Heat stable and intrinsically sterile liquid protein formulations DOI Creative Commons
Atip Lawanprasert, Harminder Singh,

Sopida Pimcharoen

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 30, 2024

Over 80% of biologic drugs, and 90% vaccines, require temperature-controlled conditions throughout the supply chain to minimize thermal inactivation contamination. This cold is costly, requires stringent oversight, impractical in remote environments. Here, we report chemical dispersants that non-covalently solvate proteins within fluorous liquids alter their thermodynamic equilibrium reduce conformational flexibility. generates non-aqueous, fluorine-based liquid protein formulations biochemically rigidify structure yield thermally stable biologics at extreme temperatures (up 90 °C). These non-aqueous are impervious contamination by microorganismal pathogens, degradative enzymes, environmental impurities, display comparable pre-clinical pharmacokinetics safety profiles standard saline samples. As a result, deliver fluorochemical formulation paradigm may limit need for logistics reagents biopharmaceuticals.

Language: Английский

Self-Assembled Protein–Polymer Nanoparticles via Photoinitiated Polymerization-Induced Self-Assembly for Targeted and Enhanced Drug Delivery in Cancer Therapy DOI Creative Commons
Gayathri R. Ediriweera, Yi-Xin Chang, Wenting Yang

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 856 - 856

Published: Feb. 13, 2025

Protein-polymer bioconjugates offer numerous advantages in biomedical applications by integrating the benefits of functional proteins and tunable synthetic polymers. Developing drug-loaded protein-polymer nanoparticles, with a receptor-targeting protein forming nanoparticle shell, would be ideal for targeted delivery drugs to cancer cells that overexpress specific receptors more effective therapy. In this study, we report synthesis reduction-responsive nanoparticles photoinitiated polymerization-induced self-assembly (photo-PISA) approach. Anti-cancer can efficiently encapsulated at high concentrations within during photo-PISA process. These present transferrin (Tf) on their surfaces, capable targeting overexpressed Tf found cells. It was demonstrate enhanced cellular uptake anti-cancer drug, curcumin, via receptor-mediated endocytosis, compared control PEGylated lack capability. Moreover, release curcumin response reducing environment, characteristic health Consequently, synthesized are inducing cell death demonstrating potential as an drug system

Language: Английский

Citations

2
Disordered peptide-based design of intrinsically disordered polymers for biomedical applications DOI
Orkid Coskuner‐Weber,

Fatma Nilsu Cayli,

Vladimir N. Uversky

et al.

International Journal of Polymer Analysis and Characterization, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 49

Published: Feb. 10, 2025

Language: Английский

Citations

0
Autonomous Abiotic Thermal Protectant for Immunoglobulin G: Reducing the Need for Cold Chain Storage DOI Creative Commons

Beverly Chou,

Rishad J. Dalal,

Kenneth J. Shea

et al.

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Antibodies are vital biologic therapeutics, but their impact is limited by thermal instability. This requires maintaining a cold chain, from the point of manufacture to use. We report an approach that could reduce need for chain. present protectant (TP) immunoglobulin G (IgG) mimics behavior heat shock protein HSP60. hydrogel copolymer nanoparticle shows minimal affinity IgG at or below 25 °C. As temperatures rise and proteins melting temperature (Tm), TP undergoes autonomous phase transition (∼27 °C), above which high sequestering stabilizing far Tm. return RT, reverts its water-swollen state, allowing any metastable time refold native state before being released. The optimized has very low molar capacity, effectively isolating preventing aggregation elevated temperatures.

Language: Английский

Citations

0
Site-Selective Zwitterionic Poly(caprolactone-carboxybetaine)-Growth Hormone Receptor Antagonist Conjugate: Synthesis and Biological Evaluation DOI

Jane K. Yang,

Madeline B. Gelb, Kyle Tamshen

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Zwitterionic polymers have been found to be biocompatible alternatives poly(ethylene glycol) (PEG) for conjugation proteins. This work reports the site-selective of poly(caprolactone-carboxybetaine) (pCLZ) human growth hormone receptor antagonist (GHA) B2036-alkyne and investigation safety, activity, pharmacokinetics. Azide-end-functionalized pCLZs were synthesized conjugated GHA via copper-catalyzed click reaction. The resulting inhibitory bioactivity concentration responses in Ba/F3-GHR cells compared those PEGylated B2036. IgG IgM antibody production was tested mice, no measurable or cytokine detected pCLZ conjugate. Using

Language: Английский

Citations

1
Oxygen-tolerant photo-RAFT enables in-situ synthesis of protein-based nanoparticles DOI Creative Commons
Vinod K. Kannaujiya, Tong Zhang, Md Aquib

et al.

European Polymer Journal, Journal Year: 2024, Volume and Issue: unknown, P. 113518 - 113518

Published: Oct. 1, 2024

Language: Английский

Citations

0
Heat stable and intrinsically sterile liquid protein formulations DOI Creative Commons
Atip Lawanprasert, Harminder Singh,

Sopida Pimcharoen

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 30, 2024

Over 80% of biologic drugs, and 90% vaccines, require temperature-controlled conditions throughout the supply chain to minimize thermal inactivation contamination. This cold is costly, requires stringent oversight, impractical in remote environments. Here, we report chemical dispersants that non-covalently solvate proteins within fluorous liquids alter their thermodynamic equilibrium reduce conformational flexibility. generates non-aqueous, fluorine-based liquid protein formulations biochemically rigidify structure yield thermally stable biologics at extreme temperatures (up 90 °C). These non-aqueous are impervious contamination by microorganismal pathogens, degradative enzymes, environmental impurities, display comparable pre-clinical pharmacokinetics safety profiles standard saline samples. As a result, deliver fluorochemical formulation paradigm may limit need for logistics reagents biopharmaceuticals.

Language: Английский

Citations

0