Multi-omics analyses reveal that Sirtuin 5 promotes the development of pre-recruitment follicles by inhibiting the autophagy-lysosome pathway in chicken granulosa cells

Poultry Science, Journal Year: 2025, Volume and Issue: 104(3), P. 104884 - 104884

Published: Feb. 7, 2025

The development of pre-recruitment follicles plays a critical role in determining egg-laying performance poultry. This study combines proteomic and metabolomic analyses to explore changes proteins metabolites, elucidate key regulatory mechanism involved chicken follicular development. Histological examination revealed significant increase yolk deposition small yellow (SYF) compared white (SWF). Metabolomics analysis identified significantly enriched differential metabolites (DMs) between SWF SYF pathways such as Lysosome, Ferroptosis, Biosynthesis unsaturated fatty acids, Tryptophan metabolism. Particularly, Adenosine-5'-Diphosphate (ADP) was downregulated during recruitment the lysosome pathway. Proteomic that differentially expressed (DEPs) were including Glutathione metabolism, Cholesterol Arginine proline amino acid biosynthesis. Among these DEPs, NAD-dependent protein deacetylase sirtuin 5 (SIRT5) upregulated, while lysosomal-associated membrane 1 (LAMP1) down-regulated follicles. SIRT5 linked negative regulation reactive oxygen species whereas LAMP1 associated with autophagy pathways. Further validation experiments demonstrated high expression SYF, particularly granulosa cells (GCs). Silencing GCs resulted increased ROS production upregulated autophagy-related LC3Ⅱ Beclin1, well markers LAMP1. Conversely, lipid droplet p62 suppressed. inhibited. Taken together, findings suggest upregulation promotes by inhibiting autophagy-lysosome pathway GCs.

Language: Английский

Quercetin mitigates iron-induced cell death in chicken granulosa cell

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 8, 2024

Abstract Background Granulosa cell (GC) apoptosis, ferroptosis, and other programmed death processes are markers of follicular aging. Quercetin has been shown to reduce however, its effects on ferroptosis in poultry remains unexplored. Our preliminary study identified aging ovaries. Therefore, the present study, 540-day-old Mountain Plum-blossom chickens were fed with quercetin supplementation at varying doses (0.2, 0.4, 0.6 g/kg), examined molecular GC using an vitro Erastin-induced model. Results The results showed that significantly increased egg production, which confirmed potential alleviate chicken ovarian tissue. experiment revealed Fer-1 (positive control) mitigated GCs. Further, transcriptome analysis modulated key genes such as acyl-CoA synthetase long-chain family member 4 ( ACSL4 ), solute carrier 7 11 SLC7A11 transferrin receptor TFRC involved regulation. further also reduced apoptosis inflammation by modulating expression proteins related inflammatory factors (NF-κB, TNF-α, IL-6, IL-10). Conclusion Taken together, improves production performance mitigates hens, inhibits inflammation, These findings protective role tissue cellular mechanisms against detrimental production. Graphical

Language: Английский

Preliminary construction of non-coding RNAs and ceRNA regulatory networks mediated by exosomes in porcine follicular fluid

Genomics, Journal Year: 2024, Volume and Issue: 116(5), P. 110920 - 110920

Published: Aug. 14, 2024

Follicles are fundamental units of the ovary, regulated intricately during development. Exosomes and ovarian granulosa cells (OGCs) play pivotal roles in follicular development, yet regulatory mechanisms governing exosomes remain elusive.

Language: Английский