Functional characterisation of Dof gene family and expression analysis under abiotic stresses and melatonin-mediated tolerance in pitaya (Selenicereus undatus)

Functional Plant Biology, Journal Year: 2024, Volume and Issue: 51(4)

Published: April 3, 2024

DNA binding proteins with one finger (Dof) transcription factors are essential for seed development and defence against various biotic abiotic stresses in plants. Genomic analysis of Dof has not been determined yet pitaya (Selenicereus undatus). In this study, we have identified 26 gene family members, renamed as HuDof-1 to HuDof-26, clustered them into seven subfamilies based on conserved motifs, domains, phylogenetic analysis. The pairs members were duplicated by segmental duplications that faced purifying selection, indicated the Ka/Ks ratio values. Promoter regions HuDof genes contain many cis-acting elements related phytohormones including abscisic acid, jasmonic gibberellin, temperature, light. We exposed plants different environmental examined melatonin’s influence expression levels. Signifcant HuDof-2 HuDof-6 observed developmental stages flower buds, flowers, pericarp, pulp. Pitaya subjected stresses, transcriptome was carried out identify role members. RNA-sequencing data reverse quantitative PCR-based revealed three putative candidate (HuDof-1, HuDof-2, HuDof-8), which might diverse roles stresses. Our study provides a theoretical foundation functional through traditional modern biotechnological tools trait improvement.

Language: Английский

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Functional characterization of MATE gene family under abiotic stresses and melatonin-mediated tolerance in dragon fruit (Selenicereus undatus L.)

Plant Stress, Journal Year: 2023, Volume and Issue: 11, P. 100300 - 100300

Published: Nov. 22, 2023

Multidrug and toxic compound extrusion (MATE) are transporter proteins exists widely in all living organisms which involved toxins detoxification. In plants, MATE functions detoxification of endogenous secondary metabolites, exogenous agents, other plant developmental processes. this study, the identification expression analysis gene family was conducted to analyse response against heavy metal salt stresses pitaya seedlings. We have identified analysed 35 from genome were mapped on 11 chromosomes. All members named HuMATE-1 HuMATE-35, divided into 14 groups based phylogenetic analysis, tree topology motif's structure. The subcellular localization predicted data showed that 62% total localized plasma membrane. syntenic collinearity pairs two tandem duplication twelve segmental Genes motif composition exon-intron structures found more similar within same group. Cis-acting element promoter regions their regulatory function toxin defence RNA-Seq HuMATE candidate genes exhibits higher under copper individually as well both combination. Melatonin applications regulate effectively for stresses, thus enhancing seedling growth development. Moreover, RT-qPCR highly expressed 10 at different stages validates results. Our finding predicts (HuMATE-1/7/8/10/11/12/13/16/28/35) may play a key role produced by metals accumulation high soil salinity. Furthermore, our results provide foundation development stress-tolerant genotypes various climate scenarios through forward reverse genetic breeding programs.

Language: Английский

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Comprehensive characterization of SBP genes revealed their role under multifactorial stress combinations in dragon fruit (Selenicereus undatus L.)

Plant Stress, Journal Year: 2023, Volume and Issue: 10, P. 100294 - 100294

Published: Nov. 16, 2023

The SQUAMOSA PROMOTER BINDING PROTEIN (SBP) gene family is essential for plant growth and development. However, little known about the SBP in pitaya (Selenicereus undatus L.); a fruit crop cultivated tropical climates, severely affected by abiotic stress factors. These stresses produce excessive reactive oxygen species (ROS), affecting In this study, we identified 17 genes genome (HuSBP) classified them into seven distinct clusters based on their phylogeny. HuSBP exhibited conserved intron-exon structure motif domain organization. were differentially expressed 15 developmental stages of flower (F), buds (FB), pericarp (PeriC), pulp. addition, specific higher expression under single-factor, double-factor, multifactorial stresses, revealing diverse role development pitaya. Exogenous application melatonin with antioxidant stress-protective properties, was also applied. pattern RNA-Seq data validated RT-qPCR analysis. Based genome-wide results transcriptome-based predicted five putative candidate (HuSBP1/3/7/11/14) that might play roles contain vital enzymes production phenylpropanoid glutathione metabolism. provide valuable insights tolerance could be used to develop innovative strategies create resilient adaptable genotypes using conventional breeding modern biotechnological tools.

Language: Английский

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Electromagnetic Field-Assisted Frozen Tissue Planarization Enhances MALDI-MSI in Plant Spatial Omics

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(29), P. 11809 - 11822

Published: July 8, 2024

Plant samples with irregular morphology are challenging for longitudinal tissue sectioning. This has restricted the ability to gain insight into some plants using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). Herein, we develop a novel technique termed electromagnetic field-assisted frozen planarization (EMFAFTP). involves pair of adjustable electromagnets on both sides plant tissue. Under an optimized field strength, nondestructive and regularization is induced, allowing sectioning that favors subsequent molecular profiling by MALDI-MSI. As proof concept, flowers, leaves roots from six species chosen evaluate performance EMFAFTP MALDI-MSI secondary metabolites, amino acids, lipids, proteins among others in samples. The significantly enhanced capabilities these endogenous molecules demonstrate robustness suggest it potential become standard advancing new era spatial omics.

Language: Английский

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Comparative omics-based characterization, phylogeny and melatonin-mediated expression analyses of GDSL genes in pitaya (Selenicereus undatus L.) against multifactorial abiotic stresses

Physiology and Molecular Biology of Plants, Journal Year: 2024, Volume and Issue: 30(9), P. 1493 - 1515

Published: Sept. 1, 2024

Language: Английский

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