Investigative Ophthalmology & Visual Science,
Journal Year:
2025,
Volume and Issue:
66(1), P. 50 - 50
Published: Jan. 22, 2025
Purpose:
The
purpose
of
this
study
was
to
investigate
the
activated
core
kinases
involved
in
DNA
damage
responses
(DDR)
during
ferroptosis
retinal
pigment
epithelial
(RPE)
cells
vitro
and
their
regulatory
effects
on
ferroptosis.
Methods:
Ferroptosis
induced
by
erastin
RPE
(iRPE)
derived
from
human
umbilical
cord
mesenchymal
stem
(hUCMSCs),
hUCMSCs,
pluripotent
cell-derived
(iPSC-RPE)
cells.
CCK8
employed
measure
cell
viability.
Calcein/PI
staining
used
detect
ferroptotic
γ-H2AX,
8-oxoG,
phosphorylated
DNA-dependent
protein
kinase
catalytic
subunit
(DNA-PKcs)
were
determined
through
immunostaining.
phosphorylation
DNA-PKcs
ERK1/2
Western
blotting.
Lipid
peroxides
detected
BODIPY581/591-C11
staining.
Results:
iRPE
exhibited
a
stronger
ability
resist
compared
hUCMSCs.
cells,
rapidly
treatment
erastin.
In
addition,
inhibition
promoted
suggesting
that
prevents
Meanwhile,
inhibited
only
at
early
stage
induction,
whereas
played
protective
role
Furthermore,
inducing
its
promoting
also
verified
iPSC-RPE
Conclusions:
present
elucidates
key
DDR
is
plays
vitro,
which
will
provide
new
research
targets
strategies
for
inhibiting
ACS Nano,
Journal Year:
2023,
Volume and Issue:
17(17), P. 17199 - 17216
Published: Aug. 25, 2023
The
clinical
applications
of
currently
used
photosensitizers
are
limited
by
high
costs,
inconvenient
preparation,
suboptimal
biodegradability,
and
a
lack
biological
activity.
Humic
acids
(HAs)
show
photothermal
activity
can
be
as
photosensitizer
for
therapy.
In
the
presence
various
functional
groups,
HAs
endowed
with
anti-inflammatory
antioxidant
activities.
solubility
is
dependent
on
pH
value,
which
soluble
in
neutral
to
alkaline
conditions
undergoes
conformational
change
coiled
compact
structure
acidic
conditions.
Additionally,
Cu2+
an
emerging
therapeutic
agent
cutaneous
wounds
chelated
form
complexes.
this
study,
we
explore
ability
modulate
inflammatory
response,
particularly
macrophage
polarization,
potential
underlying
mechanism.
We
fabricate
near-infrared
(NIR)/pH
dual-responsive
Cu-HAs
nanoparticle
(NP)-based
poly(vinyl
alcohol)
(PVA)
hydrogel
film
loaded
SEW2871
(SEW),
recruitment
agent,
treat
bacteria-infected
wounds.
results
that
could
promote
M2
polarization
dose-dependent
manner.
NPs
successfully
eradicated
bacterial
infection
through
NIR-induced
local
hyperthermia.
This
PVA@Cu-HAs
NPs@SEW
improves
tissue
regeneration
promoting
alleviating
oxidative
stress,
enhancing
angiogenesis,
facilitating
collagen
deposition.
These
findings
highlight
treatment
bacterially
infected
wound
healing.
MedComm,
Journal Year:
2024,
Volume and Issue:
5(8)
Published: Aug. 1, 2024
Abstract
Macrophages
are
versatile
immune
cells
with
remarkable
plasticity,
enabling
them
to
adapt
diverse
tissue
microenvironments
and
perform
various
functions.
Traditionally
categorized
into
classically
activated
(M1)
alternatively
(M2)
phenotypes,
recent
advances
have
revealed
a
spectrum
of
macrophage
activation
states
that
extend
beyond
this
dichotomy.
The
complex
interplay
signaling
pathways,
transcriptional
regulators,
epigenetic
modifications
orchestrates
polarization,
allowing
respond
stimuli
dynamically.
Here,
we
provide
comprehensive
overview
the
cascades
governing
focusing
on
roles
Toll‐like
receptors,
signal
transducer
activator
transcription
proteins,
nuclear
microRNAs.
We
also
discuss
emerging
concepts
metabolic
reprogramming
trained
immunity,
contributing
their
functional
adaptability.
Macrophage
plasticity
plays
pivotal
role
in
repair
regeneration,
macrophages
coordinating
inflammation,
angiogenesis,
matrix
remodeling
restore
homeostasis.
By
harnessing
potential
novel
therapeutic
strategies
targeting
polarization
could
be
developed
for
diseases,
including
chronic
wounds,
fibrotic
disorders,
inflammatory
conditions.
Ultimately,
deeper
understanding
molecular
mechanisms
underpinning
will
pave
way
innovative
regenerative
medicine
engineering
approaches.
Signal Transduction and Targeted Therapy,
Journal Year:
2024,
Volume and Issue:
9(1)
Published: Oct. 9, 2024
Metabolism,
including
glycolysis,
oxidative
phosphorylation,
fatty
acid
oxidation,
and
other
metabolic
pathways,
impacts
the
phenotypes
functions
of
immune
cells.
The
regulation
system
is
important
in
pathogenesis
progression
numerous
diseases,
such
as
cancers,
autoimmune
diseases
diseases.
concept
immunometabolism
was
introduced
over
a
decade
ago
to
elucidate
intricate
interplay
between
metabolism
immunity.
definition
has
expanded
from
chronic
low-grade
inflammation
reprogramming
cells
various
With
being
proposed
developed,
can
be
gradually
summarized
becomes
more
clearer.
In
context
many
cancer,
disease,
occurs
inducing
proinflammatory
or
anti-inflammatory
effects.
phenotypic
functional
changes
caused
by
further
affect
development
Based
on
experimental
results,
targeting
cellular
promising
therapy.
this
review,
we
focus
introduce
their
pathways
reprogramming,
summarize
how
these
effects
We
thoroughly
explore
targets
treatments
based
existing
studies.
challenges
translating
results
into
clinical
applications
field
are
also
summarized.
believe
that
better
understanding
health
will
improve
management
most
Biomarker Research,
Journal Year:
2025,
Volume and Issue:
13(1)
Published: Jan. 23, 2025
Neutrophil
extracellular
traps
(NETs)
are
intricate,
web-like
formations
composed
of
DNA,
histones,
and
antimicrobial
proteins,
released
by
neutrophils.
These
structures
participate
in
a
wide
array
physiological
pathological
activities,
including
immune
rheumatic
diseases
damage
to
target
organs.
Recently,
the
connection
between
NETs
cancer
has
garnered
significant
attention.
Within
tumor
microenvironment
metabolism,
exhibit
multifaceted
roles,
such
as
promoting
proliferation
migration
cells,
influencing
redox
balance,
triggering
angiogenesis,
driving
metabolic
reprogramming.
This
review
offers
comprehensive
analysis
link
emphasizing
areas
that
remain
underexplored.
include
interaction
with
mitochondria,
their
effect
on
states
within
tumors,
involvement
reprogramming,
contribution
angiogenesis
tumors.
Such
insights
lay
theoretical
foundation
for
deeper
understanding
role
development.
Moreover,
also
delves
into
potential
therapeutic
strategies
suggests
future
research
directions,
offering
new
perspectives
treatment
other
related
diseases.
Cancer Science,
Journal Year:
2023,
Volume and Issue:
114(6), P. 2306 - 2317
Published: Feb. 16, 2023
Tumor-associated
macrophages
(TAMs)
are
one
of
the
most
abundant
immunosuppressive
cells
in
tumor
microenvironment
and
possess
crucial
functions
facilitating
progression.
Emerging
evidence
indicates
that
altered
metabolic
properties
cancer
support
tumorigenic
TAMs.
However,
mechanisms
mediators
underly
cross-talk
between
TAMs
remain
largely
unknown.
In
present
study,
we
revealed
high
solute
carrier
family
3
member
2
(SLC3A2)
expression
lung
patients
was
associated
with
poor
prognosis.
Knockdown
SLC3A2
adenocarcinoma
impaired
M2
polarization
a
coculture
system.
Using
metabolome
analysis,
identified
knockdown
metabolism
changed
multiple
metabolites,
including
arachidonic
acid,
microenvironment.
More
importantly,
showed
acid
responsible
for
SLC3A2-mediated
macrophage
to
differentiate
into
type
both
vitro
vivo.
Our
data
illustrate
previously
undescribed
TAM
suggest
acts
as
switch
on
induce
phenotypic
reprogramming
through
acid.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(17), P. 13052 - 13052
Published: Aug. 22, 2023
Reactive
oxygen
species
(ROS)
are
important
regulating
factors
that
play
a
dual
role
in
plant
and
human
cells.
As
the
first
messenger
response
organisms,
ROS
coordinate
signals
growth,
development,
metabolic
activity
pathways.
They
also
can
act
as
an
alarm
mechanism,
triggering
cellular
responses
to
harmful
stimuli.
However,
excess
cause
oxidative
stress-related
damage
oxidize
organic
substances,
leading
malfunctions.
This
review
summarizes
current
research
status
mechanisms
of
eukaryotic
cells,
highlighting
differences
similarities
between
two
elucidating
their
interactions
with
other
reactive
substances
ROS.
Based
on
similar
regulatory
pathways
kingdoms,
this
proposes
future
developments
provide
opportunities
develop
novel
strategies
for
treating
diseases
or
creating
greater
agricultural
value.
The EMBO Journal,
Journal Year:
2024,
Volume and Issue:
43(4), P. 507 - 532
Published: Jan. 8, 2024
Abstract
Metabolic
syndrome
combines
major
risk
factors
for
cardiovascular
disease,
making
deeper
insight
into
its
pathogenesis
important.
We
here
explore
the
mechanistic
basis
of
metabolic
by
recruiting
an
essential
patient
cohort
and
performing
extensive
gene
expression
profiling.
The
mitochondrial
fatty
acid
metabolism
enzyme
acyl-CoA
synthetase
medium-chain
family
member
3
(ACSM3
)
was
identified
to
be
significantly
lower
expressed
in
peripheral
blood
patients.
In
line,
hepatic
ACSM3
decreased
mice
with
syndrome.
Furthermore,
Acsm3
knockout
showed
glucose
lipid
abnormalities,
accumulation
substrate
lauric
acid.
depletion
markedly
function
stimulated
signaling
via
p38
MAPK
pathway
cascade.
Consistently,
mouse
exhibited
abnormal
morphology,
ATP
contents,
enhanced
ROS
levels
their
livers.
Mechanistically,
deficiency,
activated
nuclear
receptor
Hnf4α-p38
signaling.
inhibitor
Adezmapimod
effectively
rescued
phenotype.
Together,
these
findings
show
that
disease-associated
loss
facilitates
dysfunction
a
acid-HNF4a-p38
axis,
suggesting
novel
therapeutic
vulnerability
systemic
dysfunction.
