Curcumin and Methotrexate: A Promising Combination for Osteosarcoma Treatment via Hedgehog Pathway Inhibition DOI Open Access

Giulia Giliberti,

Maria Maddalena Marrapodi,

Giuseppe Di Feo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11300 - 11300

Published: Oct. 21, 2024

Osteosarcoma (OS) is the most severe bone tumor in children. A chemotherapy regimen includes a combination of high-dose Methotrexate (MTX), doxorubicin, and cisplatin. These drugs cause acute chronic side effects, such as infections, thrombocytopenia, neutropenia, DNA damage, inflammation. Therefore, to identify new therapeutic strategies, effective with safety profile, necessary. The Hedgehog (Hh) signaling pathway involved tumorigenesis active OS. Hh components Patched receptor 1 (PTCH1), Smoothened (SMO), glioma-associated oncogene homolog transcription factors (GLI1 GLI2) are overexpressed OS cell lines patient samples. Curcumin (CUR)-with antioxidant anti-cancer properties-downregulates cancer, inhibiting progression. This study investigates CUR effects on MG-63 line, alone combined MTX, propose novel approach. Our suggests agent OS, particularly when MTX. Targeting pathway, MTX showed significant pro-apoptotic increasing BAX/Bcl-2 ratio total apoptotic percentage. They reduced expression (PTCH1, SMO, GLI1, GLI2), proliferation, survival, invasion. determined β-Catenin decrease trend toward reducing NF-kB matrix metalloproteinases (MMP-2 MMP-9). findings suggest support treatment, improving outcomes adverse current therapies.

Language: Английский

Wnt/β-catenin signaling pathway: an attractive potential therapeutic target in osteosarcoma DOI Creative Commons
Yi Ding, Qin Chen

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 14

Published: Feb. 14, 2025

Osteosarcoma (OS) is the most common bone malignancy in children and adolescents, although current neoadjuvant chemotherapy has shown efficacy against OS, long-term survival rate for patients with OS remains low, highlighting need to find more effective treatments. In cancer cells, abnormal activation of signaling pathways can widely affect cell activity from growth proliferation apoptosis, invasion metastasis. Wnt/β-catenin a complex unique pathway that considered be one important carcinogenic human cancer. Research have confirmed an driving factor occurrence development osteosarcoma, this promote pathological processes proliferation, invasion, migration, tumor angiogenesis chemical resistance osteosarcoma. However, inhibition effectively inhibit or reverse above processes. Therefore, manipulating expression function may potential targeted treatment OS. review, we describe characteristics summarize role mechanism This paper discusses therapeutic significance inhibiting targeting shortcomings studies on problems solved. review helps us understand provides theoretical basis new ideas as target

Language: Английский

Citations

0
The role of lncRNA and miRNA on the effects of occurrence and development of osteosarcoma DOI
Xiaobo Zhang,

Xidan Gao,

Jing Xu

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 144, P. 113726 - 113726

Published: Nov. 29, 2024

Language: Английский

Citations

2
Bibliometric analysis and visualisation of research hotspots and frontiers on omics in osteosarcoma DOI Creative Commons
Xinyu Wang,

Xin Cao,

Zhongshang Dai

et al.

Journal of Cancer Research and Clinical Oncology, Journal Year: 2024, Volume and Issue: 150(8)

Published: Aug. 22, 2024

Omics technology has become a widely applied biological science that can be used to study the etiology, pathogenesis, and treatment of osteosarcoma(OS). Bibliometric analysis is still blank in this field.This aimed access trends hotspots omics OS research through bibliometric method. Relevant articles reviews from 1999 2023 were retrieved Web Science Core Collection. The data processed with CiteSpace, some graphs generated Graphpad, VOSviewer, Scimago Graphica, Bibliometrix R Studio. A total 1581 papers included. China (569, 36.0%) United States (523, 33.1%) took dominant position number published papers, links between countries most frequently occurred North America East Asia, Australia Europe. Top institutions highest publications almost located States, University Texas MD Anderson Cancer Center contributing (44, 2.78%). Among researchers field, Cleton-Jansen AM was author field (20, 1.27%). According keyword cluster analysis, studies focused on "comparative genomic hybridization" before 2012. latest surge words "tumor microenvironment" "immune infiltration" heatmap indicate future directions. Our provided current status global level hottest developing rapidly, main focuses revealing characteristics tumor microenvironment how activate immune system fight cancer cells. Research its relationship genetic aberrations will priority future. osteosarcoma been exploding since 1998 due development emphasis researchers. had shifted discovering new molecular target present trend, mechanisms

Language: Английский

Citations

0
Curcumin and Methotrexate: A Promising Combination for Osteosarcoma Treatment via Hedgehog Pathway Inhibition DOI Open Access

Giulia Giliberti,

Maria Maddalena Marrapodi,

Giuseppe Di Feo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11300 - 11300

Published: Oct. 21, 2024

Osteosarcoma (OS) is the most severe bone tumor in children. A chemotherapy regimen includes a combination of high-dose Methotrexate (MTX), doxorubicin, and cisplatin. These drugs cause acute chronic side effects, such as infections, thrombocytopenia, neutropenia, DNA damage, inflammation. Therefore, to identify new therapeutic strategies, effective with safety profile, necessary. The Hedgehog (Hh) signaling pathway involved tumorigenesis active OS. Hh components Patched receptor 1 (PTCH1), Smoothened (SMO), glioma-associated oncogene homolog transcription factors (GLI1 GLI2) are overexpressed OS cell lines patient samples. Curcumin (CUR)-with antioxidant anti-cancer properties-downregulates cancer, inhibiting progression. This study investigates CUR effects on MG-63 line, alone combined MTX, propose novel approach. Our suggests agent OS, particularly when MTX. Targeting pathway, MTX showed significant pro-apoptotic increasing BAX/Bcl-2 ratio total apoptotic percentage. They reduced expression (PTCH1, SMO, GLI1, GLI2), proliferation, survival, invasion. determined β-Catenin decrease trend toward reducing NF-kB matrix metalloproteinases (MMP-2 MMP-9). findings suggest support treatment, improving outcomes adverse current therapies.

Language: Английский

Citations

0