International Journal of Food Engineering,
Journal Year:
2025,
Volume and Issue:
21(5), P. 285 - 303
Published: May 1, 2025
Abstract
Lipase
has
emerged
as
a
promising
eco-friendly
biocatalyst.
However,
its
free-form
application
is
limited
by
challenges,
such
poor
recovery
and
stability.
Immobilization
presents
an
effective
strategy
to
overcome
these
limitations,
thereby
enhancing
catalytic
performance.
Nevertheless,
lipase
immobilization
not
obstacle-free
either
necessitates
comprehensive
solutions.
The
development
of
novel
techniques
matrix
materials
remains
relatively
underexplored.
This
review
offers
analysis
both
traditional
advanced
enzyme
strategies,
with
focus
on
how
different
carrier
influence
enzymatic
properties
efficiency.
By
discussing
the
diverse
industrial
applications
immobilized
lipases
while
also
outlining
potential
research
trajectories
within
this
domain,
aims
establish
robust
theoretical
framework
for
improving
performance
enzymes.
Furthermore,
innovative
technologies
3D
printing
membrane-based
are
expected
drive
future
advancements,
providing
strong
foundation
engineering
lipases.
Chemosphere,
Journal Year:
2024,
Volume and Issue:
355, P. 141749 - 141749
Published: March 21, 2024
Plastic
pollution
has
become
a
major
global
concern,
posing
numerous
challenges
for
the
environment
and
wildlife.
Most
conventional
ways
of
plastics
degradation
are
inefficient
cause
great
damage
to
ecosystems.
The
development
biodegradable
offers
promising
solution
waste
management.
These
designed
break
down
under
various
conditions,
opening
up
new
possibilities
mitigate
negative
impact
traditional
plastics.
Microbes,
including
bacteria
fungi,
play
crucial
role
in
bioplastics
by
producing
secreting
extracellular
enzymes,
such
as
cutinase,
lipases,
proteases.
However,
these
microbial
enzymes
sensitive
extreme
environmental
temperature
acidity,
affecting
their
functions
stability.
To
address
challenges,
scientists
have
employed
protein
engineering
immobilization
techniques
enhance
enzyme
stability
predict
structures.
Strategies
improving
substrate
interaction,
increasing
thermostability,
reinforcing
bonding
between
active
site
substrate,
refining
activity
being
utilized
boost
functionality.
Recently,
bioengineering
through
gene
cloning
expression
potential
microorganisms,
revolutionized
biodegradation
bioplastics.
This
review
aimed
discuss
most
recent
strategies
modifying
bioplastic-degrading
terms
functionality,
thermostability
enhancement,
binding
site,
with
other
improvement
surface
action.
Additionally,
discovered
exoenzymes
metagenomics
were
emphasized.
Fermentation,
Journal Year:
2023,
Volume and Issue:
9(7), P. 581 - 581
Published: June 21, 2023
This
paper
aims
to
present
the
advances
related
biotechnological
application
of
lipases
Y.
lipolytica,
presenting
their
properties
and
more
efficient
ways
use
them
in
different
industrial
applications.
Waste
treatment
bioremediation
highlight
recent
studies
interest
large-scale
applications
food
sector
biofuel
production.
The
USA
China,
two
major
world
powers
industy,
are
utmost
importance
search
for
improvement
development
a
controlled
system
production
significant
number
lipase
from
lipolytica.
Chemosensors,
Journal Year:
2023,
Volume and Issue:
11(7), P. 388 - 388
Published: July 12, 2023
Neurotransmitters
(NTs)
play
a
crucial
role
in
regulating
the
behavioral
and
physiological
functions
of
nervous
system.
Imbalances
concentrations
NT
have
been
directly
linked
to
various
neurological
diseases
(e.g.,
Parkinson’s,
Huntington’s,
Alzheimer’s
disease),
addition
multiple
psychotic
disorders
such
as
schizophrenia,
depression,
dementia,
other
neurodegenerative
disorders.
Hence,
rapid
real-time
monitoring
NTs
is
utmost
importance
comprehending
identifying
Among
different
sensing
techniques,
electrochemical
biosensors
garnered
significant
interest
due
their
ability
deliver
fast
results,
compatibility
for
miniaturization
portability,
high
sensitivity,
good
controllability.
Furthermore,
utilization
enzymes
recognition
elements
biosensing
design
has
renewed
attention
unique
advantages
catalytic
biorecognition
coupled
with
simultaneous
signal
amplification.
This
review
paper
primarily
focuses
on
covering
recent
advances
enzymatic
detection
NTs,
encompassing
sensors,
electrode
materials,
electroanalytical
techniques.
Moreover,
we
shed
light
applications
enzyme-based
complex
matrices
vivo
monitoring.
Despite
numerous
biosensors,
there
are
still
challenges
that
need
be
addressed,
which
thoroughly
discussed
this
paper.
Finally,
also
presents
an
outlook
future
perspectives
opportunities
development
detection.
Critical Reviews in Food Science and Nutrition,
Journal Year:
2022,
Volume and Issue:
63(24), P. 6653 - 6686
Published: Feb. 18, 2022
Microbial
lipase
is
looking
for
better
attention
with
the
fast
growth
of
enzyme
proficiency
and
other
benefits
like
easy,
cost-effective,
reliable
manufacturing.
Immobilized
enzymes
can
be
used
repetitively
are
incapable
to
catalyze
reactions
in
system
continuously.
Hydrophobic
supports
utilized
immobilize
when
ionic
strength
low.
This
approach
allows
immobilization,
purification,
stability,
hyperactivation
lipases
a
single
step.
The
diffusion
substrate
more
advantageous
on
hydrophobic
than
hydrophilic
carrier.
These
approaches
critical
immobilization
performance
enzyme.
For
synthesis
provides
higher
pH
value
as
well
greater
heat
stability.
Using
mixture
methods,
binding
force
between
support
rises,
reducing
leakage.
Lipase
adsorption
produces
interfacial
activation
it
immobilized
support.
As
result,
process,
this
procedure
primarily
variety
industrial
applications.
sources,
techniques,
applications
fields
food,
flavor,
detergent,
paper
pulp,
pharmaceuticals,
biodiesel,
derivatives
esters
amino
groups,
agrochemicals,
biosensor
applications,
cosmetics,
perfumery,
bioremediation
all
discussed
review.
Advanced NanoBiomed Research,
Journal Year:
2022,
Volume and Issue:
3(2)
Published: Dec. 23, 2022
Diabetic
wound,
also
known
as
diabetic
foot
ulcer
(DFU),
is
a
form
of
chronic
lesion
that
disrupts
the
patient's
lifestyle
and,
in
severe
cases,
may
be
fatal.
Recent
endeavors
utilizing
enzymes,
particularly
nanozymes
conjunction
with
other
materials,
have
revealed
potential
to
treat
wounds.
Herein,
rational
structural
design
enzyme‐based
biomaterials
summarized
after
presenting
types
and
processes
enzymes
employed
wound
healing,
goal
better
understanding
interaction
between
biological
materials.
The
importance
enzyme
catalytic
activity,
coupled
new
therapeutic
treatment
strategies,
emphasized
use
for
healing.
This
review
represents
most
recent
development
innovation
healing
applications,
it
can
used
reference
guide
regenerative
biomedicines
from
nanoenzyme
biomaterials.
