Advances in the study of the function and mechanism of the action of flavonoids in plants under environmental stresses

Planta, Journal Year: 2023, Volume and Issue: 257(6)

Published: May 3, 2023

Language: Английский

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The Role of Polyphenols in Abiotic Stress Tolerance and Their Antioxidant Properties to Scavenge Reactive Oxygen Species and Free Radicals

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 74 - 74

Published: Jan. 10, 2025

Plants have evolved complex mechanisms to cope with diverse abiotic stresses, the phenylpropanoid pathway playing a central role in stress adaptation. This produces an array of secondary metabolites, particularly polyphenols, which serve multiple functions plant growth, development, regulating cellular processes, and responses. Recent advances understanding molecular underlying metabolism revealed regulatory networks involving MYB transcription factors as master regulators their interactions signaling pathways. review summarizes our current polyphenol-mediated adaptations plants, emphasizing regulation function key compounds. We discussed how various including heat chilling stress, drought, salinity, light UV radiation, nanoparticles chemical heavy metal toxicity, modulate trigger accumulation specific polyphenolic The antioxidant properties these phenolic acids, flavonoids, anthocyanins, lignin, roles reactive oxygen species scavenging, neutralizing free radicals, membrane stabilization, osmotic adjustment are discussed. Understanding metabolic responses is crucial for developing stress-resilient crops improving agricultural productivity under increasingly challenging environmental conditions. provides comprehensive insights into integrating adaptation mechanisms, highlighting potential targets enhancing crop tolerance through adjustment.

Language: Английский

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Genome-wide analysis of UDP-glycosyltransferases family and identification of UGT genes involved in abiotic stress and flavonol biosynthesis in Nicotiana tabacum

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: April 19, 2023

Uridine disphosphate (UDP) glycosyltransferases (UGTs) act upon a huge variety of highly diverse and complex substrates, such as phytohormones specialized metabolites, to regulate plant growth, development, disease resistance, environmental interactions. However, comprehensive investigation UGT genes in tobacco has not been conducted.In this study, we carried out genome-wide analysis family-1 UDP Nicotiana tabacum. We predicted 276 NtUGT genes, which were classified into 18 major phylogenetic subgroups. The invariably distributed among all the 24 chromosomes with structural diversity exon/intron structure, conserved motifs, cis-acting elements promoters. Three groups proteins involved flavonoid biosynthesis, growth transportation modification identified that interact using PPI analysis. Expression cold stress, drought stress different flower color both online RNA-Seq data realtime PCR analysis, suggested distinct role resistance cold, biosynthesis. enzymatic activities seven potentially glycosylation analyzed, found exhibited activity on myricetin; six (NtUGT108, NtUGT123, NtUGT141, NtUGT155, NtUGT179, NtUGT195) showed cyanidin; three NtUGT195, NtUGT217) active flavonol aglycones kaempferol quercetin, catalyzing substrates (myricetin, cyanidin or flavonol) form new products. further investigated products properties NtUGT108, NtUGT217, their toward flavonol, NtUGT217 highest catalyzed efficient quercetin. Overexpression significantly increase content levels quercetin-3-O-glucoside, quercetin-3-O-rutinoside kaempferol-3-O-rutinoside transgenic leaves.We Our study uncovered valuable information about distribution, genomic characters, expression patterns tobacco. overexpressed validate its function catalyze results provide key candidate for future breeding potential metabolic engineering compounds.

Language: Английский

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Genome-wide analysis of UDP-glycosyltransferase gene family and identification of a flavonoid 7-O-UGT (AhUGT75A) enhancing abiotic stress in peanut (Arachis hypogaea L.)

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Dec. 7, 2023

Glycosylation, catalyzed by UDP-glycosyltransferase (UGT), was important for enhancing solubility, bioactivity, and diversity of flavonoids. Peanut (Arachis hypogaea L.) is an oilseed cash crop worldwide. In addition to provide high quality edible oils proteins, peanut seeds contain a rich source flavonoid glycosides that benefit human health. However, information UGT gene family quite limited in peanut.In present study, total 267 AhUGTs clustered into 15 phylogenetic groups were identified genome. Group I has greatly expanded the largest number AhUGT genes. Segmental duplication major driving force expansion. Transcriptomic analysis expression profiles various tissues under different abiotic stress treatments indicated involved growth response. AhUGT75A (UGT73CG33), located mitochondria, characterized as 7-O-UGT vitro enzyme assays. The transcript level strongly induced stress. Overexpression resulted accumulating less amount malondialdehyde (MDA) superoxide, tolerance against drought and/or salt transgenic Arabidopsis. These results played roles conferring through reactive oxygen species scavenging.Our research only not provides valuable functional characterization UGTs peanut, but also gives new insights potential applications breeding cultivars with both desirable health benefits.

Language: Английский

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Designing plant flavonoids: harnessing transcriptional regulation and enzyme variation to enhance yield and diversity

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: July 28, 2023

Plant synthetic biology has emerged as a powerful and promising approach to enhance the production of value-added metabolites in plants. Flavonoids, class plant secondary metabolites, offer numerous health benefits have attracted attention for their potential use plant-based products. However, achieving high yields specific flavonoids remains challenging due complex diverse metabolic pathways involved biosynthesis. In recent years, approaches leveraging transcription factors enzyme diversity demonstrated promise enhancing flavonoid expanding repertoire. This review delves into latest research progress engineering, encompassing identification manipulation enzymes biosynthesis, well deployment tools designing pathways. underscores importance employing carefully-selected boost harnessing promiscuity broaden or streamline biosynthetic steps required effective engineering. By power deeper understanding future researchers can potentially transform landscape product development across food beverage, pharmaceutical, cosmetic industries, ultimately benefiting consumers worldwide.

Language: Английский

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Overexpressing of GT8 confers resistance to fenoxaprop‐P‐ethyl in Alopecurus japonicus

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

Alopecurus japonicus is one of the most predominant weeds in wheat fields across China, where significant herbicide resistance has emerged over past decade. When compared to susceptible (S) population, resistant (R) population exhibited a 9.48-fold increase fenoxaprop-P-ethyl. The R displayed cross-resistance haloxyfop-P-methyl, quizalofop-P-ethyl, clodinafop-propargyl, sethoxydim, clethodim and pinoxaden. No known mutations or overexpression ACCase were detected population. showed enhanced metabolism fenoxaprop-P-ethyl, as evidenced by high-performance liquid chromatography analysis. cytochrome P450 (CYP450) inhibitor malathion glutathione-S-transferase (GST) 4-chloro-7-nitrobenzoxadiazole (NBD-Cl) partially reversed fenoxaprop-P-ethyl Six upregulated genes identified via RNA-sequencing, including two CYP450 (CYP86B1 CYP71C1), GST gene (GSTT1) three glycosyl transferase (GT) (UGT73C, GT8 CGT). Specifically, expression yeast decreased sensitivity suggesting its potential involvement metabolism. Molecular docking analysis further suggests that may be involved Our findings not only responsible for A. but also provide valuable resource crop genetic engineering. These insights could inform development effective management strategies japonicus. © 2025 Society Chemical Industry.

Language: Английский

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Four glycosyltransferase genes are responsible for synthesis and accumulation of different flavonol glycosides in apple tissues

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(4), P. 1937 - 1952

Published: June 25, 2024

SUMMARY Flavonols are widely synthesized throughout the plant kingdom, playing essential roles in physiology and providing unique health benefits for humans. Their glycosylation plays significant role improving their stability solubility, thus accumulation function. However, genes encoding enzymes catalyze this remain largely unknown apple. This study utilized a combination of methods to identify such enzymes. Initially, candidate were selected based on potential encode UDP‐dependent glycosyltransferases (UGTs) expression patterns response light induction. Subsequently, through testing vitro enzyme activity proteins produced Escherichia coli cells, four candidates confirmed flavonol 3‐ O ‐galactosyltransferase (UGT78T6), ‐glucosyltransferase (UGT78S1), ‐xylosyltransferase/arabinosyltransferase (UGT78T5), ‐rhamnosyltransferase (UGT76AE22), respectively. Further validation these genes' functions was conducted by modulating levels stably transformed apple plants. As anticipated, positive correlation observed between content specific glycosides corresponding each gene. Moreover, overexpression synthase gene, MdFLS , resulted increased glycoside roots leaves. These findings provide valuable insights breeding programs aimed at enriching flesh with flavonols identifying ‐glycosyltransferases other species.

Language: Английский

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Genome-Wide Identification of the Uridine Diphosphate Glucotransferase Gene Family and Expression Profiling Analysis in the Stem Development of Prunus mume

Forests, Journal Year: 2025, Volume and Issue: 16(1), P. 131 - 131

Published: Jan. 12, 2025

Prunus mume, a traditional ornamental species native to China, is highly valued for both its captivating weeping variety and economic value. The glycosylation of metabolites, which mediated by UDP-glycosyltransferases (UGTs), essential the regulation secondary metabolic pathways in plants. Here, we systematically identified analyzed UGTs P. mume. A total 182 PmUGTs were using genomic data categorized into 16 distinct subfamilies (A–P). All distributed unevenly across eight chromosomes, with clear evidence tandem duplication. Additionally, synteny analysis revealed close evolutionary relationship between mume persica. promoter cis-acting element indicated that may respond light, hormones, external stresses. heatmap had specific expression patterns different tissues, under various hormone treatments, developmental stages stem lignification. Notably, qRT-PCR verification showed significant differences PmUGT163 straight stems, underscoring role regulating plant architecture formation. Taken together, our study elucidates trajectory lays groundwork further validation candidate genes involved architectural

Language: Английский

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Functional Characterization of a Highly Efficient UDP-Glucosyltransferase CitUGT72AZ4 Involved in the Biosynthesis of Flavonoid Glycosides in Citrus

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: 73(9), P. 5450 - 5464

Published: Feb. 18, 2025

Citrus is an important dietary source of flavonoid glycosides, and UDP-glycosyltransferases (UGTs) are the key enzymes responsible for their glycosylation. In this study, a genome-wide analysis CitUGT gene family was conducted to identify CitUGTs that contribute 4′-O-glucosides biosynthesis. Our identified 136 in clementina genome, classifying them into 18 phylogenetic groups (A–R) 25 families. This classification strongly supported by consistent structures motif patterns. Moreover, we (Ciclev10025462m, designated CitUGT72AZ4) encodes 4′-O-glucosyltransferase first time citrus. enzyme preferentially glycosylated 4′-OH group multiple flavonoids, exhibiting higher catalytic efficiency toward quercetin three flavones vitro. Virus-induced silencing CitUGT72AZ4 significantly decreased accumulation 4′-O-glucosides. These results indicated participated biosynthesis 4′-O-glucoside Overall, our findings provide valuable insights its functional characterization.

Language: Английский

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Identification and characteristic analysis of PavUGT48 as a novel UDP-glycosyltransferase with dual functions on anthocyanin and amygdalin biosynthesis in sweet cherry

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143062 - 143062

Published: April 1, 2025

Language: Английский

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