Immobilization technology of lipase and application progress research DOI
Jiaxin Song, Fan Wu, Sichen Tao

et al.

International Journal of Food Engineering, Journal Year: 2025, Volume and Issue: 21(5), P. 285 - 303

Published: May 1, 2025

Abstract Lipase has emerged as a promising eco-friendly biocatalyst. However, its free-form application is limited by challenges, such poor recovery and stability. Immobilization presents an effective strategy to overcome these limitations, thereby enhancing catalytic performance. Nevertheless, lipase immobilization not obstacle-free either necessitates comprehensive solutions. The development of novel techniques matrix materials remains relatively underexplored. This review offers analysis both traditional advanced enzyme strategies, with focus on how different carrier influence enzymatic properties efficiency. By discussing the diverse industrial applications immobilized lipases while also outlining potential research trajectories within this domain, aims establish robust theoretical framework for improving performance enzymes. Furthermore, innovative technologies 3D printing membrane-based are expected drive future advancements, providing strong foundation engineering lipases.

Language: Английский

Advances in microbial exoenzymes bioengineering for improvement of bioplastics degradation DOI Creative Commons
Farzad Rahmati, Debadatta Sethi, Weixi Shu

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 355, P. 141749 - 141749

Published: March 21, 2024

Plastic pollution has become a major global concern, posing numerous challenges for the environment and wildlife. Most conventional ways of plastics degradation are inefficient cause great damage to ecosystems. The development biodegradable offers promising solution waste management. These designed break down under various conditions, opening up new possibilities mitigate negative impact traditional plastics. Microbes, including bacteria fungi, play crucial role in bioplastics by producing secreting extracellular enzymes, such as cutinase, lipases, proteases. However, these microbial enzymes sensitive extreme environmental temperature acidity, affecting their functions stability. To address challenges, scientists have employed protein engineering immobilization techniques enhance enzyme stability predict structures. Strategies improving substrate interaction, increasing thermostability, reinforcing bonding between active site substrate, refining activity being utilized boost functionality. Recently, bioengineering through gene cloning expression potential microorganisms, revolutionized biodegradation bioplastics. This review aimed discuss most recent strategies modifying bioplastic-degrading terms functionality, thermostability enhancement, binding site, with other improvement surface action. Additionally, discovered exoenzymes metagenomics were emphasized.

Language: Английский

Citations

26

A Review on Lipases: Sources, Assays, Immobilization Techniques on Nanomaterials and Applications DOI
Yosri A. Fahim, Ahmed M. El‐Khawaga, Reem M. Sallam

et al.

BioNanoScience, Journal Year: 2024, Volume and Issue: 14(2), P. 1780 - 1797

Published: Feb. 17, 2024

Language: Английский

Citations

22

Advances in the application of immobilized enzyme for the remediation of hazardous pollutant: A review DOI

P.R. Yaashikaa,

M. Keerthana Devi,

P. Senthil Kumar

et al.

Chemosphere, Journal Year: 2022, Volume and Issue: 299, P. 134390 - 134390

Published: March 23, 2022

Language: Английский

Citations

55

Lipase from Yarrowia lipolytica: Prospects as an Industrial Biocatalyst for Biotechnological Applications DOI Creative Commons
Jessica Lopes da Silva, Misael Bessa Sales, Viviane de Castro Bizerra

et al.

Fermentation, Journal Year: 2023, Volume and Issue: 9(7), P. 581 - 581

Published: June 21, 2023

This paper aims to present the advances related biotechnological application of lipases Y. lipolytica, presenting their properties and more efficient ways use them in different industrial applications. Waste treatment bioremediation highlight recent studies interest large-scale applications food sector biofuel production. The USA China, two major world powers industy, are utmost importance search for improvement development a controlled system production significant number lipase from lipolytica.

Language: Английский

Citations

30

Smart chemistry and applied perceptions of enzyme-coupled nano-engineered assemblies to meet future biocatalytic challenges DOI
Ayesha Anwar, Muhammad Imran, Hafiz M.N. Iqbal

et al.

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 493, P. 215329 - 215329

Published: July 9, 2023

Language: Английский

Citations

28

Enzymatic Electrochemical Biosensors for Neurotransmitters Detection: Recent Achievements and Trends DOI Creative Commons
Zina Fredj, Baljit Singh, Mohamed Bahri

et al.

Chemosensors, Journal Year: 2023, Volume and Issue: 11(7), P. 388 - 388

Published: July 12, 2023

Neurotransmitters (NTs) play a crucial role in regulating the behavioral and physiological functions of nervous system. Imbalances concentrations NT have been directly linked to various neurological diseases (e.g., Parkinson’s, Huntington’s, Alzheimer’s disease), addition multiple psychotic disorders such as schizophrenia, depression, dementia, other neurodegenerative disorders. Hence, rapid real-time monitoring NTs is utmost importance comprehending identifying Among different sensing techniques, electrochemical biosensors garnered significant interest due their ability deliver fast results, compatibility for miniaturization portability, high sensitivity, good controllability. Furthermore, utilization enzymes recognition elements biosensing design has renewed attention unique advantages catalytic biorecognition coupled with simultaneous signal amplification. This review paper primarily focuses on covering recent advances enzymatic detection NTs, encompassing sensors, electrode materials, electroanalytical techniques. Moreover, we shed light applications enzyme-based complex matrices vivo monitoring. Despite numerous biosensors, there are still challenges that need be addressed, which thoroughly discussed this paper. Finally, also presents an outlook future perspectives opportunities development detection.

Language: Английский

Citations

27

Cross-linked enzyme aggregates of polyethylene terephthalate hydrolyse (PETase) from Ideonella sakaiensis for the improvement of plastic degradation DOI
Yi Lin Lee,

Nardiah Rizwana Jaafar,

Jonathan Guyang Ling

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 263, P. 130284 - 130284

Published: Feb. 19, 2024

Language: Английский

Citations

10

One-step immobilization of chitosanase on microcrystalline cellulose using a carbohydrate binding module family 2 DOI

Qijun Yu,

Jinshui Yang, Liang Liu

et al.

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: 353, P. 123291 - 123291

Published: Jan. 19, 2025

Language: Английский

Citations

1

Sources, purification, immobilization and industrial applications of microbial lipases: An overview DOI

Enespa,

Prem Chandra, D. P. Singh

et al.

Critical Reviews in Food Science and Nutrition, Journal Year: 2022, Volume and Issue: 63(24), P. 6653 - 6686

Published: Feb. 18, 2022

Microbial lipase is looking for better attention with the fast growth of enzyme proficiency and other benefits like easy, cost-effective, reliable manufacturing. Immobilized enzymes can be used repetitively are incapable to catalyze reactions in system continuously. Hydrophobic supports utilized immobilize when ionic strength low. This approach allows immobilization, purification, stability, hyperactivation lipases a single step. The diffusion substrate more advantageous on hydrophobic than hydrophilic carrier. These approaches critical immobilization performance enzyme. For synthesis provides higher pH value as well greater heat stability. Using mixture methods, binding force between support rises, reducing leakage. Lipase adsorption produces interfacial activation it immobilized support. As result, process, this procedure primarily variety industrial applications. sources, techniques, applications fields food, flavor, detergent, paper pulp, pharmaceuticals, biodiesel, derivatives esters amino groups, agrochemicals, biosensor applications, cosmetics, perfumery, bioremediation all discussed review.

Language: Английский

Citations

39

Recent Advances in Enzyme‐Based Biomaterials Toward Diabetic Wound Healing DOI Creative Commons
Qi Wang, Zheng Luo, Yun‐Long Wu

et al.

Advanced NanoBiomed Research, Journal Year: 2022, Volume and Issue: 3(2)

Published: Dec. 23, 2022

Diabetic wound, also known as diabetic foot ulcer (DFU), is a form of chronic lesion that disrupts the patient's lifestyle and, in severe cases, may be fatal. Recent endeavors utilizing enzymes, particularly nanozymes conjunction with other materials, have revealed potential to treat wounds. Herein, rational structural design enzyme‐based biomaterials summarized after presenting types and processes enzymes employed wound healing, goal better understanding interaction between biological materials. The importance enzyme catalytic activity, coupled new therapeutic treatment strategies, emphasized use for healing. This review represents most recent development innovation healing applications, it can used reference guide regenerative biomedicines from nanoenzyme biomaterials.

Language: Английский

Citations

30