UJI KATALITIK ENZIM KERATINASE, LIPASE DAN SELULASE ISOLAT ACTINOMYECTES SD-5 DARI PERAIRAN PANTAI MANGROVE OESAPA, KUPANG YANG BERPOTENSI DIKEMBANGKAN SEBAGAI IMBUHAN PAKAN MULTIENZIM DOI Open Access

Priska Bhene,

Stormy Vertygo,

Cytske Sabuna

et al.

PARTNER, Journal Year: 2024, Volume and Issue: 29(2), P. 238 - 238

Published: Nov. 30, 2024

ABSTRAK Pakan berkualitas memegang peranan vital dalam pemeliharaan ternak. Salah satu pendekatan yang dapat digunakan untuk mencapai tuuan ini adalah dengan menambahkan imbuhan pakan ( feed additive ) misalnya berupa enzim. Penelitian bertujuan mengevaluasi bagaimana kemampuan katalitik Enzim Keratinase, Lipase dan Selulase dari isolat Actinomycetes SD-5 berpotensi dikembangkan sebagai ternak multienzim. Isolat telah diisolasi sebelumnya sedimen di kawasan pantai Mangrove Oesapa Barat, Kota Kupang. Uji dilakukan terhadap enzim Keratin, secara berturut-turut menggunakan media Keratin Agar, Lipid Agar Carboxymethyl Cellulose (CMC) Agar. Parameter diamati terbentuknya zona hidrolitik sekitar koloni tumbuh pada meedia uji. Hasil penelitian menunjukkan bahwa mampu menghasilkan ketiga tersebut ditandai zona-zona terbentuk agar Dengan demikian, disimpulkan lebih lanjut multienzim . Kata kunci : Actinomycetes, Lipase, Mangrove, Sedimen, Selulase. ABSTRA CT Quality plays a role in livestock maintenance. One approach to achieve this is by adding additives, such as enzymes. This study aims evaluate the catalytic capabilities of and Cellulase enzymes from isolate SD-5, which has potential be developed multi-enzyme for livestock. was previously obtained sediment mangrove coastal area Kupang City. Catalytic tests were conducted on using media, respectively. The observed parameter formation hydrolytic zones around colonies grown test media. results showed that capable producing all three enzymes, indicated formed Thus, it can concluded further additive. Keywords Sediment, Cellulase.

Tannic acid extracted from gallnut improves intestinal health with regulation of redox homeostasis and gut microbiota of weaned piglets DOI Creative Commons

Zhang‐Chao Deng,

Jie Wang, Juan Wang

et al.

Animal Research and One Health, Journal Year: 2024, Volume and Issue: 2(1), P. 16 - 27

Published: Feb. 1, 2024

Abstract The objective of this study is to evaluate the effects tannic acid (TA) derived from gallnut supplementation on growth performance and health status weaned piglets. A total 432 weanling piglets (7.05 ± 1.05 kg) were randomly allocated into 4 treatment groups with 6 replicates 18 pigs/pen. Piglets fed either a basal diet (CON), or diets supplemented 1.5 kg/t TA, 3.0 1.8 zinc oxide (ZnO) for 21 days. results showed that, compared CON, dietary TA did not affect ( p > 0.05) serum biochemistry However, had higher SOD, GPX, CAT activities lower MDA concentration in jejunum than those CON ZnO group. Meanwhile, increased < villus height height/crypt depth, decreased crypt depth small intestine. Dietary also downregulated IL‐1β TNF‐α expression jejunum. Furthermore, reduced abundance Candidatus Brocadia Escherichia‐Shigella cecal digesta. Notably, both negative correlation antioxidant enzymes R −0.60, 0.01), but was positively correlated concentrations = 0.44, In conclusion, improved gut piglets, potentially by regulating redox homeostasis microbiota.

Language: Английский

Citations

17
Rational engineering S1' substrate binding pocket to enhance substrate specificity and catalytic activity of thermal-stable keratinase for efficient keratin degradation DOI
Xiao‐Dong Pei,

Heliang Fan,

Dao-Quan Jiao

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 263, P. 130688 - 130688

Published: March 6, 2024

Language: Английский

Citations

6
Poultry Nutrition: Achievement, Challenge and Strategy DOI
Kexin Cao,

Zhang‐Chao Deng,

Shijun Li

et al.

Journal of Nutrition, Journal Year: 2024, Volume and Issue: 154(12), P. 3554 - 3565

Published: Oct. 16, 2024

Language: Английский

Citations

5
Evaluation of the effects of Keratinase-treated feather meal as a replacement for fishmeal on growth performance, liver function, and intestinal health in juvenile largemouth bass (Micropterus salmoides) DOI

Yi‐Huan Xu,

Li-Han Zhang, Min Liu

et al.

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 40, P. 102636 - 102636

Published: Jan. 21, 2025

Language: Английский

Citations

0
Keratinous bioresources: their generation, microbial degradation, and value enhancement for biotechnological applications DOI

Vijan Lal Vikash,

Numbi Ramudu Kamini, Ganesan Ponesakki

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(4)

Published: March 29, 2025

Language: Английский

Citations

0
Effect of Partial Substitution of Feather Meal Instead of Soybean Meal on Biochemical and Immunological Characteristics of Ross308 Broiler DOI Open Access
Muhammad Shakir,

Ibrahim Fadhil Al-Zamili,

Jassim Kassim Al-Gharawi

et al.

IOP Conference Series Earth and Environmental Science, Journal Year: 2025, Volume and Issue: 1487(1), P. 012174 - 012174

Published: April 1, 2025

Abstract The current study was conducted at the poultry field, Agriculture College, Al-Muthanna University, from 21/03/2024 to 24/04/2024. 317 broiler chicks of Ross 308 breed, one day old, unsexed and with an initial weight 40±1.0 gm, were used. They distributed randomly into 7 treatments 3 replicates for each treatment (15 replicate). as follows: T1: control treatment. As rest treatments, soybeans replaced feather powder a rate 2% following treatments; T2: partial replacement decomposed by enzyme Keratinase concentration 100,000 IU. T3: 200,000 T4: treated sodium hydroxide NaOH 2%. T5: 5%. T6: heat-treated 30 minutes. T7: 60 fed starter feed period 1 15 days, finisher 16 days until end experiment age 35 days. results showed that possibility meal in without affecting some biochemical blood characteristics immune chickens 308.

Language: Английский

Citations

0
Sustainable utilization of feather waste to produce alkaline serine protease by genetically engineered Bacillus amyloliquefaciens DOI

Ziyue Zhao,

Zhou Li, Ailing Guo

et al.

Journal of Cleaner Production, Journal Year: 2023, Volume and Issue: 434, P. 140138 - 140138

Published: Dec. 18, 2023

Language: Английский

Citations

8
Effect of a novel alkaline protease from Bacillus licheniformis on growth performance, carcass characteristics, meat quality, antioxidant capacity, and intestinal morphology of white feather broilers DOI

Wuzhou Yi,

Yanjie Liu,

Shijun Fu

et al.

Journal of the Science of Food and Agriculture, Journal Year: 2024, Volume and Issue: 104(9), P. 5176 - 5185

Published: Jan. 29, 2024

The present study was conducted to investigate the effects of dietary novel alkaline protease from Bacillus licheniformis on growth performance, meat quality, antioxidant status and intestinal morphology broilers. In total, 4000 broilers were randomly assigned into five groups treated with normal control, control + 100 mg kg

Language: Английский

Citations

2
Current Progress and Biotechnological Applications of Microbial Keratinases DOI Creative Commons
Indira Gahatraj, Anupom Borah, Piyush Pandey

et al.

Journal of Pure and Applied Microbiology, Journal Year: 2023, Volume and Issue: 17(2), P. 732 - 748

Published: May 31, 2023

Keratin is a fibrous and recalcitrant protein found in feathers, nails, horns, hooves, the epidermis of skin. The presence high degree disulfide bonds, hydrogen hydrophobic interactions makes them resistant to mechanical stress are not degraded by common proteases such as trypsin, pepsin, papain. Due slow degradation keratinous protein, accumulation solid wastes from poultry, slaughterhouse, textile, leather industries leads waste problems other environmental health related problems. In this review, efficient biodegradation microorganisms, low-cost, environmentally friendly strategy has been discussed. Keratinases microbial hydrolyze hard keratin. decomposition keratin keratinases maintains original structure final products, including short peptides, amino acids, organic nitrogen which deteriorated when traditional or chemical method implemented. article, role producing bacterial fungal species their attributes elaborated, along with biochemical characteristics keratinases, further, engineering approaches discussed, prospects enhance activity for biotechnological applications.

Language: Английский

Citations

5
Transcriptome Sequencing and Mass Spectrometry Reveal Genes Involved in the Non-mendelian Inheritance-Mediated Feather Growth Rate in Chicken DOI Creative Commons
Mohan Qiu, Zengrong Zhang,

Shiliang Zhu

et al.

Biochemical Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 27, 2024

Abstract The feather growth rate in chickens included early and late feathering. We attempted to characterize the genes pathways associated with that are not agreement Mendelian inheritance. Gene expression profiles hair follicle tissues of late-feathering cocks (LC), early-feathering (EC), hens (LH), (EH) were acquired using RNA sequencing (RNA-seq), mass spectrometry (MS), quantitative reverse transcription PCR (qRT‑PCR). A total 188 differentially expressed (DEGs) ascertained EC vs. LC 538 DEGs identified EH LH. observed 14 up-regulated 9 down-regulated screened both MS revealed 41 138 proteins (DEPs) out LH, respectively. Moreover, these DEPs enriched multiple feather-related pathways, including JAK-STAT, MAPK, WNT, TGF-β, calcium signaling pathways. qRT-PCR assay showed WNT8A was decreased compared EC, while ALK GRM4 significantly relative This study helps elucidate potential mechanism do conform genetic law.

Language: Английский

Citations

1