Current Progress, Applications and Challenges of Multi-Omics Approaches in Sesame Genetic Improvement

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3105 - 3105

Published: Feb. 4, 2023

Sesame is one of the important traditional oil crops in world, and has high economic nutritional value. Recently, due to novel throughput sequencing techniques bioinformatical methods, study genomics, methylomics, transcriptomics, proteomics metabonomics sesame developed rapidly. Thus far, genomes five accessions have been released, including white black seed sesame. The genome studies reveal function structure genome, facilitate exploitation molecular markers, construction genetic maps pan-genomes. Methylomics focus on level changes under different environmental conditions. Transcriptomics provide a powerful tool abiotic/biotic stress, organ development, noncoding RNAs, also some support studying abiotic stress traits. In addition, opportunities challenges multi-omics genetics breeding were described. This review summarizes current research status from perspectives hopes help for further in-depth

Language: Английский

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Biochemical and Molecular Insights into Variation in Sesame Seed Antioxidant Capability as Revealed by Metabolomics and Transcriptomics Analysis

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 514 - 514

Published: April 25, 2024

Sesame seeds are important resources for relieving oxidation stress-related diseases. Although a significant variation in seeds’ antioxidant capability is observed, the underlying biochemical and molecular basis remains elusive. Thus, this study aimed to reveal major seed components key mechanisms that drive variability of activity (AOA) using panel 400 sesame accessions. The AOA, total flavonoid, phenolic contents varied from 2.03 78.5%, 0.072 3.104 mg CAE/g, 2.717 21.98 GAE/g, respectively. Analyses revealed flavonoids acids main contributors AOA variation, irrespective coat color. LC-MS-based polyphenol profiling high (HA) low (LA) uncovered 320 differentially accumulated compounds (DAPs), including 311 up-regulated HA seeds. Tricin, persicoside, 5,7,4′,5′-tetrahydro-3′,6-dimethoxyflavone, 8-methoxyapigenin, 6,7,8-tetrahydroxy-5-methoxyflavone were top five HA. Comparative transcriptome analysis at three developmental stages identified 627~2357 DEGs unveiled differential regulation flavonoid biosynthesis, phenylpropanoid stilbene biosynthesis capacity variation. Major regulated structural genes transcription factors identified. SINPZ0000571 (MYB), SINPZ0401118 (NAC), SINPZ0500871 (C3H) most highly induced TFs Our findings may enhance quality breeding.

Language: Английский

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Production and achievements of Sesamum indicum industry in the world: Past and current state

Oil Crop Science, Journal Year: 2024, Volume and Issue: 9(3), P. 187 - 197

Published: July 1, 2024

Sesame production is important in agriculture, food industry, and the crop diversity due to its rich nutritional profile health benefits. Despite significant value, sesame still an orphan that has received little scientific attention, resulting low yield compared other major oilseed crops. This review offers a comprehensive overview of present state production, knowledge, research advancements concerning Sesamum indicum on global scale. The FAOSTAT database was extensively used examine trends from 1961 2021. In past 60 years, substantially increased, with Asia Africa being primary producers. integration omics technologies biotechnological interventions revolutionized our understanding genetic basis sesame, enhanced productivity, invigorated stress resilience, improved seed quality. High-throughput sequencing methods such as RNA-seq, RAD-seq, SLAF-seq, GBS technology are various studies, linkage mapping, identification trait-associated markers. Fine maps, multi-omics studies genomics, proteomics, transcriptomics, metabolomics have been employed for gene QTL mapping. Proteins metabolic pathways related oil content, yield, tolerance were reported. Genes QTLs components, drought, salt, osmotic discovered. Candidate genes associated capsule shattering recently revealed. For more achievement it enhance efficiency through mechanization, advanced agricultural practices, knowledge dissemination farmers. MAS should be particularly reinforced. promising opportunity farmers, governments, stakeholders sector.

Language: Английский

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Genomic and co-expression network analyses reveal candidate genes for oil accumulation based on an introgression population in Upland cotton (Gossypium hirsutum)

Theoretical and Applied Genetics, Journal Year: 2024, Volume and Issue: 137(1)

Published: Jan. 1, 2024

Language: Английский

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Omics technologies towards sesame improvement: a review

Molecular Biology Reports, Journal Year: 2023, Volume and Issue: 50(8), P. 6885 - 6899

Published: June 16, 2023

Language: Английский

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Genome-wide association scan and candidate gene analysis for seed coat color in sesame (Sesamum indicum L.)

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 28, 2025

Introduction Seed coat color in sesame is a crucial trait for breeding programs as it closely associated with important characteristics such oil content, protein levels, and disease resistance, which directly influence seed quality market value. Methods This study investigates the genetic basis of 200 Sudanese genotypes grown two consecutive years through comprehensive phenotyping, genomic diversity analysis, genome-wide association studies (GWAS), candidate gene discovery. Results discussion Phenotypic analysis across growing seasons revealed high heritability significant correlations among parameters (L*, a*, b*), indicating strong control over color. The identified distinct clusters accessions, rapid linkage disequilibrium decay suggesting level recombination. GWAS SNPs traits, revealing key regions on chromosomes 3, 6, 9, 12, 13. Candidate highlighted several genes, including DOF zinc finger proteins WRKY transcription factors, may play essential roles pigment biosynthesis pathways. These findings provide valuable insights to enhance desirable traits sesame.

Language: Английский

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Genomics, Pan-Genomics, and Super Pan-Genomics of Major Oilseed Crops

Published: Jan. 1, 2025

Language: Английский

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Adaption of Next-Generation Breeding Tools in the Improvement of Minor Oilseed Crops: Status and Prospects

Published: Jan. 1, 2025

Language: Английский

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Genome-wide identification of TPS genes in sesame and analysis of their expression in response to abiotic stresses

Oil Crop Science, Journal Year: 2023, Volume and Issue: 8(2), P. 81 - 88

Published: May 1, 2023

Trehalose and its precursor, trehalose-6-phosphate, play critical roles in plant metabolism response to abiotic stresses. Trehalose-6-phosphate synthase (TPS) is a key enzyme the trehalose synthesis pathway. Hence this study identified TPS genes sesame (SiTPSs) examined their expression patterns under various Totally, ten SiTPSs were comprehensively characterized. found be unevenly distributed on five out of 13 chromosomes predicted localized chloroplasts vacuoles cells. Phylogenetic analysis classified SiTPS proteins into two groups (I II), which was supported by gene structure conserved motif analyses. Analysis cis-acting elements promoter regions revealed that they might primarily involve developmental environmental responses. exhibited different tissues Most group II (SiTPS4 - SiTPS10) strongly induced drought, salt, waterlogging, osmotic stress. Particularly, SiTPS10 most significantly up-regulated stresses, indicating it candidate for improving tolerance multiple Our results provide insight family fundamental resources genomics studies towards dissecting genes’ functions.

Language: Английский

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Unraveling the Amino Acid Synthesis in Maturity Sesame Seeds Based on the Integrative Analysis of Transcriptome and Metabolome

Food Frontiers, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 13, 2024

ABSTRACT Sesame plays a vital role in food industry due to its high oil yield, antioxidant potential, and substantial protein content. Notably, there are significant differences amino acid composition sesame seeds at various developmental stages. However, the molecular basis regulatory mechanism underlying production largely remain unexplored. To unravel these mechanisms, we analyzed metabolome transcriptome profiles of variety across four distinct growth stages (S1–S4). Our analysis identified total 17 acids, with glutamic (Glu), arginine (Arg), proline (Pro), tyrosine (Tyr) exhibiting significantly higher abundances mature This increased abundance correlated elevated expression genes involved synthesis genes. Using weighted gene co‐expression network analysis, discovered modules associated glutathione metabolism, biosynthesis, proline, synthesis, along candidate that regulate metabolism. differential within pathways resulted variations contents Glu, Arg, Pro, Tyr stage (28 days after flowering, S4) compared other Correlation revealed strong association among enzymes glutamine synthetase (GS), glutamate/aspartate–prephenate aminotransferase (PAT), polyamine oxidase (PAO) suggesting their potential synthesis. findings provide novel insights into accumulation acids during seeds, highlighting key metabolic this process. study lays groundwork for future studies aiming enhance nutritional quality yield varieties.

Language: Английский

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