Tangeretin Improves the Memory of Swiss Mice, Suggesting Potential Molecular Interventions Through Animal Behavior Assessments and In Silico Studies DOI Creative Commons
Md. Sakib Al Hasan, Mohd Shahnawaz Khan, Ayub Ali

et al.

Brain and Behavior, Journal Year: 2025, Volume and Issue: 15(5)

Published: May 1, 2025

ABSTRACT Introduction Tangeretin (TAN), a polymethoxylated flavone from citrus peels, exhibits neuroprotective, anti‐inflammatory, and antioxidant properties. This study aims to evaluate the memory‐enhancing effects of TAN in Swiss mice explore its potential molecular interactions with D 2 dopamine (DOP) receptor through vivo behavioral assessments silico approaches. Methods were administered (10 20 mg/kg), DOP (22 olanzapine (OLN) (2 alone combinations per orally (p.o.), followed by cognitive using marble burying, dust removal, trained swimming tests. In studies included docking against (PDB: 6CM4), pharmacokinetics (SwissADME, pkCSM), toxicity predictions (ProTox‐3). Results significantly ( p < 0.05) improved functions, including memory, anxiety, motor coordination, dose‐dependent manner, mg/kg showing most notable effect. The combination TAN‐10 DOP‐22 enhanced these benefits, whereas OLN‐2 reduced improvements. TAN‐treated showed better performance tests, indicating problem‐solving, coordination. These results suggest TAN's enhancement, particularly DOP‐22. No deaths observed any treatment group, all treated animals exhibited normal physiological activity no signs acute toxicity. revealed that strongest binding affinity (BA) (−6.6 kcal/mol) receptor, forming multiple hydrogen bonds (HBs), which indicates mechanism for memory enhancement via dopaminergic modulation. Pharmacokinetic analyses also has favorable ADMET properties, high gastrointestinal absorption, blood–brain barrier penetration, low Conclusion findings highlight as promising therapeutic candidate memory‐related disorders, warranting further clinical exploration.

Language: Английский

Assessment of Antidiarrheal Effect of Oleuropein Through µ‐Oopioid Receptor Interaction Pathway: In Vivo and in Silico Studies DOI Open Access
Nishat Jahan,

Manoj Mandal,

Imam Hossen Rakib

et al.

Drug Development Research, Journal Year: 2025, Volume and Issue: 86(1)

Published: Feb. 1, 2025

Oleuropein (OLP), a compound predominantly found in olive leaves, has been known for its numerous biological activities, including antioxidant, anti-inflammatory, and antimicrobial properties. Despite established therapeutic potential, role treating diarrhea not extensively explored. This study aimed to evaluate the antidiarrheal effects of OLP an vivo model investigate molecular interactions using silico docking studies, pharmacokinetic predictions, toxicity analysis. In study, castor oil was used induce 3-day-old chicks, effect tested at doses 10 20 mg/kg. The standard drug, loperamide (LOP) 3 mg/kg, comparison. results showed that both significantly (p < 0.05) reduced diarrheal secretions increased latency, with mg/kg dose demonstrating most effective results. combination (20 mg/kg) LOP (3 further enhanced effect. revealed exhibited strong binding affinities (BAs) key receptor, μ-opioid receptor associated diarrhea, while higher BA (‒8.9 kcal/mol) compared (‒8.7 kcal/mol). Pharmacokinetic analysis favorable properties studies no acute toxicity, LD50 2000 conclusion, findings suggest possesses significant potential through interaction, positioning it as promising natural agent managing diarrhea. Further are warranted fully elucidate mechanisms action clinical applicability.

Language: Английский

Citations

1
Tangeretin enhances sedative activity of diazepam in Swiss mice through GABAA receptor interaction: In vivo and in silico approaches DOI
Md. Sakib Al Hasan, Md. Shimul Bhuia, Raihan Chowdhury

et al.

Neuroscience, Journal Year: 2025, Volume and Issue: 572, P. 1 - 10

Published: March 5, 2025

Language: Английский

Citations

1
Assessment of antiemetic and modulatory activity of dihydrocoumarin on copper sulfate induced emetic chicks: An in vivo investigation DOI Creative Commons
Kritika Sharma, Md. Sakib Al Hasan, Razina Rouf

et al.

Food Chemistry Advances, Journal Year: 2025, Volume and Issue: 6, P. 100930 - 100930

Published: Feb. 23, 2025

Language: Английский

Citations

0
Anti-arthritic potential of linalool: in vitro, in vivo, and in silico mechanistic insights for safer therapeutic applications DOI

S U Ahammed,

Raihan Chowdhury, Md. Sakib Al Hasan

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Language: Английский

Citations

0
Caffeine and sclareol take the edge off the sedative effects of linalool, possibly through the GABAA interaction pathway: molecular insights through in vivo and in silico studies DOI
Muhammad Torequl Islam, Md. Sakib Al Hasan, Jannatul Ferdous

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Language: Английский

Citations

0
Citronellal Exerts Sedative‐Like Effects and Augments Diazepam's Action in Swiss Mice, Possibly Through the GABAergic Pathway DOI Creative Commons
Muhammad Torequl Islam, Md. Sakib Al Hasan,

Emon Mia

et al.

Brain and Behavior, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 1, 2025

ABSTRACT Introduction Citronellal (CTL), a monoterpenoid, exhibits notable neurological activity, including anxiolytic, and anticonvulsant effects, primarily through GABAergic pathways. Our current study aimed to explore CTL's sedative potential using in vitro, vivo, silico approaches the pathway. Methods The vitro activity of CTL was assessed via colorimetric assay, while acute toxicity evaluated Swiss mice per OECD guidelines with doses up 2000 mg/kg establish safety margins. Sedative effects were thiopental sodium (TS, 40 mg/kg)‐induced sleep protocols. administered at 62.5, 125, 250 doses, alone or combined diazepam (DZP, 2 mg/kg) flumazenil (FLU, 0.1 mg/kg). studies also performed GABA A receptors (α1 β2 subunits) investigate possible molecular mechanism. Results results demonstrated that exhibited significantly concentration‐dependent activity. Acute tests indicated high margin (no behavioral physiological abnormalities dose). Additionally, ( p < 0.05) dose‐dependently reduced latency augmented duration animals, compared control group. It decreased increased DZP‐2 reducing this parameter FLU‐0.1. In studies, binding affinities (BAs) receptor –5.6 kcal/mol. Conclusion potent strong profile interaction subunits).

Language: Английский

Citations

0
Assessment of Antihyperglycemic and Modulatory Effects of trans‐Ferulic Acid on Glibenclamide and Metformin: In Vivo and Molecular Docking Studies DOI
Md. Sakib Al Hasan, Md. Shimul Bhuia, Salehin Sheikh

et al.

ChemistrySelect, Journal Year: 2025, Volume and Issue: 10(14)

Published: April 1, 2025

Abstract Diabetes mellitus (DM) is a chronic metabolic disorder characterized by persistent hyperglycemia due to impaired insulin secretion, resistance, or both. trans ‐Ferulic acid (TFA) has several biological activities, including sedative, anxiolytic, anti‐inflammatory, and so on. However, there no in vivo silico study on the antihyperglycemic activity of TFA. This assessed antidiabetic potential TFA its modulatory effect glibenclamide (GLI) metformin (MET) through hypoglycemic tests mice molecular docking targeting glucose metabolism proteins. (25, 50, 75 mg/kg, p.o.) was tested glucose‐induced hyperglycemic alongside GLI (10 MET (100 p.o.), with blood levels (BGLs) monitored. significantly ( p < 0.05) reduced BGLs dose‐dependent manner, mg/kg dose achieving 30.99% reduction at 180 min, compared 52.04% 55.27% (MET). Combination therapy (TFA‐50+GLI‐10 TFA‐50+MET‐100) further enhanced glucose‐lowering effects, TFA‐50+GLI‐10 showing highest (58.90%). In analysis revealed that exhibited elevated binding affinities complex I (−6.4 kcal/mol) mitochondrial glycerol‐3‐phosphate dehydrogenase (−6.8 kcal/mol). These findings suggest significant potential, supporting role regulation.

Language: Английский

Citations

0
Anticancer activity of Jasminum sambac and its bioactive phytochemicals against the PI3K-AKT-mTOR pathway: A literature-based in silico study DOI
Md. Sakib Al Hasan, Md. Shimul Bhuia, Raihan Chowdhury

et al.

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 180, P. 431 - 443

Published: March 23, 2025

Language: Английский

Citations

0
Tangeretin Improves the Memory of Swiss Mice, Suggesting Potential Molecular Interventions Through Animal Behavior Assessments and In Silico Studies DOI Creative Commons
Md. Sakib Al Hasan, Mohd Shahnawaz Khan, Ayub Ali

et al.

Brain and Behavior, Journal Year: 2025, Volume and Issue: 15(5)

Published: May 1, 2025

ABSTRACT Introduction Tangeretin (TAN), a polymethoxylated flavone from citrus peels, exhibits neuroprotective, anti‐inflammatory, and antioxidant properties. This study aims to evaluate the memory‐enhancing effects of TAN in Swiss mice explore its potential molecular interactions with D 2 dopamine (DOP) receptor through vivo behavioral assessments silico approaches. Methods were administered (10 20 mg/kg), DOP (22 olanzapine (OLN) (2 alone combinations per orally (p.o.), followed by cognitive using marble burying, dust removal, trained swimming tests. In studies included docking against (PDB: 6CM4), pharmacokinetics (SwissADME, pkCSM), toxicity predictions (ProTox‐3). Results significantly ( p < 0.05) improved functions, including memory, anxiety, motor coordination, dose‐dependent manner, mg/kg showing most notable effect. The combination TAN‐10 DOP‐22 enhanced these benefits, whereas OLN‐2 reduced improvements. TAN‐treated showed better performance tests, indicating problem‐solving, coordination. These results suggest TAN's enhancement, particularly DOP‐22. No deaths observed any treatment group, all treated animals exhibited normal physiological activity no signs acute toxicity. revealed that strongest binding affinity (BA) (−6.6 kcal/mol) receptor, forming multiple hydrogen bonds (HBs), which indicates mechanism for memory enhancement via dopaminergic modulation. Pharmacokinetic analyses also has favorable ADMET properties, high gastrointestinal absorption, blood–brain barrier penetration, low Conclusion findings highlight as promising therapeutic candidate memory‐related disorders, warranting further clinical exploration.

Language: Английский

Citations

0