LWT, Journal Year: 2024, Volume and Issue: 211, P. 116897 - 116897
Published: Oct. 19, 2024
Language: Английский
European Journal of Heart Failure, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 14, 2025
Aims Hyperglycaemic conditions increase cardiac stress, a common phenomenon associated with inflammation, aging, and metabolic imbalance. Sodium–glucose cotransporter 2 inhibitors, class of anti‐diabetic drugs, showed to improve cardiovascular functions although their mechanism action has not yet been fully established. This study investigated the effects empagliflozin on cardiomyocytes following high glucose exposure, specifically focusing inflammatory responses. Methods results A three‐part strategy was formulated: (i) meta‐analysis selected randomized clinical trials carried out assess anti‐inflammatory in diabetic patients; (ii) impact human cardiomyocyte AC16 cells exposed normal (5 mM) (33 concentrations for 7 days explored by evaluating gene expression protein levels pivotal markers endoplasmic reticulum damage, calcium modulation; (iii) silico data from bioinformatic analyses were exploited construct an interaction map delineating potential tissue. Empagliflozin reversed high‐glucose mediated alterations at transcriptional level, decreasing inflammatory, metabolic, aging signatures. Specifically, vitro experiments cardiomyocytes, meta‐analyses biomarkers peripheral blood samples, sequencing pathological heart tissues, all support that exerts both systemically directly tissue, cardiomyocytes. Conclusion Our provides insights based robust mechanistic optimizing failure management highlights intricate interplay between diabetes, health.
Language: Английский
Citations
1
Insects, Journal Year: 2024, Volume and Issue: 15(10), P. 797 - 797
Published: Oct. 14, 2024
Increased levels of reactive oxygen species (ROS) produced during aerobic metabolism in animals can negatively affect the intracellular redox status, cause oxidative stress and interfere with physiological processes cells. The antioxidant defence regulates ROS by interplaying diverse enzymes non-enzymatic metabolites. thioredoxin system, consisting enzyme reductase (TrxR), redox-active protein (Trx) NADPH, represent a crucial component defence. It is involved signalling regulation multiple developmental processes, such as cell proliferation or apoptotic death. Insects have evolved unique variations TrxR, which resemble mammalian overall structure catalytic mechanisms, but selenocysteine-cysteine pair active site replaced cysteine-cysteine typical bacteria. Moreover, role system insects indispensable due to absence glutathione reductase, an essential system. However, functions Trx are still poorly characterised. In present review, we provide critical overview current knowledge on insect focusing mainly TrxR's immune model species.
Language: Английский
Citations
4
Life Sciences, Journal Year: 2025, Volume and Issue: 369, P. 123531 - 123531
Published: March 5, 2025
Language: Английский
Citations
0
Archives of Toxicology, Journal Year: 2025, Volume and Issue: unknown
Published: April 9, 2025
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: April 11, 2025
Ischemia-reperfusion injury refers to the damage that occurs when blood supply is restored organs or tissues after a period of ischemia. This phenomenon commonly observed in clinical contexts such as organ transplantation and cardiac arrest resuscitation. Among these, hepatic ischemia-reperfusion prevalent complication liver transplantation, significantly impacting functional recovery transplanted potentially leading primary graft dysfunction. With growing demand for transplants limited availability donor organs, effectively addressing essential enhancing success rates, minimizing complications, improving survival. The pathogenesis multifaceted, involving factors oxidative stress inflammatory responses. article focuses on role protein post-translational modifications injury, including phosphorylation, ubiquitination, acetylation, ADP-ribosylation, SUMOylation, crotonylation, palmitoylation, S-nitrosylation. Initially, we examined historical discovery these subsequently investigated their impact cellular signal transduction, enzymatic activity, stability, protein-protein interactions. emphasis this study pivotal progression potential therapeutic targets. aims conduct comprehensive analysis recent advancements research investigate underlying molecular mechanisms, explore future trajectories. Additionally, directions are proposed, exploration interactions between various modifications, identification specific modification sites, development drugs targeting modifications. These efforts aim deepen our understanding pave way innovative interventions.
Language: Английский
Citations
0
ACS Central Science, Journal Year: 2025, Volume and Issue: unknown
Published: April 13, 2025
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: May 21, 2025
Protein sulfenylation (protein-SOH) is a central oxidation product of protein post-translational modification (PTM) that crucial for signal transduction and cell behavior. However, the natural properties protein-SOH, especially its low responsiveness dynamic reversibility, pose great challenge to development chemical probes visualize protein-SOH in vivo. Here, we report an activated aggregation-induced emission (AIE) probe specifically lighting-up The AIE-active reacts with by nucleophilic substitution exhibits intense fluorescence due restriction intramolecular motion. uniqueness this ensures only lighted up avoiding interference from small-molecule active substances nonspecific adsorption proteins. significant increase atherosclerotic mice detected AIE probe, level positively correlates atherosclerosis progression. Significantly, find specific binding can inhibit plaque development, making it promising therapeutic target. This study enables real-time imaging vivo, opening universal tool further elucidation PTM role transduction.
Language: Английский
Citations
0
LWT, Journal Year: 2024, Volume and Issue: 211, P. 116897 - 116897
Published: Oct. 19, 2024
Language: Английский
Citations
3