Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis

Antioxidants, Journal Year: 2023, Volume and Issue: 12(11), P. 2014 - 2014

Published: Nov. 17, 2023

Plants are exposed to a variety of abiotic and biotic stresses leading increased formation reactive oxygen species (ROS) in plant cells. ROS capable oxidizing proteins, pigments, lipids, nucleic acids, other cell molecules, disrupting their functional activity. During the process evolution, numerous antioxidant systems were formed plants, including enzymes low molecular weight non-enzymatic antioxidants. Antioxidant perform neutralization therefore prevent oxidative damage components. In present review, we focus on biosynthesis antioxidants higher plants cells such as ascorbic acid (vitamin C), glutathione, flavonoids, isoprenoids, carotenoids, tocopherol E), ubiquinone, plastoquinone. Their functioning reactivity with respect individual will be described. This review is also devoted modern genetic engineering methods, which widely used change quantitative qualitative content cultivated plants. These methods allow various lines given properties obtained rather short time. The most successful approaches for transgenesis genome editing enhancement these discussed.

Language: Английский

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Anthocyanins: a comprehensive review on biosynthesis, structural diversity, and industrial applications

Phytochemistry Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: April 5, 2024

Language: Английский

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Application of Genome Editing Technologies to Improve Grain Quality of Pulses

Published: Jan. 1, 2025

Language: Английский

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Genome Editing for Horticultural Crop Improvement: Applications, Challenges, and Future Perspectives

Published: Jan. 1, 2025

Language: Английский

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Genetic mechanisms, biological function, and biotechnological advance in sorghum tannins research

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108573 - 108573

Published: March 1, 2025

Language: Английский

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Enhancing Horticultural Crops through Genome Editing: Applications, Benefits, and Considerations

Horticulturae, Journal Year: 2023, Volume and Issue: 9(8), P. 884 - 884

Published: Aug. 3, 2023

Genome editing has emerged as a powerful tool for accelerating crop improvement in horticultural crops by enabling precise modifications to their genetic makeup. This review provides an in-depth exploration of the applications, methodologies, and potential impacts genome horticulture. The focuses on three major tools horticulture, CRISPR-Cas9, TALENs, ZFNs. underlying mechanisms, challenges associated with each are discussed detail. being versatile widely used system, enhance traits such disease resistance, abiotic stress tolerance, nutritional content, yield crops. TALENs ZFNs, although less commonly used, offer alternative options targeted DNA modifications, have demonstrated success specific applications. We emphasize benefits including improved productivity, quality, value. However, off-target effects, delivery methods, regulatory frameworks need be addressed full realization this technology’s potential. serves valuable resource researchers, policymakers, stakeholders, providing insights into opportunities complexities harnessing enhanced By navigating these challenges, can contribute sustainable advancements benefiting both producers consumers worldwide.

Language: Английский

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Integrative HPLC profiling and transcriptome analysis revealed insights into anthocyanin accumulation and key genes at three developmental stages of black rice (Oryza sativa. L) caryopsis

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 30, 2023

Introduction Anthocyanins are plants' secondary metabolites belonging to the flavonoid class with potential health-promoting properties. They greatly employed in food industry as natural alternative colorants for dairy and ready-to-eat desserts pH indicators. These tremendous advantages make them economically important increasing market trends. Black rice is a rich source of anthocyanin that can be used ensure nutritional security around world. However, research on accumulation gene expression during caryopsis development lacking. Methods In this study, we combined high-performance liquid chromatography (HPLC) transcriptome analysis profile changes content dynamics at three developmental stages (milky, doughy, mature). Results Our results showed started visible seven days after flowering (DAF), increased rapidly from milky (11 DAF) dough stage, then decreasing peak was attained 18 DAF. RNA-seq 519 out 14889, 477 17914, 1614 18810 genes were uniquely expressed milky, mature stages, respectively. We performed pairwise comparisons: vs. dough, mature, identified 6753, 9540, 2531 DEGs, The DEGs' abundance higher 5527 up-regulated 4013 down-regulated genes, while it smaller 1419 1112 DEGs. This result consistent profiling, structural, regulatory, transporter involved biosynthesis their highest stage. Through protein interaction network, deciphered six main contributors observed including Os ANS, OsDFR, OsGSTU34, OsMYB3, OsbHLH015 , WD40-50 . Discussion study first report investigation black caryopsis. findings could aid predicting best harvesting time achieve maximum collect samples various analysis, laying groundwork future into molecular mechanisms underlying coloration.

Language: Английский

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Optimization of CRISPR/Cas9 ribonucleoprotein delivery into cabbage protoplasts for efficient DNA-free gene editing

Plant Biotechnology Reports, Journal Year: 2024, Volume and Issue: 18(3), P. 415 - 424

Published: April 10, 2024

Language: Английский

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Enhancing anthocyanin extraction efficiency in vegetables and fruits: a high‐speed shear homogenization technology

Journal of the Science of Food and Agriculture, Journal Year: 2024, Volume and Issue: 104(14), P. 8975 - 8990

Published: July 11, 2024

To extract anthocyanins with high efficiency, a hypothesis for high-speed shear homogenization extraction (HSHE) method was established through combination of solvent and ultrasonic-assisted extractions. The efficacy this demonstrated by performing qualitative quantitative analyses 16 extracted from five northern vegetables, berry fruits using ultra-high-performance Q-Exactive Orbitrap tandem mass spectrometry. Single-factor experiments were conducted varying ethanol concentration, temperature, pH cycles to determine the optimal conditions method.

Language: Английский

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Development and application of gene editing in citrus: how and what to do

Horticulture Advances, Journal Year: 2024, Volume and Issue: 2(1)

Published: Dec. 10, 2024

Abstract Conventional breeding techniques have been effectively utilized for the enhancement of citrus varieties. Nonetheless, traits such as an extended juvenile phase, cross- or self-incompatibility, high genetic heterozygosity, and polyembryony posed significant challenges limitations to these methods. The clustered regularly interspaced short palindromic repeats (CRISPR) genome editing has progressively emerged a vital tool research. This article reviews array CRISPR/Cas systems, emphasizes recent advancements in using CRISPR/Cas, explores application this technology bolster resistance canker. review also covers development CRISPR/Cas-mediated transformation regeneration systems citrus, alongside approaches generating transgene-free germplasm. Moreover, regulatory landscape societal acceptance are examined. Lastly, potential applications proposed, with attention prospective challenges.

Language: Английский

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