Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota DOI Creative Commons

Shulin Wei,

Mingxing Li, Long Zhao

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Dec. 23, 2024

Abstract Background Liuweizhiji Gegen-Sangshen oral liquid (LGS), as a Chinese medicinal preparation, is developed from Traditional formula consisting of six herbs, including Puerariae lobatae radix, Hoveniae semen, Imperatae rhizoma, Crataegi fructus, Mori fructus and Canarli has been extensively utilized in the prevention treatment alcoholic liver disease (ALD) clinically. Previous study demonstrated that LGS dose-dependently mitigated ALD rat models. However, whether how main characteristic constituents (the flavonoid polysaccharide fractions, LGSF LGSP) contribute to anti-ALD effect remains unclear. This aimed assess its fractions (LGSF murine model explore underlying mechanisms. Methods mouse was constructed using chronic binge ethanol feeding method. Biochemical determinations AST, ALT, TC, TG, ADH, ALDH, HDL, LDL, IL-1β, IL-6, TNF-α were performed corresponding kits. Histopathological examination intestinal sections conducted based on H&E staining. Lipid accumulation hepatocytes evaluated by oil red O Ethanol metabolism assessed determining activity ADH ALDH enzymes. Intestinal barrier function analyzed immunohistochemistry analysis ZO-1 occludin immunofluorescence epithelial markers, Lgr5, Muc2, Lyz1. apoptosis detected TUNEL Mouse fecal microbiota alterations 16S rRNA sequencing. An vitro injury established developing TNF-α-induced 3D-cultured organoids. In culture specific bacterial strains performed. Results The results showed significantly alleviated mice through attenuating hepatic inflammation, improving steatosis promoting metabolism. Notably, LGS, LGSP, damage maintained mice. protection generally more obvious than positive drug meltadosine. Further promoted repair via Lgr5 + stem cell mediated regeneration LGSF, other had better vitro. Moreover, remarkably gut dysbiosis at least partially recovery alcohol-induced microbial changes induction groups. indicated impact growth. but not growth Lactobacillus . Similarly, LGSP increased proliferation Bacteroides sartorii , minimal effect. all Bacillus coagulans Bifidobacterium adolescentis bifidum Dubosiella newyorkensis no Conclusions exerts regulating gut-liver axis, are responsible for protective

Language: Английский

Acanthopanax seeds polysaccharide improve alcoholic fatty liver disease through the gut-liver axis DOI

Liwen Qin,

Hongjie Piao,

Ci Song

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140200 - 140200

Published: Jan. 1, 2025

Language: Английский

Citations

0
Enhancing curcumin stability and bioavailability through chickpea protein isolate–citrus pectin conjugate emulsions: Targeted delivery and gut microecology modulation DOI
Yibo Liu, Xuemei Fan,

Xiyu Yu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 300, P. 140295 - 140295

Published: Jan. 23, 2025

Language: Английский

Citations

0
Genome-Wide Identification of the ARF Gene Family in Safflower (Carthamus tinctorius L.) and Their Response Patterns to Exogenous Hormone Treatments DOI Open Access

Shaomin Qin,

Xinrong Wen,

Mengyuan Ma

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3773 - 3773

Published: April 16, 2025

Auxin response factors (ARFs) are a class of transcription widely present in plants. As an important economic crop, research on the effects safflower ARFs endogenous auxin and effective components is relatively limited. In this study, total 23 ARF genes were identified from genome. Sequence alignment domain analysis indicated presence conserved B3 Auxin_resp domains these ARFs. Phylogenetic that CtARF could be classified into five subfamilies, conclusion also supported by gene structure, consensus motifs, compositions. Transcriptome data showed expressed all flower colors, but expression levels family members vary among different colors. CtARF19 had higher deep red flowers, CtARF3 white CtARF2/12 yellow CtARF21/22 light flowers. Protein–protein interaction network (CtARF2/3/4/5/15/18/19/22) located within network. Cis-acting element suggested may regulated hormone treatment (AuxRR-core) abiotic stress, results qRT-PCR confirmed this. Additionally, content active with colors significantly changed upon hormones affect IAA content. summary, our study provides valuable insights biological functions under exogenous conditions their components.

Language: Английский

Citations

0
Oxidative stress modulation in alcohol-related liver disease: From chinese botanical drugs to exercise-based interventions DOI Creative Commons
Yuting Zhu,

Yuqing Jia,

Enming Zhang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

Alcohol-related liver disease (ALD) is a chronic injury caused by long-term excessive alcohol consumption, with complex and multifaceted pathological mechanisms. Research indicates that oxidative stress (OS), inflammatory responses, lipid metabolic disturbances induced its metabolites are primary contributors to hepatocyte injury, positioning OS as key target in ALD treatment. The main non-pharmacological treatment for abstinence, while medical primarily relies on Western pharmacological interventions. However, recent research has increasingly highlighted the potential of Chinese botanical drugs improving histological features modulating signaling pathways associated ALD, underscoring therapeutic traditional herb medicine. Despite these promising findings, precise mechanisms effects extracts remain incompletely understood, side must be considered. Therefore, comprehensive analysis herbal essential optimize clinical administration ensure safe, effective This review focuses central theme, categorizing into six major groups-flavonoids, polyphenols, terpenoids, alkaloids, saponins, anthraquinones-all widely used provides an overview their characteristics actions context offering insights regulation exploring treatments ALD. Notably, physical exercise shares overlapping regulating OS. Combining two strategies could offer integrative though further needed confirm synergistic benefits applications.

Language: Английский

Citations

0
Phytochemicals Targeting Inflammatory Pathways in Alcohol-Induced Liver Disease: A Mechanistic Review DOI Creative Commons

Swati Tirunal Achary,

Prerna Gupta,

Ali H. Rajput

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(5), P. 710 - 710

Published: May 11, 2025

Alcoholic beverages play a significant role in social engagement worldwide. Excessive alcohol causes variety of health complications. Alcohol-induced liver disease (ALD) is responsible for the bulk linked fatalities. The activation immune mechanisms has crucial developing ALD. No effective medication promotes function, shields from harm, or aids hepatic cell regeneration. Alcohol withdrawal one most beneficial therapies ALD patients, which improves patient’s chances survival. There demand safe and reasonably priced approaches to treating it. Exploring naturally derived phytochemicals been fascinating path, it drawn attention recent years modulators inflammatory pathways prevention management In this review, we have discussed roles various ALD, highlighting importance intestinal barrier integrity gut microbiota, as well cells inflammation, other pathways, including cGAS-STING, NLRP3, MAPK, JAK-STAT, NF-kB. Further, review also outlines possible targeting these safeguard alcohol-induced injury. We highlighted that immunological using herbal medicine may find place counteract Preclinical vitro vivo investigations shown promising results; nonetheless, more extensive work required properly understand compounds’ action. Clinical are very transferring laboratory knowledge into patient therapy.

Language: Английский

Citations

0
Elucidation of the biosynthetic pathway of hydroxysafflor yellow A DOI Creative Commons
Zilong Wang,

H.C. Wang,

G.-K. Chang

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: May 14, 2025

Language: Английский

Citations

0
Mechanical mechanics-reclaiming a new battlefield for chronic liver disease DOI Creative Commons
Y Zhang, Tianle Ma, XingXing Lu

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Elucidating Hydroxysafflor yellow A's multi-target mechanisms against alcoholic liver disease through integrative pharmacology DOI
Haichuan Yu,

Qi-Rong Bai,

Jiao-Jie Guo

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 134, P. 155956 - 155956

Published: Aug. 15, 2024

Language: Английский

Citations

3
Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota DOI Creative Commons

Shulin Wei,

Mingxing Li, Long Zhao

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: Dec. 23, 2024

Abstract Background Liuweizhiji Gegen-Sangshen oral liquid (LGS), as a Chinese medicinal preparation, is developed from Traditional formula consisting of six herbs, including Puerariae lobatae radix, Hoveniae semen, Imperatae rhizoma, Crataegi fructus, Mori fructus and Canarli has been extensively utilized in the prevention treatment alcoholic liver disease (ALD) clinically. Previous study demonstrated that LGS dose-dependently mitigated ALD rat models. However, whether how main characteristic constituents (the flavonoid polysaccharide fractions, LGSF LGSP) contribute to anti-ALD effect remains unclear. This aimed assess its fractions (LGSF murine model explore underlying mechanisms. Methods mouse was constructed using chronic binge ethanol feeding method. Biochemical determinations AST, ALT, TC, TG, ADH, ALDH, HDL, LDL, IL-1β, IL-6, TNF-α were performed corresponding kits. Histopathological examination intestinal sections conducted based on H&E staining. Lipid accumulation hepatocytes evaluated by oil red O Ethanol metabolism assessed determining activity ADH ALDH enzymes. Intestinal barrier function analyzed immunohistochemistry analysis ZO-1 occludin immunofluorescence epithelial markers, Lgr5, Muc2, Lyz1. apoptosis detected TUNEL Mouse fecal microbiota alterations 16S rRNA sequencing. An vitro injury established developing TNF-α-induced 3D-cultured organoids. In culture specific bacterial strains performed. Results The results showed significantly alleviated mice through attenuating hepatic inflammation, improving steatosis promoting metabolism. Notably, LGS, LGSP, damage maintained mice. protection generally more obvious than positive drug meltadosine. Further promoted repair via Lgr5 + stem cell mediated regeneration LGSF, other had better vitro. Moreover, remarkably gut dysbiosis at least partially recovery alcohol-induced microbial changes induction groups. indicated impact growth. but not growth Lactobacillus . Similarly, LGSP increased proliferation Bacteroides sartorii , minimal effect. all Bacillus coagulans Bifidobacterium adolescentis bifidum Dubosiella newyorkensis no Conclusions exerts regulating gut-liver axis, are responsible for protective

Language: Английский

Citations

0