Functional characterisation of Dof gene family and expression analysis under abiotic stresses and melatonin-mediated tolerance in pitaya (Selenicereus undatus)

Functional Plant Biology, Journal Year: 2024, Volume and Issue: 51(4)

Published: April 3, 2024

DNA binding proteins with one finger (Dof) transcription factors are essential for seed development and defence against various biotic abiotic stresses in plants. Genomic analysis of Dof has not been determined yet pitaya (Selenicereus undatus). In this study, we have identified 26 gene family members, renamed as HuDof-1 to HuDof-26, clustered them into seven subfamilies based on conserved motifs, domains, phylogenetic analysis. The pairs members were duplicated by segmental duplications that faced purifying selection, indicated the Ka/Ks ratio values. Promoter regions HuDof genes contain many cis-acting elements related phytohormones including abscisic acid, jasmonic gibberellin, temperature, light. We exposed plants different environmental examined melatonin’s influence expression levels. Signifcant HuDof-2 HuDof-6 observed developmental stages flower buds, flowers, pericarp, pulp. Pitaya subjected stresses, transcriptome was carried out identify role members. RNA-sequencing data reverse quantitative PCR-based revealed three putative candidate (HuDof-1, HuDof-2, HuDof-8), which might diverse roles stresses. Our study provides a theoretical foundation functional through traditional modern biotechnological tools trait improvement.

Language: Английский

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Omics-assisted crop improvement under abiotic stress conditions

Plant Stress, Journal Year: 2024, Volume and Issue: unknown, P. 100626 - 100626

Published: Oct. 1, 2024

Language: Английский

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Functional characterization of MATE gene family under abiotic stresses and melatonin-mediated tolerance in dragon fruit (Selenicereus undatus L.)

Plant Stress, Journal Year: 2023, Volume and Issue: 11, P. 100300 - 100300

Published: Nov. 22, 2023

Multidrug and toxic compound extrusion (MATE) are transporter proteins exists widely in all living organisms which involved toxins detoxification. In plants, MATE functions detoxification of endogenous secondary metabolites, exogenous agents, other plant developmental processes. this study, the identification expression analysis gene family was conducted to analyse response against heavy metal salt stresses pitaya seedlings. We have identified analysed 35 from genome were mapped on 11 chromosomes. All members named HuMATE-1 HuMATE-35, divided into 14 groups based phylogenetic analysis, tree topology motif's structure. The subcellular localization predicted data showed that 62% total localized plasma membrane. syntenic collinearity pairs two tandem duplication twelve segmental Genes motif composition exon-intron structures found more similar within same group. Cis-acting element promoter regions their regulatory function toxin defence RNA-Seq HuMATE candidate genes exhibits higher under copper individually as well both combination. Melatonin applications regulate effectively for stresses, thus enhancing seedling growth development. Moreover, RT-qPCR highly expressed 10 at different stages validates results. Our finding predicts (HuMATE-1/7/8/10/11/12/13/16/28/35) may play a key role produced by metals accumulation high soil salinity. Furthermore, our results provide foundation development stress-tolerant genotypes various climate scenarios through forward reverse genetic breeding programs.

Language: Английский

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Transcriptome Profiles Reveal Key Regulatory Networks during Single and Multifactorial Stresses Coupled with Melatonin Treatment in Pitaya (Selenicereus undatus L.)

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8901 - 8901

Published: Aug. 15, 2024

In response to evolving climatic conditions, plants frequently confront multiple abiotic stresses, necessitating robust adaptive mechanisms. This study focuses on the responses of Selenicereus undatus L. both individual stresses (cadmium; Cd, salt; S, and drought; D) their combined applications, with an emphasis evaluating mitigating effects (M) melatonin. Through transcriptome analysis, this identifies significant gene expression changes regulatory network activations. The results show that stress decreases pitaya growth rates by 30%, reduces stem cladode development 40%, increases Cd uptake under single 50% 70%, respectively. Under enhanced activities H2O2, POD, CAT, APX, SOD elevated proline content indicate strong antioxidant defenses. We identified 141 common DEGs related tolerance, most which were AtCBP, ALA, CBP pathways. Interestingly, production genes signal transduction hormones, including abscisic acid auxin, was also significantly induced. Several calcium-dependent protein kinase regulated during M treatments. Functional enrichment analysis showed enriched metabolism, MAPK signaling, photosynthesis. addition, weighted co-expression (WGCNA) critical transcription factors (WRKYs, MYBs, bZIPs, bHLHs, NACs) associated activities, particularly within salmon module. provides morpho-physiological insights into pitaya’s suggests molecular breeding techniques enhance plant resistance.

Language: Английский

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Improving heat tolerance in betel palm (Areca catechu) by characterisation and expression analyses of heat shock proteins under thermal stress

Crop and Pasture Science, Journal Year: 2024, Volume and Issue: 75(9)

Published: Sept. 12, 2024

Context Heat shock proteins play a vital role in cellular homeostasis by protecting against various environmental stresses, which facilitates the survival of plants under unfavourable conditions. Aims We aimed to provide first comprehensive genomic and expression analysis HSP70 gene family betel palm (Areca catechu) elucidate its heat stress response. Methods Genomic revealed 34 putative genes distributed across 13 chromosomes. These were renamed AcatHSP70 classified into five subfamilies (A–E) based on phylogenetic analysis. are mostly localised chloroplast, cytoplasm, nucleus. Gene ontology that these involved stress. The duplication events only segmental duplications. subjected seedlings (2 years old) controlled conditions for 30 days at high, low, room temperatures analyses genes. Key results Expression eight candidate (AcatHSP70-3, AcatHSP70-13, AcatHSP70-22, AcatHSP70-19, AcatHSP70-21, AcatHSP70-24, AcatHSP70-25, AcatHSP70-26) showed significantly higher high-temperature AcatHSP70-5 low-temperature treatment, AcatHSP70-16 was responsive temperature treatment. Conclusion conclude majority crucial thermal conditions, respond as shown quantitative reverse transcription polymerase chain reaction Implications This characterisation provides novel insights protection mechanisms palms changing climates.

Language: Английский

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