Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H2S Mediated Persulfidation DOI

Andrew T. Stoltzfus,

Jasper G. Ballot,

Thibaut Vignane

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(27)

Published: May 29, 2024

Abstract The gasotransmitter hydrogen sulfide (H 2 S) is thought to be involved in the post‐translational modification of cysteine residues produce reactive persulfides. A persulfide‐specific chemoselective proteomics approach with mammalian cells has identified a broad range zinc finger (ZF) proteins as targets persulfidation. Parallel studies isolated ZFs show that persulfidation mediated by Zn II , O and H S, intermediates involving oxygen‐ sulfur‐based radicals detected mass spectrometry optical spectroscopies. small molecule complex exhibits analogous reactivity S giving persulfidated product. These data not just biological structural element, but also plays critical role mediating S‐dependent ZF appears general possible conduit for signaling. This work implications our understanding S‐mediated signaling regulation cellular physiology development.

Language: Английский

Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H2S Mediated Persulfidation DOI

Andrew T. Stoltzfus,

Jasper G. Ballot,

Thibaut Vignane

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(27)

Published: May 29, 2024

The gasotransmitter hydrogen sulfide (H

Language: Английский

Citations

9
Neurobehavioral dysfunction in a mouse model of Down syndrome: upregulation of cystathionine β-synthase, H2S overproduction, altered protein persulfidation, synaptic dysfunction, endoplasmic reticulum stress, and autophagy DOI Creative Commons

Theodora Panagaki,

Lucia Janickova,

Dunja Petrovic

et al.

GeroScience, Journal Year: 2024, Volume and Issue: 46(5), P. 4275 - 4314

Published: April 1, 2024

Down syndrome (DS) is a genetic condition where the person born with an extra chromosome 21. DS associated accelerated aging; people are prone to age-related neurological conditions including early-onset Alzheimer's disease. Using Dp(17)3Yey/ + mice, which overexpresses portion of mouse 17, encodes for transsulfuration enzyme cystathionine β-synthase (CBS), we investigated functional role CBS/hydrogen sulfide (H

Language: Английский

Citations

8
Role of the sulfur-containing amino acid-ROS axis in cancer chemotherapeutic drug resistance DOI
Bingli Wu, Yinwei Cheng, Liyan Li

et al.

Drug Resistance Updates, Journal Year: 2025, Volume and Issue: unknown, P. 101238 - 101238

Published: March 1, 2025

Language: Английский

Citations

0
Hydrogen sulfide attenuates PM2.5-induced COPD by inhibiting cellular senescence via the Klotho/Parkin-mediated mitophagy signaling pathway DOI Creative Commons
Ying Wang,

Guoqing Han,

Jin Yang

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 293, P. 117987 - 117987

Published: March 1, 2025

Fine particulate matter (PM2.5), an atmospheric pollutant, plays a crucial role in mediating the occurrence of chronic obstructive pulmonary disease (COPD). Hydrogen sulfide (H2S) has shown therapeutic potential countering COPD, but underlying mechanisms remain elusive. This study aimed to elucidate H2S inhibiting cellular senescence and combating progression COPD. In this study, mouse cell models were initially established by pretreating with PM, PM2.5, NaHS. It was showed that increased expressions p16, p21, β-gal positive cells, along elevated inflammatory oxidative stress markers reduced antioxidant markers, could be observed COPD patients as well PM2.5-induced models, correlating decreased endogenous levels. Pre-treatment NaHS reversed these effects, enhancing Klotho expression, IGF-1R, activating Parkin- dependent mitophagy pathway, which significantly mitigated airway inflammation emphysema. silencing BEAS-2B cells exacerbated damage diminished protective effects NaHS, confirming link between downstream Parkin-dependent pathway. Our findings suggest mitigates epithelial modulating Klotho/IGF-1R/Parkin-dependent thereby alleviating provides foundation for devising innovative treatment approaches mitigating health pollution.

Language: Английский

Citations

0
Increased hydrogen sulfide turnover serves a cytoprotective role during the development of replicative senescence DOI
Anna Kieronska‐Rudek, Kelly Ascenção, Stefan Chłopicki

et al.

Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 116595 - 116595

Published: Oct. 1, 2024

Language: Английский

Citations

3
Hydrogen Sulfide (H2S- or H2Sn-Polysulfides) in Synaptic Plasticity: Modulation of NMDA Receptors and Neurotransmitter Release in Learning and Memory DOI Open Access
Constantin Munteanu, Anca‐Irina Galaction, Gelu Onose

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3131 - 3131

Published: March 28, 2025

Hydrogen sulfide (H2S) has emerged as a pivotal gaseous transmitter in the central nervous system, influencing synaptic plasticity, learning, and memory by modulating various molecular pathways. This review examines recent evidence regarding how H2S regulates NMDA receptor function neurotransmitter release neuronal circuits. By synthesizing findings from animal cellular models, we investigate impacts of enzymatic production exogenous on excitatory currents, long-term potentiation, intracellular calcium signaling. Data suggest that interacts directly with subunits, altering excitability. Simultaneously, promotes neurotransmitters such glutamate GABA, shaping dynamics plasticity. Furthermore, reports indicate disruptions metabolism contribute to cognitive impairments neurodegenerative disorders, underscoring potential therapeutic value targeting H2S-mediated Although precise mechanisms H2S-induced changes strength remain elusive, growing body positions significant regulator formation processes. calls for more rigorous exploration into underpinnings paving way novel pharmacological interventions dysfunction.

Language: Английский

Citations

0
Role of cystathionine-β-synthase and hydrogen sulfide in down syndrome DOI Creative Commons
Csaba Szabó

Neurotherapeutics, Journal Year: 2025, Volume and Issue: unknown, P. e00584 - e00584

Published: April 1, 2025

Language: Английский

Citations

0
Hydrogen Sulfide can Scavenge Free Radicals to Improve Spinal Cord Injury by Inhibiting the p38MAPK/mTOR/NF-κB Signaling Pathway DOI
Kexin Lin, Yong Zhang,

Yanyang Shen

et al.

NeuroMolecular Medicine, Journal Year: 2024, Volume and Issue: 26(1)

Published: June 21, 2024

Language: Английский

Citations

2
Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H2S Mediated Persulfidation DOI

Andrew T. Stoltzfus,

Jasper G. Ballot,

Thibaut Vignane

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(27)

Published: May 29, 2024

Abstract The gasotransmitter hydrogen sulfide (H 2 S) is thought to be involved in the post‐translational modification of cysteine residues produce reactive persulfides. A persulfide‐specific chemoselective proteomics approach with mammalian cells has identified a broad range zinc finger (ZF) proteins as targets persulfidation. Parallel studies isolated ZFs show that persulfidation mediated by Zn II , O and H S, intermediates involving oxygen‐ sulfur‐based radicals detected mass spectrometry optical spectroscopies. small molecule complex exhibits analogous reactivity S giving persulfidated product. These data not just biological structural element, but also plays critical role mediating S‐dependent ZF appears general possible conduit for signaling. This work implications our understanding S‐mediated signaling regulation cellular physiology development.

Language: Английский

Citations

0