Single-cell sequencing provides clues about the developmental genetic basis of evolutionary adaptations in syngnathid fishes

eLife, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 3, 2025

Seahorses, pipefishes, and seadragons are fishes from the family Syngnathidae that have evolved extraordinary traits including male pregnancy, elongated snouts, loss of teeth, dermal bony armor. The developmental genetic cellular changes led to evolution these largely unknown. Recent syngnathid genome assemblies revealed suggestive gene content differences provided opportunity for detailed analyses. We created a single-cell RNA sequencing atlas Gulf pipefish embryos understand basis four traits: derived head shape, toothlessness, armor, pregnancy. completed marker analyses, built networks, examined spatial expression select genes. identified osteochondrogenic mesenchymal cells in elongating face express regulatory genes bmp4, sfrp1a , prdm16 . found no evidence tooth primordia cells, we observed re-deployment osteoblast networks developing Finally, epidermal expressed nutrient processing environmental sensing genes, potentially relevant brooding environment. evolutionary innovations composed recognizable cell types, suggesting features originate within existing networks. Future work addressing across multiple stages species is essential understanding how novelties fish evolved.

Language: Английский

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Seahorse Male Pregnancy as a Model System to Study Pregnancy, Immune Adaptations, and Environmental Effects

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9712 - 9712

Published: June 3, 2023

Seahorses, together with sea dragons and pipefishes, belong to the

Language: Английский

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The detoxification ability of sex-role reversed seahorses determines the sexual dimorphism in immune responses to benzo[a]pyrene exposure

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 933, P. 173088 - 173088

Published: May 11, 2024

Language: Английский

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Defence against the intergenerational cost of reproduction in males: oxidative shielding of the germline

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2023, Volume and Issue: 99(1), P. 70 - 84

Published: Sept. 12, 2023

ABSTRACT Reproduction is expected to carry an oxidative cost, yet in many species breeders appear sustain lower levels of damage compared non‐breeders. This paradox may be explained by considering the intergenerational costs reproduction. Specifically, a reduction upon transitioning reproductive state represent pre‐emptive shielding strategy protect next generation from (IOD) – known as hypothesis. Males particularly likely transmit IOD, because sperm are highly susceptible damage. Yet, possibility male‐mediated IOD remains largely uninvestigated. Here, we present conceptual and methodological framework assess reproduction germline males. We discuss variance payoffs according species' life histories, impact on offspring fitness. Oxidative presents opportunity incorporate effects into advancing field life‐history evolution.

Language: Английский

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Single Cell RNA Sequencing Provides Clues for the Developmental Genetic Basis of Syngnathidae’s Evolutionary Adaptations

Published: June 5, 2024

Seahorses, pipefishes, and seadragons are fishes from the family Syngnathidae that have evolved extraordinary traits including male pregnancy, elongated snouts, loss of teeth, dermal bony armor. The developmental genetic cellular changes led to evolution these largely unknown. Recent syngnathid genomes revealed suggestive gene content differences provide opportunity for detailed analyses. We created a single cell RNA sequencing atlas Gulf pipefish embryos understand basis four traits: derived head shape, toothlessness, armor, pregnancy. completed marker analyses, built networks, examined spatial expression select genes. identified osteochondrogenic mesenchymal cells in elongating face express regulatory genes bmp4, sfrp1a , prdm16 . found no evidence tooth primordia cells, we observed re-deployment osteoblast networks developing armor.Finally, epidermal expressed nutrient processing environmental sensing genes, potentially relevant brooding environment. evolutionary innovations composed recognizable types, suggesting features originate within existing networks. Future work addressing across multiple stages species is essential understanding how their novelties evolved.

Language: Английский

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Androgens and androgen receptor directly induce the thickening, folding, and vascularization of the seahorse abdominal dermal layer into a placenta-like structure responsible for male pregnancy via multiple signaling pathways

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 279, P. 135039 - 135039

Published: Aug. 27, 2024

Language: Английский

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Gonad and Germ Cell Development and Maturation Characteristics of the Pot-Bellied Seahorse (Hippocampus abdominalis) under Captive Breeding Conditions in Northern China

Fishes, Journal Year: 2023, Volume and Issue: 8(11), P. 551 - 551

Published: Nov. 15, 2023

Ovoviviparity and male pregnancy represent distinctive reproductive strategies in seahorses. However, the detailed process of gonadal development seahorses, particularly pot-bellied seahorse (Hippocampus abdominalis), remains largely unknown. In this study, we investigated complete under captive breeding conditions (18 ± 1 °C). Immediately after birth, primordial germ cells (PGCs) were found within genital ridge, enclosed by a single layer somatic cells. Around 7–9 days birth (DAB), ovary begins to differentiate. By 30 DAB, two germinal ridges had formed along edge follicular lamina ovary. The primary oocytes, resulting from differentiation process, gradually migrated dorsal sides mid-ventral area ovary, eventually maturing into eggs. testis, secondary spermatocytes appeared at 15 respectively, preceding formation testicular lumen (50 DAB). testis was observed consist large compartment. Under Northern China, demonstrated year-round capability, with each producing approximately 100–150 larvae. findings study contribute valuable insights aquaculture enhance understanding unique strategy employed

Language: Английский

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Evolutionary traits and functional roles of chemokines and their receptors in the male pregnancy of the Syngnathidae

Marine Life Science & Technology, Journal Year: 2023, Volume and Issue: 5(4), P. 500 - 510

Published: Nov. 22, 2023

Language: Английский

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Seahorse brood pouch morphology and control of male parturition in Hippocampus abdominalis

Placenta, Journal Year: 2022, Volume and Issue: 127, P. 88 - 94

Published: Aug. 6, 2022

Language: Английский

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Parent-specific transgenerational immune priming enhances offspring defense - unless heat-stress negates it all

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 29, 2024

Abstract Trans-generational immune priming (TGIP) adjusts offspring responses based on parental immunological experiences - a form of trans-generational plasticity predicted to be adaptive when parent-offspring environmental conditions match. In contrast, mis-matches between negate those advantages, rendering TGIP costly mismatched phenotypes are induced. Particularly maternal was thought shape preparedness: mothers’ eggs contain more substance than sperm and, in viviparous species, pregnancy provides additional avenues for developing offspring. The syngnathids’ (pipefishes and seahorses) unique male an unusual perspective the ecological relevance system where egg production occur different sexes. We simulated bacteria exposure broad nosed pipefish, Syngnathus typhle , through vaccinations with heat-killed Vibrio aestuarianus before mating fish each other or control individuals. Resulting were raised, some exposed V. heat-stress environment, after which transcriptome microbiome compositions investigated. Transcriptomic effects only observed -exposed at temperatures, arguing low costs non-matching environments. elicited by paternal had limited overlap not additive. Both transcriptomic significantly associated functions, specifically response innate branch. both parents reduced relative abundance experimental offspring, showcasing its effectiveness. Despite significance matching biotic environments, no TGIP-associated heat-treated Heat-spikes caused climate change thus threaten benefits, potentially increasing susceptibility emerging marine diseases. This highlights urgent need understand how animals will cope climate-induced changes microbial assemblages illustrating importance – limits mitigating impacts stressors vulnerability.

Language: Английский

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