Ferroptosis as a Potential Therapeutic Target for Reducing Inflammation and Corneal Scarring in Bacterial Keratitis DOI Creative Commons
Qiankun Chen, Leying Wang, Yuan Wei

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(2), P. 29 - 29

Published: Feb. 21, 2024

Purpose: Bacterial keratitis (BK) is a serious ocular infection that can cause severe inflammation and corneal scarring, leading to vision loss. In this study, we aimed investigate the involvement of ferroptosis in pathogenesis BK. Methods: Transcriptome analysis was performed evaluate ferroptosis-related gene expression human BK corneas. Subsequently, mouse models Pseudomonas aeruginosa stromal stem cells (CSSCs) were validated. The mice treated with levofloxacin (LEV) or combined ferrostatin-1 (LEV+Fer-1). CSSCs lipopolysaccharide (LPS) LPS Fer-1. Inflammatory cytokines, α-SMA, regulators evaluated by RT-qPCR, immunostaining, Western blot. Iron reactive oxygen species (ROS) measured. Results: revealed significant alterations genes models, group LEV+Fer-1 exhibited reduced inflammatory cytokines (MPO, TNF-α, IFN-γ), decreased scarring α-SMA expression, lower Fe3+ compared LEV groups. Notably, showed elevated GPX4 SLC7A11 contrast group. vitro, Fer-1 treatment effectively restored ROS, Fe2+, GPX4, induced CSSCs. Conclusions: Ferroptosis plays crucial role inhibition holds promise for mitigating inflammation, reducing ultimately enhancing prognosis Consequently, study provides potential target innovative therapeutic strategies BK, which immense transform

Language: Английский

Lycopene prevents Di-(2-ethylhexyl) phthalate-induced mitophagy and oxidative stress in mice heart via modulating mitochondrial homeostasis DOI
Yue Shen,

Lin Liu,

Muzi Li

et al.

The Journal of Nutritional Biochemistry, Journal Year: 2023, Volume and Issue: 115, P. 109285 - 109285

Published: Feb. 14, 2023

Language: Английский

Citations

42
Phthalates Induce Neurotoxicity by Disrupting the Mfn2-PERK Axis-Mediated Endoplasmic Reticulum–Mitochondria Interaction DOI
Yi Zhao, Yuan‐Hang Chang,

Hao-Ran Ren

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(13), P. 7411 - 7422

Published: Feb. 23, 2024

Di-(2-ethylhexyl) phthalate (DEHP), as the most common phthalate, has been extensively used a plasticizer to improve plasticity of agricultural products, which pose severe harm human health. Mitochondrial dynamics and endoplasmic reticulum (ER) homeostasis are indispensable for maintaining mitochondria-associated ER membrane (MAM) integrity. In this study, we aimed explore effect DEHP on nervous system its association with ER-mitochondria interaction. Here, showed that caused morphological changes, motor deficits, cognitive impairments, blood-brain barrier disruption in brain. triggered stress, is mainly mediated by protein kinase R-like (PERK) signaling. Moreover, DEHP-induced mitofusin-2 (Mfn2) downregulation results imbalance mitochondrial dynamics. Interestingly, exposure impaired MAMs inhibiting Mfn2-PERK Above all, study elucidates axis-mediated interaction phthalate-induced neurotoxicity could be potentially developed novel therapy neurological diseases.

Language: Английский

Citations

25
Co-exposure to molybdenum and cadmium evokes necroptosis and decreases apoptosis in duck myocardium DOI
Hang Liu, Xueyan Dai, Shiwen Xu

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 902, P. 166074 - 166074

Published: Aug. 6, 2023

Language: Английский

Citations

31
FTO-Nrf2 axis regulates bisphenol F-induced leydig cell toxicity in an m6A-YTHDF2-dependent manner DOI

Shimeng Zhou,

Jing-zhi Li,

Hong-qiang Chen

et al.

Environmental Pollution, Journal Year: 2023, Volume and Issue: 325, P. 121393 - 121393

Published: March 4, 2023

Language: Английский

Citations

25
Ferritinophagy Is Critical for Deoxynivalenol-Induced Liver Injury in Mice DOI
Junze Jiang,

Yongbao Ruan,

Xiaohui Liu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(12), P. 6660 - 6671

Published: March 19, 2024

Background: Deoxynivalenol (DON) contamination, pervasive throughout all stages of food production and processing, presents a significant threat to human health. The degradation ferritin mediated by nuclear receptor coactivator 4 (NCOA4), termed ferritinophagy, plays crucial role in maintaining iron homeostasis regulating ferroptosis. Aim: This study aims elucidate the ferritinophagy ferroptosis DON-induced liver injury. Methods: Male mice AML12 cells were subjected varying doses DON, serving as vivo vitro models, respectively. Protein expression was assessed using immunofluorescence western blot techniques. Co-immunoprecipitation employed investigate protein–protein interactions. Results: Our findings demonstrate that DON triggers hepatocyte ferritinophagy-dependent manner. Specifically, impedes activation mammalian target rapamycin complex 1 (mTORC1) inhibiting RAC1's binding mTOR, thereby ultimately inducing autophagy. Concurrently, amplifies NCOA4's affinity for facilitating NCOA4 phosphorylation through ataxia-telangiectasia mutated kinase (ATM), thus promoting autophagy-dependent ferritin. Both autophagy inhibition suppression ameliorate Conclusion: concludes facilitates NCOA4-mediated via ATM–NCOA4 pathway, subsequently liver.

Language: Английский

Citations

15
Cooperative application of transcriptomics and ceRNA hypothesis: lncRNA-00742/miR-116 targets CD74 to mediate vanadium-induced mitochondrial apoptosis in duck liver DOI
Jing Chen, Xueyan Dai, Chenghong Xing

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 135904 - 135904

Published: Sept. 19, 2024

Language: Английский

Citations

15
SPTBN2 suppresses ferroptosis in NSCLC cells by facilitating SLC7A11 membrane trafficking and localization DOI Creative Commons
Jun Deng, Xü Lin,

Jiajia Qin

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 70, P. 103039 - 103039

Published: Jan. 17, 2024

The function of SLC7A11 in the process ferroptosis is well-established, as it regulates synthesis glutathione (GSH), thereby influencing tumor development along with drug resistance non-small cell lung cancer (NSCLC). However, determinants governing SLC7A11's membrane trafficking and localization remain unknown. Our study identified SPTBN2 a suppressor, enhancing NSCLC cells to inducers. Mechanistically, SPTBN2, through its CH domain, interacted connected motor protein Arp1, thus facilitating — prerequisite for role System Xc−, which mediates cystine uptake GSH synthesis. Consequently, suppressed preserving functional activity Xc− on membrane. Moreover, Inhibiting increased sensitivity cisplatin induction, both vitro vivo. Using Abrine potential inhibitor, efficacy promoting sensitizing was validated. Collectively, therapeutic target addressing dysfunction NSCLC.

Language: Английский

Citations

13
AQP1 Deficiency Drives Phthalate-Induced Epithelial Barrier Disruption through Intestinal Inflammation DOI
Yi Zhao,

Zi-Yan Hu,

Ming Lou

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(27), P. 15334 - 15344

Published: June 25, 2024

Di-2-ethylhexyl phthalate (DEHP) is frequently used as a plasticizer to enhance the plasticity and durability of agricultural products, which pose adverse effects human health environment. Aquaporin 1 (AQP1) main water transport channel protein involved in maintenance intestinal integrity. However, impact DEHP exposure on gut its potential mechanisms remain elusive. Here, we determined that induced compromised duodenum structure, was concomitant with mitochondrial structural injury epithelial cells. Importantly, caused inflammatory cell damage strong response accompanied by activating TLR4/MyD88/NF-κB signaling pathway. Mechanistically, directly inhibits expression AQP1 thus leads an response, ultimately disrupting integrity barrier function. Collectively, our findings uncover role phthalate-induced disorders, could be promising therapeutic approach for treating patients disorders or diseases.

Language: Английский

Citations

12
Redox Homeostasis and Nrf2-Regulated Mechanisms Are Relevant to Male Infertility DOI Creative Commons
Cinzia Signorini, Luciano Saso, Somayyeh Ghareghomi

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(2), P. 193 - 193

Published: Feb. 3, 2024

Infertility represents a significant global health challenge, affecting more than 12% of couples worldwide, and most cases infertility are caused by male factors. Several pathological pathways implicated in infertility. The main mechanisms involved driven the loss reduction–oxidation (redox) homeostasis resulting oxidative damage as well chronic inflammatory process. Increased or severe stress leads to sperm plasma membrane DNA damage, dysregulated RNA processing, telomere destruction. signaling these molecular events also regulated Nuclear factor-E2-related factor 2 (Nrf2). causes infertility, role Keap1-Nrf2 antioxidant pathway reviewed. This review highlights regulatory Nrf2 balance between oxidants antioxidants relevant fertility. is regulation spermatogenesis quality. Establishing link fertility provides basis for modulation processes, reactive oxygen species generation, network, including Nrf2-regulated response, improve reproductive outcomes.

Language: Английский

Citations

11
SLC7A11 as a therapeutic target to attenuate phthalates-driven testosterone level decline in mice DOI Creative Commons
Yi Zhao, Xueqi Wang, Ruiqi Liu

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: May 1, 2024

Phthalates exposure is a major public health concern due to the accumulation in environment and associated with levels of testosterone reduction, leading adverse pregnancy outcomes. However, relationship between phthalate-induced level decline ferroptosis remains poorly defined.

Language: Английский

Citations

9