Establishment of a Steatosis Model in LMH Cells, Chicken Embryo Hepatocytes, and Liver Tissues Based on a Mixture of Sodium Oleate and Palmitic Acid DOI Creative Commons

Wuchao Zhuang,

Ziwei Chen,

Xin Shu

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(15), P. 2173 - 2173

Published: July 26, 2024

Research on hepatic steatosis in animal husbandry has been a prominent area of study. Developing an appropriate vitro cellular model is crucial for comprehensively investigating the mechanisms involved liver lipid deposition poultry and identifying potential interventions to address abnormalities metabolism. The research methods chickens, particularly effects different fat mixtures, still lacking. In this study, LMH cells were utilized investigate OA, SO, PA, SP, their pairwise combinations development, with aim optimal conditions inducing steatosis. Analysis triglyceride (TG) content revealed that OA SP had limited efficacy increasing TG content, while combination SO PA 1:2 ratio exhibited highest content. Moreover, Oil Red O staining results demonstrated treatment more pronounced induction effect compared 0.375 mM SO. Additionally, RNA-seq analysis showed significantly influenced expression genes associated fatty acid metabolism control group, whereas led enrichment key GO terms programmed cell death. These findings suggest varying could lead distinct disruptions gene expression. also tested chicken embryonic embryos. detection assays successfully induced However, pattern differed from cells. This study lays foundations further investigations into avian

Language: Английский

The role of short-chain fatty acid in metabolic syndrome and its complications: focusing on immunity and inflammation DOI Creative Commons
Wenqian Yu,

Siyuan Sun,

Qiang Fu

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 7, 2025

Metabolic syndrome (Mets) is an important contributor to morbidity and mortality in cardiovascular, liver, neurological, reproductive diseases. Short-chain fatty acid (SCFA), organismal energy donor, has recently been demonstrated increasing number of studies be molecule ameliorating immuno-inflammation, causative factor Mets, improve lipid distribution, blood glucose, body weight levels animal models Mets. This study reviews recent research advances on SCFA Mets from immune-inflammatory perspective, including complications dominated by chronic inflammation, as well the fact that these findings also contribute understanding specific mechanisms which gut flora metabolites metabolic processes humans. review proposes emerging role for inflammatory followed identification major ambiguities further understand anti-inflammatory potential this substance In addition, novel strategies modulate treatment may help mitigate prognosis its complications.

Language: Английский

Citations

4
Maternal high-fat diet regulates offspring hepatic ABCG5 expression and cholesterol metabolism via the gut microbiota and its derived butyrate DOI Creative Commons
Ling Zhang, Shixuan Zhang,

Wenyu Zou

et al.

Clinical Science, Journal Year: 2024, Volume and Issue: 138(17), P. 1039 - 1054

Published: Aug. 13, 2024

Maternal high-fat diet intake has profound effects on the long-term health of offspring, predisposing them to a higher susceptibility obesity and metabolic dysfunction-associated steatotic liver disease. However, detailed mechanisms underlying role maternal in hepatic lipid accumulation especially at weaning age, remain largely unclear. In this study, female C57BL/6J mice were randomly assigned either or control diet, metabolism parameters assessed male offspring weaning. Gut microbiota analysis targeted metabolomics short-chain fatty acids (SCFAs) these further performed. Both vivo vitro studies conducted explore butyrate cholesterol excretion HepG2 cells. Our results showed that feeding led dyslipidemia, exacerbated livers We observed significant decreases abundance Firmicutes phylum Allobaculum genus, known as producers SCFAs, particularly butyrate, dams fed diet. Additionally, markedly decreased serum levels down-regulated ATP-binding cassette transporters G5 (ABCG5) liver, accompanied by phosphorylated AMP-activated protein kinase (AMPK) histone deacetylase 5 (HADC5) expressions. Subsequent revealed could induce ABCG5 activation alleviate via AMPK-pHDAC5 pathway Moreover, knockdown HDAC5 up-regulated expression promoted conclusion, our study provides novel insights into how inhibits down-regulates through butyrate-AMPK-pHDAC5

Language: Английский

Citations

5
Butyric acid-based products, alone or in combination with hydroxy-selenomethionine, improve performance of laying hens in post-peak period by modulating their antioxidant, metabolic and immune status DOI Creative Commons
Zhiyuan Xia,

Guoxin Gong,

Ronghui Huang

et al.

Poultry Science, Journal Year: 2025, Volume and Issue: 104(4), P. 104840 - 104840

Published: Jan. 22, 2025

This study aimed to evaluate the effects of butyrate, butyric glycerides (BG) and their combination with sodium selenite (SeNa) or hydroxy-selenomethionine (OH-SeMet) on performance egg quality laying hens in post-peak period as well potential mechanism. A total 900 45-week-old Hy-Line brown were randomly allocated 5 treatment groups (n = 10 replicates/diet, 18 hens/replicate). The fed basal diet supplemented 0.3 mg/kg selenium from SeNa (Control), Control plus 240 acid coated butyrate (CB), glycerides, OH-SeMet (CB+OH-SeMet) (BG+OH-SeMet), respectively, for 20 weeks. Serum, liver, isthmus, uterus, jejunum collected at end trial biochemistry, histology, redox status, gene expression analysis. Compared Control, diets BG, CB+OH-SeMet BG+OH-SeMet increased (p < 0.05) average weight (0.6-2.2 %), while only (7.1 %) egg-laying rate (4.6 decreased feed/egg ratio (5.0 throughout whole experiment. Furthermore, reduced content IL-6 alanine aminotransferase (15.4-32.5 elevated IgA, IgY, IgM protein (18.7-26.8 serum comparison Control. Notably, dietary supplementation performed more effective antioxidant capacity decreasing malondialdehyde (16.4-27.9 increasing activity glutathione peroxidase (17.6-36.3 various tissues. Further experiment revealed that regulated lipid metabolism by Carnitine palmitoyltransferase 1A (CPT1A) Lipoprotein lipase (LPL) liver. In conclusion, BG could improve via enhancement regulation metabolism.

Language: Английский

Citations

0
Steam explosion modified pea peptides alleviates hepatosteatosis by regulating lipid metabolism pathways and promoting autophagy DOI
Jingjing Zhang, Lianjun Song, Tiange Li

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 208, P. 116182 - 116182

Published: March 14, 2025

Language: Английский

Citations

0
The lotus seed starch-EGCG complex modulates obesity in C57BL/6J mice through the regulation of the gut microbiota DOI
Lu Liu,

Ru Jia,

Wenjing Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143256 - 143256

Published: April 17, 2025

Language: Английский

Citations

0
Sodium butyrate alleviates T‐2 toxin–induced liver toxicity and renal toxicity in quails by modulating oxidative stress–related Nrf2 signaling pathway, inflammation, and CYP450 enzyme system DOI
Yihao He,

Xueyan Zhu,

Huanni Song

et al.

Journal of Food Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 3, 2024

Abstract T‐2 toxin is a member of class A aspergilloides toxins, one the most prevalent mycotoxins that contaminate feed and food. Direct ingestion animals or contaminated by can cause various animal diseases. Butyrate an organic fatty acid featuring four‐carbon chain, which commonly found in form sodium butyrate (NaB). NaB has several biological functions pharmacological effects. However, role alleviating toxin–induced hepatorenal toxicity not been explored. In this study, 240 juvenile quails were evenly assigned into 4 groups. The experimental setup comprised four groups: control group received standard diet; diet containing 0.9 mg/kg toxin; 0.5 g/kg NaB; treatment both NaB. We evaluated histopathological changes kidney liver on Days 14 28 explored molecular mechanisms involving oxidative stress, inflammation, expression nuclear xenobiotic receptors (NXRs). Our results showed exposure–induced inflammation activating stress pathway modulating NXRs to regulate related CYP450 isoforms, ultimately leading injury kidney, whereas ameliorated injury. These offer novel insights underlying protective effects mitigating quails. Practical Application have well described animals, but studies birds are limited. With development society, market scale farming expanding, value meat eggs increasing; there urgent need clarify harm its mechanism.

Language: Английский

Citations

2
Establishment of a Steatosis Model in LMH Cells, Chicken Embryo Hepatocytes, and Liver Tissues Based on a Mixture of Sodium Oleate and Palmitic Acid DOI Creative Commons

Wuchao Zhuang,

Ziwei Chen,

Xin Shu

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(15), P. 2173 - 2173

Published: July 26, 2024

Research on hepatic steatosis in animal husbandry has been a prominent area of study. Developing an appropriate vitro cellular model is crucial for comprehensively investigating the mechanisms involved liver lipid deposition poultry and identifying potential interventions to address abnormalities metabolism. The research methods chickens, particularly effects different fat mixtures, still lacking. In this study, LMH cells were utilized investigate OA, SO, PA, SP, their pairwise combinations development, with aim optimal conditions inducing steatosis. Analysis triglyceride (TG) content revealed that OA SP had limited efficacy increasing TG content, while combination SO PA 1:2 ratio exhibited highest content. Moreover, Oil Red O staining results demonstrated treatment more pronounced induction effect compared 0.375 mM SO. Additionally, RNA-seq analysis showed significantly influenced expression genes associated fatty acid metabolism control group, whereas led enrichment key GO terms programmed cell death. These findings suggest varying could lead distinct disruptions gene expression. also tested chicken embryonic embryos. detection assays successfully induced However, pattern differed from cells. This study lays foundations further investigations into avian

Language: Английский

Citations

1