Defining the networks that connect RNase III and RNase J-mediated regulation of primary and specialized metabolism in Streptomyces venezuelae

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

ABSTRACT RNA metabolism involves coordinating synthesis with processing and degradation. Ribonucleases play fundamental roles within the cell, contributing to cleavage, modification, degradation of molecules, these actions ensuring appropriate gene regulation cellular homeostasis. Here, we employed RNA-sequencing explore impact RNase III J on transcriptome Streptomyces venezuelae . Differential expression analysis comparing wild type mutant strains at distinct developmental stages revealed significant changes in transcript abundance, particularly pathways related multicellular development, nutrient acquisition, specialized metabolism. Both mutants exhibited dysregulation BldD regulon, including altered many cyclic-di-GMP-associated enzymes. We also observed precocious chloramphenicol production found that mutant, this was associated PhoP-mediated regulation. further directly targeted members PhoP suggesting a link between regulator bridges primary connected function translation through observation targets multiple ribosomal protein transcripts for These findings establish distinct, but complementary dynamics critical S. development

Language: Английский

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Defining the networks that connect RNase III and RNase J-mediated regulation of primary and specialized metabolism in Streptomyces venezuelae

Journal of Bacteriology, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

ABSTRACT RNA metabolism involves coordinating synthesis with processing and degradation. Ribonucleases play fundamental roles within the cell, contributing to cleavage, modification, degradation of molecules, these actions ensuring appropriate gene regulation cellular homeostasis. Here, we employed sequencing explore impact RNase III J on transcriptome Streptomyces venezuelae . Differential expression analysis comparing wild-type mutant strains at distinct developmental stages revealed significant changes in transcript abundance, particularly pathways related multicellular development, nutrient acquisition, specialized metabolism. Both mutants exhibited dysregulation BldD regulon, including altered many cyclic-di-GMP-associated enzymes. We also observed precocious chloramphenicol production found that mutant, this was associated PhoP-mediated regulation. further directly targeted members PhoP suggesting a link between regulator bridges primary connected function translation through observation targets multiple ribosomal protein transcripts for These findings establish but complementary dynamics critical S. development IMPORTANCE are mediated by ribonucleases processes all cells. In morphologically complex metabolically sophisticated bacteria, influence both poorly understood mechanisms. show required proper pathway contribute control production, an interesting connection phosphate III. Additionally, RNases have potential mechanisms can cooperatively degrading specific transcripts. This study advances our understanding biology providing insight into contributions made enzymes intriguing interplay them.

Language: Английский

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Natural product discovery in soil actinomycetes: unlocking their potential within an ecological context

Current Opinion in Microbiology, Journal Year: 2024, Volume and Issue: 79, P. 102487 - 102487

Published: May 11, 2024

Natural products (NPs) produced by bacteria, particularly soil actinomycetes, often possess diverse bioactivities and play a crucial role in human health, agriculture, biotechnology. Soil actinomycete genomes contain vast number of predicted biosynthetic gene clusters (BGCs) yet to be exploited. Understanding the factors governing NP production an ecological context activating cryptic silent BGCs actinomycetes will provide researchers with wealth molecules potential novel applications. Here, we highlight recent advances discovery strategies employing ecology-inspired approaches discuss importance understanding environmental signals responsible for activation production, microbial community context, as well challenges that remain.

Language: Английский

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