriptome Sequencing to Explore the Molecular Mechanisms Related to Skin Photoaging DOI Creative Commons
Xinru Hu, Shuang Du, Meng Chen

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 11137 - 11160

Published: Dec. 1, 2024

Background:The aging of skin is a diversified biological phenomenon, influenced by combination genetic and environmental factors.However, the specific mechanism photoaging not yet completely elucidated.Methods: Gene expression profiles for patients were obtained from Expression Omnibus (GEO) collection.We conducted single-cell intercellular communication investigations to identify potential gene sets.Predictive models created using LASSO regression.The relationships between genes immune cells investigated single sample set enrichment analysis (ssGSEA) variance (GSVA).The molecular processes important studied analysis.A miRNA network was look target miRNAs connected with genes, transcriptional regulation used related transcription factors.Finally, merging co-expression networks drug prediction shows pathways treatment targets.Furthermore, we validated role key cell infiltration, Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) pathway in photoaging, which identified through bioinformatics analysis, vivo reverse quantitative PCR (RT-qPCR), immunofluorescence labeling, Western blotting.Results: This study discovered three including Atp2b1, Plekho2, Tspan13, perform crucial functions process.Immune infiltration showed increased M1 macrophages CD4 memory T group.Further signaling indicated that these are enriched multiple metabolic pathways.The significant roles AMPK vivo. Conclusion:This research revealed underlying mechanisms indicating such as Atp2b1 Tspan13 play pathways.These findings provide new theory prospective targets advancement relevant drugs.

Language: Английский

Qishen granule alleviates doxorubicin-induced cardiotoxicity by suppressing ferroptosis via nuclear erythroid factor 2-related factor 2 (Nrf2) pathway DOI
Siming Xue, Huan Chen, Jingmei Zhang

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 335, P. 118604 - 118604

Published: July 22, 2024

Language: Английский

Citations

7
A look at MERCs as UPRmt regulatory hubs in age-associated heart diseases DOI Open Access

Gabriela Navarrete-Anastasio,

Zeltzin Alejandra Ceja-Galicia, Cecilia Zazueta

et al.

The Journal of Cardiovascular Aging, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 22, 2025

With the increase in life expectancy globally, challenge of dealing with aging becomes more prominent. Aging is a risk factor for several diseases, including cardiovascular disease. Mitochondria, which have long been studied relation to aging, play crucial role maintaining cellular homeostasis. However, there limitation interorganellar communication as organisms age. The unfolded protein response mitochondria (UPRmt) activated during stress maintain mitochondrial homeostasis and prevent accumulation damaged mitochondria. This involves signaling from nucleus, leading transcriptional changes. In context heart, this review explores terms function morphology. It also discusses impact UPRmt on cardiac diseases such heart failure, acute myocardial infarction, dilated cardiomyopathy. highlights potential mitochondria-endoplasmic reticulum contact sites (MERCs) modulating aging. Finally, it provides an update molecules that induce activity, potentially benefiting

Language: Английский

Citations

0
Application of multi-needle injector for delivering human adipose stem cells to combat photoaging in nude mice DOI
Li Tian,

Shimeng Wang,

Duo Zhang

et al.

Archives of Dermatological Research, Journal Year: 2025, Volume and Issue: 317(1)

Published: Feb. 14, 2025

Language: Английский

Citations

0
Inflammatory Transformation of Skin Basal Cells as a Key Driver of Cutaneous Aging DOI Open Access
Shupeng Liu,

Sheng Lu,

Zhiping P. Pang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2617 - 2617

Published: March 14, 2025

This study comprehensively investigated keratinocyte subpopulation heterogeneity and developmental trajectories during skin aging using single-cell sequencing, transcriptomics, facial aging-related genome-wide association studies (GWAS) data. We identified three major subpopulations: basal cells (BCs), spinous (SCs), IFI27+ keratinocytes. Single-cell pseudotime analysis revealed that can differentiate along two distinct paths: toward differentiation or the inflammatory state. With aging, proportion of significantly increased, displaying more active immunomodulatory signals. Through cell–cell communication analysis, we found signaling pathways, including NOTCH, PTPR, PERIOSTIN, exhibited characteristics different branches. Integration GWAS data significant loci on chromosomes 2, 3, 6, 9 were spatially correlated with key biological pathways (including antigen processing, oxidative stress, apoptosis). These findings reveal complex cellular molecular mechanisms underlying offering potential targets for novel diagnostic approaches therapeutic interventions.

Language: Английский

Citations

0
Discovery of Airway-Administered Ionophores for Mn2+ to Mitigate Lung Metastasis by Targeting Disseminated Tumor Cell DOI
Yuming Wang, Ni Fan,

Rui Wang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Activation of the STING pathway is essential for restoring immune surveillance against dormant disseminated tumor cells (DTCs) in lungs. Inhaled Mn2+ has potential as a agonist; however, its clinical application limited by risk chronic inflammation and metastasis, primarily due to reactive oxygen species (ROS) generation during inhalation. To address these risks, salvianolic acid B (salB) was identified an effective ionophore Mn2+, enhancing activation while mitigating ROS-induced inflammation. In this study, salB mitigated Mn2+-induced ROS levels enhanced signaling, providing safer, noninflammatory approach activating lung DTCs. The salB-Mn2+ complexes were encapsulated human serum albumin nanoparticles (HSA NPs) PET MRI analyses revealed that intratracheal administration HSA NP@salB-Mn2+ restricted Mn2+'s systemic distribution, retaining it lungs minimizing central nervous system accumulation. Subsequent immunofluorescence further confirmed effectively targeted metastatic lesions. Despite extended retention tissue, histological analysis showed minimal mice treated with NP@salB-Mn2+, contrast those receiving MnCl2 or MnO. Consequently, demonstrated superior suppression 4T1 cell metastasis postsurgical relative Mechanistically, functions agonist, independently p-STING, which synergizes significantly amplify signaling downstream target engagement. mouse model, combination αPD-1 antibody reduced DTC dormancy detection, confirming immunotherapeutic potential. These findings establish promising inhalable treatment, three key advantages: prolonged retention, risk, STING-activating efficacy.

Language: Английский

Citations

0
Integrated multidimensional spectra and multilevel data optimization for rapid origin tracing and quality assessment of Salvia miltiorrhiza DOI
Rao Fu, Chen Peng, Wenhao Dong

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113755 - 113755

Published: April 1, 2025

Language: Английский

Citations

0
Porphyromonas gingivalis induces Zbp1-mediated macrophages PANoptosis in periodonitis pathophysiology DOI Creative Commons
Jing Qin Wu, Zixiang Guo, Long Wang

et al.

Experimental & Molecular Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Periodontitis is an oral immunoinflammatory disease, and macrophages play a crucial role in its pathophysiology. However, macrophage death during antibacterial activities will exacerbate inflammation tissue damage. Porphyromonas gingivalis major constituent of subgingival biofilm plaques periodontitis, but the effects precise molecular mechanisms by which it triggers remain unknown. Here we found that P. infection notably activated multiple pathways bone-marrow-derived macrophages, including pyroptosis, apoptosis necrosis. Furthermore, using RNA sequencing, identified markedly increased expression Z-DNA binding protein 1 (Zbp1) macrophages. Initially as interferon-induced tumor-associated protein, Zbp1 serves upstream sensor regulates cell activating PANoptosis. Mechanistically, induced mitochondrial stress response, prompting release DNA. This DNA then interacted with Zbp1, consequently augmenting downstream PANoptosis signals. In addition, stimulated through Tlr2/4-JNK-Stat3/5 pathway, exacerbating death. Importantly, blocking biosynthesis endogenous pharmacological delivery microneedles improved survival gingivalis-infected inhibited periodontal destruction. These findings highlight potential therapeutic target for gingivalis-induced periodontitis.

Language: Английский

Citations

0
Role of Mitochondrial Dysfunction in UV‐Induced Photoaging and Skin Cancers DOI
Xinghua Yuan,

Huixin Li,

Ji Su Lee

et al.

Experimental Dermatology, Journal Year: 2025, Volume and Issue: 34(5)

Published: May 1, 2025

ABSTRACT Ultraviolet radiation (UVR) is the most detrimental external factor that induces acute photodamage, photoaging and skin cancers, with complex underlying molecular mechanisms initiated mainly by increased DNA damage reactive oxygen species (ROS) generation. Mitochondria are main organelles in cells produce ROS energy regulate various physiological pathological signalling pathways. Continuous UVR on human can induce mitochondrial mutations excessive production, creating feedback between each other subsequently causing a reduction membrane potential (MMP) respiratory capacity. Deficiencies function apoptosis, mitophagy senescence, resulting UVR‐induced photodamage photoaging. Mitochondrial biogenesis metabolic pathways play critical roles progression of particularly melanoma, which malignant infrequent type cancer. In this review, we describe recent advances determining intimate relationship damage, suggesting candidates novel chemical/natural components to protect from cancers via targeting mechanisms.

Language: Английский

Citations

0
Injectable Thermosensitive Gel Loaded Salvianolic Acid B Alleviates Periodontitis by Down‐Regulating Oxidative Stress and Protecting Mitochondrial Function DOI

Jiamin Hu,

Jingxin Zhao,

Shuhan Li

et al.

Polymers for Advanced Technologies, Journal Year: 2025, Volume and Issue: 36(5)

Published: May 1, 2025

ABSTRACT Periodontitis is an immune‐reactive disease characterized by excessive inflammation and oxidative stress (OS). Salvianolic acid B (Sal B) a component isolated from the root of Salvia miltiorrhiza , known for its strong anti‐inflammatory antioxidant effects. Our study examined beneficial effects Sal on OS mitochondrial function (MF) in periodontitis, as well potential mechanisms. In vitro experiments demonstrated that can reduce inflammatory response periodontal tissue cells eliminate products such reactive oxygen species (ROS). Furthermore, enhances membrane (MMP) increases adenosine triphosphate (ATP) production. We designed methacryloylated glycol chitosan/β‐glycerophosphate composite hydrogel (MAGC/β‐GP Gel) localized delivery B. B@MAGC/β‐GP gel stable exhibits good thermal sensitivity, allowing continuous release pocket. Results mouse model periodontitis showed significantly reduces alveolar bone absorption, eliminates ROS, MMP levels, alleviates inflammation. Overall, this demonstrates has therapeutic agent novel material expected to be used intrapocket injections clinical settings.

Language: Английский

Citations

0
Pilose Antler Protein Relieves UVB-Induced HaCaT Cells and Skin Damage DOI Creative Commons

Kaiyue Liu,

Chenxu Zhao,

Ke Zhang

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(17), P. 4060 - 4060

Published: Aug. 27, 2024

Extended exposure to UVB (280-315 nm) radiation results in oxidative damage and inflammation of the skin. Previous research has demonstrated that pilose antler extracts have strong anti-inflammatory properties possess antioxidant effects. This study aimed elucidate mechanism protein repairing photodamage caused by HaCaT cells ICR mice. Pilose (PAP) was found increase expression type I collagen hyaluronic acid under irradiation while also inhibiting reactive oxygen species (ROS) production stress vitro. In vivo, topical application effectively attenuated UVB-induced skin mice reducing interleukin-1β (IL-β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) alleviating stress. It shown repaired through MAPK TGF-β/Smad pathways.

Language: Английский

Citations

3