The role of bacterial extracellular vesicles in promoting antibiotic resistance DOI
Shaoqi Qu, Yanfang Zhang,

Lihui Weng

et al.

Critical Reviews in Microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 18

Published: Nov. 4, 2024

The burgeoning proliferation of infections attributed to multidrug-resistant (MDR) bacterial pathogens is profoundly undermining conventional chemotherapeutic modalities, portending a grave menace global public health. propagation drug resistance among bacteria fundamentally facilitated by interactions, with extracellular vesicles (EVs) assuming critical role in interbacterial communication. Here, we briefly delineate the methodologies for isolation, extraction, and characterization EVs from both Gram-negative Gram-positive origins. We further investigate assorted augment EV production, embracing physical stimulation, chemical elicitation, genetic engineering. Moreover, expound on pivotal involvement facilitation anticipate future trajectories research application potential. This overview EV-mediated novel mechanisms horizontal gene transfer implicated antibiotic aims obstruct transmission conduits thus fortify health integrity.

Language: Английский

Assessing microbiota in vivo: debugging with medical imaging DOI
Donna E. Goldhawk, Kait F. Al,

Sarah C. Donnelly

et al.

Trends in Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Proteome analysis of outer membrane vesicles from Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease DOI
Lihan Wang, Qifan Zeng, Jingjie Hu

et al.

Journal of Invertebrate Pathology, Journal Year: 2024, Volume and Issue: 204, P. 108082 - 108082

Published: March 5, 2024

Language: Английский

Citations

3
Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display DOI Creative Commons
Dávid Szöllősi, Polett Hajdrik, Hedvig Tordai

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Oct. 31, 2023

The important roles of bacterial outer membrane vesicles (OMVs) in various diseases and their emergence as a promising platform for vaccine development targeted drug delivery necessitates the imaging techniques suitable quantifying biodistribution with high precision. To address this requirement, we aimed to develop an OMV specific radiolabeling technique positron emission tomography (PET). A novel strain (E. coli BL21(DE3) ΔnlpI, ΔlpxM) was created efficient production, OMVs were characterized using methods. SpyCatcher anchored autotransporter-based surface display systems. Synthetic SpyTag-NODAGA conjugates tested binding 64Cu labeling efficiency. final protocol shows radiochemical purity 100% ~ 29% efficiency excellent serum stability. vivo labeled determined mice PET/MRI which revealed that radiolabeled is characteristic previously reported data highest organ uptakes corresponding liver spleen 3, 6, 12 h following intravenous administration. This method can serve basis general scheme could be used vaccine- drug-carrier based on bioengineered OMVs.

Language: Английский

Citations

9
Quantitative Biodistribution of OMVs Using SPECT/CT Imaging with HYNIC-Duramycin Radiolabeling DOI Creative Commons
Dávid Szöllősi, Polett Hajdrik, Hedvig Tordai

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(42), P. 42808 - 42813

Published: Oct. 11, 2024

Bacterial outer membrane vesicles (OMVs) are emerging as important players in the host-microbiome interaction, while also proving to be a promising platform for vaccine development and targeted drug delivery. The available methods measuring their biodistribution, however, limited. We aimed establish high-efficiency radiolabeling method treatment of OMVs.

Language: Английский

Citations

1
The role of bacterial extracellular vesicles in promoting antibiotic resistance DOI
Shaoqi Qu, Yanfang Zhang,

Lihui Weng

et al.

Critical Reviews in Microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 18

Published: Nov. 4, 2024

The burgeoning proliferation of infections attributed to multidrug-resistant (MDR) bacterial pathogens is profoundly undermining conventional chemotherapeutic modalities, portending a grave menace global public health. propagation drug resistance among bacteria fundamentally facilitated by interactions, with extracellular vesicles (EVs) assuming critical role in interbacterial communication. Here, we briefly delineate the methodologies for isolation, extraction, and characterization EVs from both Gram-negative Gram-positive origins. We further investigate assorted augment EV production, embracing physical stimulation, chemical elicitation, genetic engineering. Moreover, expound on pivotal involvement facilitation anticipate future trajectories research application potential. This overview EV-mediated novel mechanisms horizontal gene transfer implicated antibiotic aims obstruct transmission conduits thus fortify health integrity.

Language: Английский

Citations

1