Multi-Omics on Traditional Medicinal Plant of the Genus Aconitum: Current Progress and Prospect DOI Creative Commons
Ting Wang,

Cai Rangji,

Wenbin Liu

et al.

Molecules, Journal Year: 2024, Volume and Issue: 30(1), P. 118 - 118

Published: Dec. 31, 2024

Aconitum stands out among the Ranunculaceae family for its notable use as an ornamental and medicinal plant. Diterpenoid alkaloids (DAs), characteristic compounds of Aconitum, have been found to effective analgesic anti-inflammatory effects. Despite their potential, toxicity most DAs restricts direct in traditional medicine, necessitating complex processing before use. The high-throughput omics allows investigation plant genetics, gene regulation, metabolic pathways, growth development. We collected comprehensive information on studies plants, encompassing genomics, transcriptomics, metabolomics, proteomics, microbiomics, from internationally recognized electronic scientific databases such Web Science, PubMed, CNKI. In light this, we identified research gaps proposed potential areas key objectives research, aiming establish a framework quality improvement, molecular breeding, deeper understanding specialized metabolite production plants.

Language: Английский

Biomod2 modeling for predicting the potential ecological distribution of three Fritillaria species under climate change DOI Creative Commons

Deya Huang,

Qiuju An,

Sipei Huang

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 1, 2023

Abstract The Fritillaria species ranked as a well-known traditional medicine in China and has become rare due to excessive harvesting. To find reasonable strategy for conservation cultivation, identification of new ecological distribution together with prediction those responses climate change are necessary. In terms current occurrence records bioclimatic variables, the suitable habitats delavayi , taipaiensis wabuensis were predicted. comparison Maxent GARP, Biomod2 obtained best AUC, KAPPA TSS values larger than 0.926 was chosen construct model. Temperature seasonality indicated put greatest influence on while isothermality most importance . areas three distributed south-west China, accounting approximately 17.72%, 23.06% 20.60% China's total area, respectively. During 2021–2100 period, F. reached maximum under SSP585 scenario, that SSP126 scenario. high niche overlap among showed correlation chemical composition ( P ≤ 0.05), no observed between DNA barcodes, indicating spatial had major species. Finally, acquisition species-specific would contribute decrease habitat competition, future cultivation

Language: Английский

Citations

25
UDP‐glucosyltransferases from UGT73 family catalyze 3‐O‐glucosylation of isosteroidal and steroidal alkaloids in Fritillaria unibracteata var. wabuensis DOI Open Access
Jingjin Wang, Zikun Wang,

Hsihua Wang

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(5)

Published: March 1, 2025

SUMMARY Fritillaria unibracteata var. wabuensis is an important resource plant for the famous traditional Chinese medicine Fritillariae cirrhosae bulbus (“Chuanbeimu” in Chinese). F. dried bulbs of several species from genus, with isosteroidal alkaloids components assumed as bioactive ingredients. However, biosynthesis pathway remains elusive. Here, we adopted a material to identify genes involved alkaloids. We first constructed multi‐tissue metabolome and transcriptome dataset . Interestingly, imperialine‐3‐β‐ d ‐glucoside, glycoalkaloid, was found be major tissue‐specific accumulated alkaloid. Through phylogenetic co‐expression analysis, identified two UDP‐glucosyltransferases UGT73 family catalyzing 3‐ O ‐glucosylation steroidal alkaloids: imperialine ‐glucosyltransferase (FuwI3GT) can use both alkaloid solanidine substrates, while (FuwS3GT) only substrate. further approved that W201 residue FuwI3GT determined its substrate preference Overall, our results enzymes paved way fully elucidate species.

Language: Английский

Citations

1
Bioinformatic Analysis of Codon Usage Bias of HSP20 Genes in Four Cruciferous Species DOI Creative Commons
Huiyue Ji,

J.L. Liu,

Yineng Chen

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(4), P. 468 - 468

Published: Feb. 6, 2024

Heat shock protein 20 (HSP20) serves as a chaperone and plays roles in numerous biological processes, but the codon usage bias (CUB) of its genes has remained unexplored. This study identified 140 HSP20 from four cruciferous species, Arabidopsis thaliana, Brassica napus, rapa, Camelina sativa, that were Ensembl plants database, we subsequently investigated their CUB. As result, base composition analysis revealed overall GC content was below 50%. The significantly correlated with constituents at three positions, implying both mutation pressure natural selection might contribute to relatively high ENc values suggested CUB species weak. Subsequently, exhibited negative correlation gene expression levels. Analyses, including ENc-plot analysis, neutral PR2 bias, mainly shaped patterns these species. In addition, total 12 optimal codons (ΔRSCU > 0.08 RSCU 1) across A neighbor-joining phylogenetic based on coding sequences (CDS) showed strictly distinctly clustered into subfamilies. Principal component cluster relative synonymous (RSCU) supported fact pattern consistent genetic relationship level (or) These results will not only enrich resource also advance our understanding genes, which may underlie theoretical basis for exploration evolutionary pattern.

Language: Английский

Citations

5
Botanical characterization, phytochemistry, biosynthesis, pharmacology clinical application, and breeding techniques of the Chinese herbal medicine Fritillaria unibracteata DOI Creative Commons
Yamei Zhang,

Hongping Han,

Dingai Li

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: July 9, 2024

Fritillaria unibracteata (FRU) belongs to the genus of Liliaceae family. It is one original plants Chinese medicinal material “Chuanbeimu” and also a biological resource featured in Tibetan Plateau China. The dried bulbs FRU are used traditional medicine. chemical constituents that have been isolated identified include alkaloids, sterols, organic acids their esters, nucleosides volatile oils. has antitussive, expectorant, anti-asthmatic, anti-inflammatory, antibacterial, acute lung injury-reducing, antifibrosis, antitumor, other pharmacological effects. This valuable plant an extremely high market demand, over years, due over-exploitation, now listed as key species endangered scarcely cultivated China herb. However, research on rare, its effective components, control, mechanisms action need further study. review systematically discusses herbal characteristics, distribution, composition, biosynthesis, effects, clinical application, breeding techniques FRU, hoping provide reference for use FRU.

Language: Английский

Citations

4
Spatial distribution of alkaloids in Fritillaria Bulbus using UPLC-Q-Exactive Orbitrap MS/MS and desorption electrospray MS imaging DOI Creative Commons

Xichuang Guo,

Keyun Cheng, Chenxin Wang

et al.

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117544 - 117544

Published: Feb. 1, 2025

Language: Английский

Citations

0
LED blue light enhances the accumulation and synthesis of steroidal alkaloids in Fritillaria unibracteata Hsiao et K. C. Hsia in vitro DOI

Maoyao Peng,

Zhiqi Liu, Xiaohong Chen

et al.

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 216, P. 118836 - 118836

Published: May 27, 2024

Language: Английский

Citations

3
Differential roles of Cassia tora 1-deoxy-D-xylulose-5-phosphate synthase and 1-deoxy-D-xylulose-5-phosphate reductoisomerase in trade-off between plant growth and drought tolerance DOI Creative Commons

Chunyao Tian,

Huige Quan,

Ruiping Jiang

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 20, 2023

Due to global climate change, drought is emerging as a major threat plant growth and agricultural productivity. Abscisic acid (ABA) has been implicated in tolerance, however, its retarding effects on cannot be ignored. The reactions catalyzed by 1-deoxy-D-xylulose-5-phosphate synthase (DXS) reductoisomerase (DXR) proteins are critical steps within the isoprenoid biosynthesis plants. Here, five DXS (CtDXS1-5) two DXR (CtDXR1-2) genes were identified from Cassia tora genome. Based multiple assays including phylogeny, cis-acting element, expression pattern, subcellular localization, CtDXS1 CtDXR1 might potential candidates controlling biosynthesis. Intriguingly, transgenic plants resulted tolerance but retardant growth, while exhibited both enhanced increased growth. By comparison of β-carotene, chlorophyll, abscisic gibberellin 3 (GA3) contents wild-type plants, absolute (or) altered GA3/ABA levels suggested responsible for balance between transcriptome that transcript key genes, such DXS, 9-cis-epoxycarotenoid dioxygenases (NCED), ent-kaurene (KS) etc, involved with ABA GA3 induced their accordingly. Collectively, trade-off effect was associated redesigning architecture phytohormone homeostasis thus highlighted future breeding purposes.

Language: Английский

Citations

7
Genome-wide identification of AP2/ERF gene family in Coptis Chinensis Franch reveals its role in tissue-specific accumulation of benzylisoquinoline alkaloids DOI Creative Commons

Mengyu Zhang,

Pingping Lu,

Yating Zheng

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 16, 2024

The Plant-specific AP2/ERF gene family encodes proteins involved in various biological and physiological processes. Although the genome of Coptis chinensis Franch, a plant producing benzylisoquinoline alkaloids (BIAs), has been sequenced at chromosome level, studies on C. are lacking. Thus, genome-wide identification was conducted to explore its role BIAs biosynthesis.

Language: Английский

Citations

2
Metabolomic and transcriptomic analyses reveals candidate genes and pathways involved in secondary metabolism in Bergenia purpurascens DOI Creative Commons
Qiankun Zhu, Yufeng Wu, Xuebin Zhang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Nov. 14, 2024

Bergenia purpurascens is an important medicinal, edible and ornamental plant. The lack of omics information hinders the study its metabolic pathways related genes. In order to investigate candidate genes involved in secondary metabolism B. purpurascens, roots, stems leaves were subjected metabolomic transcriptomic analyses this study. A total 351 differentially accumulated metabolites identified. We identified 120 phenylpropanoid flavonoid biosynthesis pathway, from which 29 key obtained by WGCNA. Five UDP-Glycosyltransferases four O-methyltransferases suggested be enzymes synthetic pathway gallic acid bergenin correlation analysis between transcriptional levels phylogenetic analysis. This provides data resources new insights for further studies on major active components purpurascens.

Language: Английский

Citations

2
In vitro propagation and omics breakthroughs for understanding specialized metabolite production in high-value Himalayan Fritillaria species DOI
Shagun Sharma, Pankaj Kumar, Rohit Sharma

et al.

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 205, P. 117541 - 117541

Published: Sept. 30, 2023

Language: Английский

Citations

6