The construction of a peptide vaccine with multifunctional DNA tetrahedron exoskeleton DOI

Jiale Liang,

X. S. Qin, Siqi Xu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 157911 - 157911

Published: Nov. 1, 2024

Language: Английский

Recent progress of non-linear topological structure polymers: synthesis, and gene delivery DOI Creative Commons
Chenfei Wang, Wei He,

Feifei Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 27, 2024

Abstract Currently, many types of non-linear topological structure polymers, such as brush-shaped, star, branched and dendritic structures, have captured much attention in the field gene delivery nanomedicine. Compared with linear structural polymers offer advantages, including multiple terminal groups, broad complicated spatial architecture multi-functionality sites to enhance efficiency targeting capabilities. Nevertheless, complexity their synthesis process severely hampers development applications nonlinear polymers. This review aims highlight various synthetic approaches reversible-deactivation radical polymerization (RDRP) atom-transfer (ATRP), nitroxide-mediated (NMP), reversible addition-fragmentation chain transfer (RAFT) polymerization, click chemistry reactions Michael addition, thoroughly discuss advantages disadvantages, well analyze further application potential. Finally, we comprehensively summarize different for genetic materials delivering performance both vitro vivo, which indicated that effects topologies play a crucial role enhancing transfection polymeric vectors. offered promising guideline design novel facilitated new generation polymer-based

Language: Английский

Citations

8
Polymer-mediated protein/peptide therapeutic stabilization: Current progress and future directions DOI

P S Rajalakshmi,

Katherine Snell,

Grace E. Kunkel

et al.

Progress in Polymer Science, Journal Year: 2024, Volume and Issue: 156, P. 101867 - 101867

Published: Aug. 8, 2024

Language: Английский

Citations

7
Nanomaterials Enhance Pyroptosis-Based Tumor Immunotherapy DOI Creative Commons
Fujian Ji,

Chunyu Shi,

Zhenbo Shu

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 5545 - 5579

Published: June 1, 2024

Pyroptosis, a pro-inflammatory and lytic programmed cell death pathway, possesses great potential for antitumor immunotherapy. By releasing cellular contents large number of factors, tumor pyroptosis can promote dendritic maturation, increase the intratumoral infiltration cytotoxic T cells natural killer cells, reduce immunosuppressive within tumor. However, efficient induction prevention damage to normal tissues or is an urgent concern be addressed. Recently, wide variety nanoplatforms have been designed precisely trigger activate immune responses. This review provides update on progress in nanotechnology enhancing pyroptosis-based Nanomaterials shown advantages triggering by delivering initiators tumors, increasing oxidative stress inducing intracellular osmotic pressure changes ion imbalances. In addition, challenges future perspectives this field are proposed advance clinical translation pyroptosis-inducing nanomedicines.

Language: Английский

Citations

4
Strategies and Challenges of Cytosolic Delivery of Proteins DOI

Yuanyuan Li,

Baojie Du,

Lichao Yu

et al.

Journal of drug targeting, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 32

Published: Jan. 25, 2025

The cytosolic delivery of therapeutic proteins represents a promising strategy for addressing diseases caused by protein dysfunction. Despite significant advances, efficient remains challenging due to barriers such as cell membrane impermeability, endosomal sequestration, and instability. This review summarizes recent progress in systems, including physical, chemical, biological approaches, with particular focus on strategies that enhance escape targeting specificity. We further discuss the clinical translatability these approaches propose future directions improving efficiency safety, ultimately unlocking potential intracellular proteins.

Language: Английский

Citations

0
Needle-free injection devices for targeted oral delivery of macromolecules DOI

Zhonglin Fang,

Zhuohao Zhang, Luoran Shang

et al.

Science Bulletin, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Self-assembling chemically modified siRNA nanorings for RNAi therapy and neuroprotection in Alzheimer’s disease DOI
Yifan Jiang, Lisha Li, Xiao Fang

et al.

Science China Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Language: Английский

Citations

0
Synthetic Polymers for Drug, Gene, and Vaccine Delivery DOI Creative Commons
Yajun Xu, Jie Chen, Jianxun Ding

et al.

Polymer science & technology., Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Language: Английский

Citations

0
Sequentially assembled co-delivery nanoplatform of SIRT1 protein and SOX9-expressing plasmid for multipronged therapy of intervertebral disc degeneration DOI Creative Commons
Xiaoyu Zhang, Qianping Guo,

J. Fang

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: May 10, 2025

Nucleus pulposus cells (NPCs) undergo metabolic disorders and matrix pathological remodeling under the influence of various adverse factors during intervertebral disc degeneration (IVDD), whereas post-translational modifications (PTMs) can confer with capacity to respond quickly adapt complex environmental changes. Here, SIRT1 protein, a key regulator within PTMs framework, was applied against hostile degenerative microenvironment. Then, it sequentially assembled SOX9-expressing plasmid, an essential transcription factor promote extracellular (ECM) biosynthesis, onto phenylboronic acid-functionalized G5-dendrimer construct multifunctional nanoplatform for IVDD therapy. In vitro, nanoplatforms showed antioxidant capacity, ability restore mitochondrial homeostasis normal ECM metabolism, as well maintain cellular phenotypes. RNA sequencing suggested that inhibition Nod-like receptor signaling might be mechanism behind their therapeutic effects. The were then wrapped in designed dynamic hydrogel, not only prolonging retention time loaded cargoes, but also maintaining structure, height, water content vivo. Overall, this study presents convenient strategy inhibit multiple factors, hold promise treatment.

Language: Английский

Citations

0
Synthesis and intracellular basic protein delivery of a polyanionic flexible organic framework DOI Open Access

Yue-Yang Liu,

Yan Wu, Peng Guo

et al.

Chemical Synthesis, Journal Year: 2024, Volume and Issue: 4(2)

Published: June 26, 2024

Although cationic porous polymers have been widely used for gene and drug delivery, the delivering function of anionic has rarely explored. Herein, we prepare a polyanionic flexible organic framework (pa-FOF) through quantitative formation acylhydrazone bond from tetraanionic tetraaldehyde diacylhydrazine. Pa-FOF is highly water-soluble size 26 to 51 nm, which depends on concentration monomers, an aperture approximately 3.8 nm. Fluorescence, zeta potential, confocal laser scanning microscopic flow cytometric experiments reveal that pa-FOF can adsorb basic proteins, including lysozyme, trypsin cytochrome c, driven by intermolecular ion-pairing electrostatic attraction hydrophobicity, realizes efficient intracellular delivery adsorbed proteins. Confocal imaging further illustrate c significantly increase its ability causing cell apoptosis.

Language: Английский

Citations

2
Mechanism study of cross presentation of exogenous antigen induced by cholera toxin-like chimeric protein DOI

Xianying He,

Kaixiang Fan,

Haiyan Gong

et al.

Vaccine, Journal Year: 2024, Volume and Issue: 42(7), P. 1549 - 1560

Published: Feb. 5, 2024

Language: Английский

Citations

1