Recent advances in the biocatalytic mitigation of emerging pollutants: A comprehensive review

Journal of Biotechnology, Journal Year: 2023, Volume and Issue: 369, P. 14 - 34

Published: May 10, 2023

Language: Английский

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Investigating Biomolecules in Deep Eutectic Solvents with Molecular Dynamics Simulations: Current State, Challenges and Future Perspectives

Molecules, Journal Year: 2024, Volume and Issue: 29(3), P. 703 - 703

Published: Feb. 2, 2024

Deep eutectic solvents (DESs) have recently gained increased attention for their potential in biotechnological applications. DESs are binary mixtures often consisting of a hydrogen bond acceptor and donor, which allows tailoring properties particular If produced from sustainable resources, they can provide greener alternative to many traditional organic usage various applications (e.g., as reaction environment, crystallization agent, or storage medium). To navigate this large design space, it is crucial comprehend the behavior biomolecules enzymes, proteins, cofactors, DNA) impact individual components. Molecular dynamics (MD) simulations offer powerful tool understanding thermodynamic transport processes at atomic level insights into fundamental phenomena, may not be accessible through experiments. While experimental investigation well progressed, thorough via MD has only popularity recent years. Within work, we aim an overview current state modeling with discuss future directions focus optimizing molecular increasing our knowledge.

Language: Английский

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Biomimetic mineralization of lipase@MOF biocatalyst for ease of biodiesel synthesis: Structural insights into the catalytic behavior

Fuel, Journal Year: 2023, Volume and Issue: 357, P. 129854 - 129854

Published: Sept. 19, 2023

Language: Английский

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Bioremediation of organic pollutants by laccase-metal–organic framework composites: A review of current knowledge and future perspective

Bioresource Technology, Journal Year: 2024, Volume and Issue: 406, P. 131072 - 131072

Published: July 4, 2024

Language: Английский

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Determination of anticancer activity and biosynthesis of Cu, Zn, and Co hybrid nanoflowers with Tribulus terrestris L. extract

Process Biochemistry, Journal Year: 2024, Volume and Issue: 138, P. 14 - 22

Published: Jan. 12, 2024

Language: Английский

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Synthesis and application of mesoporous MIL-88A for enhanced lipase immobilization and high value oil conversion

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 496, P. 153802 - 153802

Published: July 6, 2024

Language: Английский

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Efficient synthesis of isoamyl acetate in deep eutectic solvent utilizing in-situ aqueous phase immobilized lipase biocatalyst

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 105963 - 105963

Published: Jan. 1, 2025

Language: Английский

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Efficient biodiesel production from waste cooking oil by fast co-immobilization of lipases from Aspergillus oryzae and Rhizomucor miehei in magnetic chitosan microcapsules

Process Biochemistry, Journal Year: 2022, Volume and Issue: 125, P. 171 - 180

Published: Dec. 24, 2022

Language: Английский

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Co-Immobilization of Lipases with Different Specificities for Efficient and Recyclable Biodiesel Production from Waste Oils: Optimization Using Response Surface Methodology

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(5), P. 4726 - 4726

Published: March 1, 2023

Lipase-catalyzed transesterification is a promising and sustainable approach to producing biodiesel. To achieve highly efficient conversion of heterogeneous oils, combining the specificities advantages different lipases an attractive strategy. this end, active Thermomyces lanuginosus lipase (1,3-specific) stable Burkholderia cepacia (non-specific) were covalently co-immobilized on 3-glycidyloxypropyltrimethoxysilane (3-GPTMS) modified Fe3O4 magnetic nanoparticles (co-BCL-TLL@Fe3O4). The co-immobilization process was optimized using response surface methodology (RSM). obtained co-BCL-TLL@Fe3O4 exhibited significant improvement in activity reaction rate compared with mono combined-use lipases, achieving 92.9% yield after 6 h under optimal conditions, while individually immobilized TLL, BCL their combinations yields 63.3%, 74.2% 70.6%, respectively. Notably, achieved 90–98% biodiesel 12 six feedstocks, demonstrating perfect synergistic effect TLL remarkably motivated co-immobilization. Furthermore, could maintain 77% initial nine cycles by removing methanol glycerol from catalyst surface, accomplished washing t-butanol. high catalytic efficiency, wide substrate adaptability favorable reusability suggest that it will be economical effective biocatalyst for further applications.

Language: Английский

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Chitosan‐Based metal-organic framework for Stabilization of β-glucosidase: Reusability and storage stability

Heliyon, Journal Year: 2023, Volume and Issue: 9(11), P. e21169 - e21169

Published: Oct. 18, 2023

Enzyme immobilization is a powerful tool for protecting enzymes from harsh reaction conditions and improving enzyme activity, stability, reusability. In this study, metal organic frameworks (MIL-Fe composites) were synthesized via solvothermal reactions then modified with chitosan (CS). β-Glucosidase was immobilized on the chitosan-metal framework (CS-MIL-Fe), resulting composites characterized various analytical techniques. The β-glucosidase CS-MIL-Fe composite had an yield of 85 % recovered activity 74 %. retained 81 its initial after ten successive cycles preserved 69 original 30 days storage at 4 °C. contrast, free only 32 days. Under temperature pH conditions, showed greater stability than enzyme, optimal 60 °C 6.0 50 5.0 enzyme. kinetic parameters also determined, Km values 13.4 6.98 mM β-glucosidase, respectively, Vmax 3.96 1.72 U/mL, respectively. Overall, these results demonstrate that CS-MIL-Fe@β-glucosidase promising matrix showing high catalytic efficiency enhanced stability.

Language: Английский

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