Unlocking the Power of Human Ferritin: Enhanced Drug Delivery of Aurothiomalate in A2780 Ovarian Cancer Cells

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Aurothiomalate (AuTM) is an FDA-approved antiarthritic gold drug with unique anticancer properties. To enhance its activity, we prepared a bioconjugate human apoferritin (HuHf) by attaching some AuTM moieties to surface protein residues. The reaction of excess yielded single adduct, that was characterized ESI MS and ICP-OES analysis, using three mutant ferritins trypsinization experiments. adduct contains ~3 atoms per ferritin subunit, arranged in small cluster bound Cys90 Cys102. MD simulations provided plausible structural model for the cluster. evaluated pharmacological properties found be significantly more cytotoxic than free against A2780 cancer cells mainly due higher uptake. NMR-metabolomics showed HuHf induced qualitatively similar changes treated cells, indicating effects on cell metabolism are approximately same, agreement independent biochemical In conclusion, have demonstrated here molecularly precise formed between exhibits far superior drug, while retaining key mechanistic features. Evidence can serve as excellent carrier this metallodrug.

Language: Английский

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Trends, key contributors, and emerging issues in honey and breast cancer: A bibliometric analysis from 2014 to 2024

F1000Research, Journal Year: 2025, Volume and Issue: 14, P. 17 - 17

Published: March 24, 2025

Background Honey, a natural product with diverse bioactive compounds, has been increasingly explored for its potential anticancer properties. This study aims to comprehensively analyze the scientific literature on relationship between honey and breast cancer. Methods A bibliometric analysis was conducted using ScienceDirect database identify publications from 2014 2024. Data publication trends, author collaboration, keyword were extracted gain insight into research landscape. Keyword identified nine distinct clusters, indicating directions regarding role of in cancer treatment. Results In Key journals such as Journal Ethnopharmacology Food Chemistry have at forefront disseminating findings this domain, demonstrating strongly interdisciplinary approach that bridges traditional medicine modern inquiry. The increasing interest properties honey, evidenced by growing number studies, underlines promising agent prevention Conclusions provides comprehensive overview current knowledge landscape highlights emerging issues require further exploration.

Language: Английский

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A metabolic profiling approach to characterize and discriminate plant-based beverages and milk

Journal of Dairy Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

The rising demand for nondairy and nonanimal protein sources has increased plant-based beverages (PBB) consumption. However, research on their functional properties, metabolic profile, discrimination potential is limited. This study evaluated the of proton nuclear magnetic resonance (1H NMR) spectroscopy as an authentication method to discriminate milk (cow goat) PBB macro-groups, including soy-based, fruit-based (almond coconut), cereal-based (rice oat) beverages, based profile. A total 22 (soy-, almond-, coconut-, rice-, oat-based beverages), 4 cow milk, goat cartons were analyzed with 1H NMR obtain Relevant metabolites macro-groups identified through Mann-Whitney U test partial least squares-discriminant analysis. Results revealed that uridine diphosphate, glucose, adenosine key identification milk. At same time, choline guanosine emerged important markers different macro-group detection. In addition, lactose played a significant role in differentiating from PBB. conclusion, these findings represent initial step toward applying nutritional analysis PBB, opening door further into authenticity profiling.

Language: Английский

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Multi-pathway blood biomarkers to target and monitor multidimensional prevention of cognitive and functional decline (nested in the IN-TeMPO study framed within the world-wide FINGERS network)

Frontiers in Aging Neuroscience, Journal Year: 2025, Volume and Issue: 17

Published: May 7, 2025

Background As the population ages, identification of preventive strategies able to delay cognitive and functional decline associated with aging represents a major challenge. To date, multidimensional approaches seem be effective in reducing or delaying onset age-related diseases. Objectives The multicentric randomized controlled trial IN-TeMPO (ItaliaN study Tailored Multidomain interventions Prevent community-dwelling Older adults, ClinicalTrials.gov ID NCT06248723), framed within World-Wide FINGERS network, aims verify efficacy guided multidomain preventing decline. Within this study, we will explore comprehensive array established exploratory blood biomarkers several pathologic processes, including Alzheimer’s disease (AD), neurodegeneration, inflammation, senescence sarcopenia, stratify subject risk assess effect on biomarkers. Design participants ApoE4 status plasma p-tau217 NfL (neurodegeneration), GFAP IL-6 (inflammation), GDF-15 (senescence/sarcopenia) evaluated all subjects ( n = 1,662) both at baseline end (12 months). Exploratory additional measured same time points subgroup 100 subjects: BDNF, ghrelin, IGF-1, irisin redox as markers sarcopenia/senescence oxidative stress, gamma-H2AX PBMCs marker senescence, amyloid beta aggregates plasma, urine erythrocytes supportive AD. Untargeted metabolomics analysis untargeted volatilomics whole performed molecular alterations that may pathogenesis progression diseases frail older adults aim identifying novel potential Conclusion clinical use multiple laboratory can contribute early trajectories mechanisms underlying multidisciplinary pathological processes.

Language: Английский

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NMR-based metabolomics in Alzheimer’s disease research: a review

Frontiers in Molecular Biosciences, Journal Year: 2023, Volume and Issue: 10

Published: Nov. 30, 2023

Alzheimer's disease (AD) is a progressive neurodegenerative disorder and represents the most common cause of dementia in elderly population worldwide. Currently, there no cure for AD, continuous increase number susceptible individuals poses one significant emerging threats to public health. However, molecular pathways involved onset progression AD are not fully understood. This information crucial developing less invasive diagnostic instruments discovering novel potential therapeutic targets. Metabolomics studies complete ensemble endogenous exogenous metabolites present biological specimens may provide an interesting approach identify alterations multiple biochemical processes associated with evolution. In this mini review, we summarize results from metabolomic conducted using nuclear magnetic resonance (NMR) spectroscopy on human samples (blood derivatives, cerebrospinal fluid, urine, saliva, tissues) patients. We describe metabolic identified patients compared controls diagnosed mild cognitive impairment (MCI). Moreover, discuss challenges issues application NMR-based metabolomics context research.

Language: Английский

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Cell Metabolomics to Guide the Design of Metal-Based Compounds

Inorganics, Journal Year: 2024, Volume and Issue: 12(6), P. 168 - 168

Published: June 15, 2024

Despite the increasing interest in development of novel metal-based compounds for cancer treatment, these molecules are currently poorly characterized mechanistic terms, due to their multiple macromolecular targets inside cells. In this review, we show how 1H NMR metabolomics provides a powerful tool investigate metabolic perturbations induced by metal-compounds The chemical identity and concentration metabolites detected cell lysates respective growth media can be viewed as global fingerprint that describes response drug treatment. framework, applications NMR-based study cellular effects treatment cells with anticancer comprehensively reviewed.

Language: Английский

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NMR Metabolomics of Primary Ovarian Cancer Cells in Comparison to Established Cisplatin-Resistant and -Sensitive Cell Lines

Cells, Journal Year: 2024, Volume and Issue: 13(8), P. 661 - 661

Published: April 9, 2024

Cancer cell lines are frequently used in metabolomics, such as vitro tumor models. In particular, A2780 cells commonly a model for ovarian cancer to evaluate the effects of drug treatment. Here, we compare NMR metabolomics profiles and cisplatin-resistant with those derived from 10 patients high-grade serous carcinoma (collected during primary cytoreduction before any chemotherapeutic treatment). Our analysis reveals substantial similarity among all but significant differences between them both cells. Notably, patient-derived closer resistant when considering exo-metabolome, whereas they essentially equidistant A2780-resistant terms endo-metabolome. This behavior results dissimilarities levels several metabolites attributable differential modulation underlying biochemical pathways. The most pronounced glycolytic phenotype, mainly diverge others due alterations few specific already known markers resistance.

Language: Английский

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Integrated Metabolomics and Proteomics of Symptomatic and Early Presymptomatic States of Colitis

Journal of Proteome Research, Journal Year: 2024, Volume and Issue: 23(4), P. 1420 - 1432

Published: March 18, 2024

Colitis has a multifactorial pathogenesis with strong cross-talk among microbiota, hypoxia, and tissue metabolism. Here, we aimed to characterize the molecular signature of disease in symptomatic presymptomatic stages inflammatory process at fecal level. The study is based on two different murine models for colitis, HR-MAS NMR "intact" colon tissues LC-MS/MS extracts were used derive untargeted metabolomics proteomics information, respectively. Solution was metabolomic profiles extracts. By combining proteomic analyses tissues, found increased anaerobic glycolysis, accompanied by an altered citric acid cycle oxidative phosphorylation inflamed colons; these changes associate inflammation-induced hypoxia taking place tissues. Different colitis states also characterized significantly extracts, attributable both dysbiosis characteristic as well dysregulated Strong distinctive signatures can be detected before onset symptoms. Therefore, provides comprehensive picture accompanying already preclinical stages, highlighting diagnostic potential global diseases.

Language: Английский

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COLMAR1d: A Web Server for Automated, Quantitative One-Dimensional Nuclear Magnetic Resonance-Based Metabolomics at Arbitrary Magnetic Fields

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 20, 2024

The field of metabolomics, which is quintessential in today's omics research, involves the large-scale detection, identification, and quantification small-molecule metabolites a wide range biological samples. Nuclear magnetic resonance spectroscopy (NMR) has emerged as powerful tool for metabolomics due to its high resolution, reproducibility, exceptional quantitative nature. One key bottlenecks studies, however, remains accurate automated analysis resulting NMR spectra with good accuracy minimal human intervention. Here, we present COLMAR1d platform, consisting public web server an optimized database, one-dimensional (1D) NMR-based address these challenges. database comprises more than 480 from GISSMO enabling query measured at arbitrary strengths, demonstrated acquired between

Language: Английский

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Unlocking the Power of Human Ferritin: Enhanced Drug Delivery of Aurothiomalate in A2780 Ovarian Cancer Cells

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(40)

Published: July 1, 2024

Abstract Aurothiomalate (AuTM) is an FDA‐approved antiarthritic gold drug with unique anticancer properties. To enhance its activity, we prepared a bioconjugate human apoferritin (HuHf) by attaching some AuTM moieties to surface protein residues. The reaction of excess yielded single adduct, that was characterized ESI MS and ICP‐OES analysis, using three mutant ferritins trypsinization experiments. adduct contains ~3 atoms per ferritin subunit, arranged in small cluster bound Cys90 Cys102. MD simulations provided plausible structural model for the cluster. evaluated pharmacological properties found be significantly more cytotoxic than free against A2780 cancer cells mainly due higher uptake. NMR‐metabolomics showed HuHf induced qualitatively similar changes treated cells, indicating effects on cell metabolism are approximately same, agreement independent biochemical In conclusion, have demonstrated here molecularly precise formed between exhibits far superior drug, while retaining key mechanistic features. Evidence can serve as excellent carrier this metallodrug.

Language: Английский

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