In vitro or not in vitro: a short journey through a long history DOI Creative Commons
Kristina Rehberger, Christian Kropf, Helmut Segner

et al.

Environmental Sciences Europe, Journal Year: 2018, Volume and Issue: 30(1)

Published: June 25, 2018

The aim of ecotoxicology is to study toxic effects on constituents ecosystems, with the protection goal being populations and communities rather than individual organisms. In this ecosystem perspective, use in vitro methodologies measuring cellular subcellular endpoints at a first glance appears be odd. Nevertheless, more recently approaches gained momentum ecotoxicology. article, we will discuss important application domains methods One area assays replace, reduce, refine (3R) vivo tests. Research field has focused mainly cytotoxicity fish cells as non-animal alternative lethality test biotransformation part an testing strategy for bioaccumulation fish. Lessons learned from research include importance critical evaluation sensitivity, specificity exposure conditions assays, well availability appropriate vitro-in extrapolation models. addition classical 3R application, other screening prioritization chemical hazards, categorization chemicals according their modes action provision mechanistic information pathway-based prediction adverse outcomes. applications discussed essay may highlight potential technologies enhance environmental hazard assessment single complex mixtures reduced need animal testing.

Language: Английский

Assessing the Mixture Effects in In Vitro Bioassays of Chemicals Occurring in Small Agricultural Streams during Rain Events DOI
Peta A. Neale, Georg Braun, Werner Brack

et al.

Environmental Science & Technology, Journal Year: 2020, Volume and Issue: 54(13), P. 8280 - 8290

Published: June 5, 2020

Rain events may impact the chemical pollution burden in rivers. Forty-four small streams Germany were profiled during several rain for presence of 395 chemicals and five types mixture effects vitro bioassays (cytotoxicity; activation estrogen, aryl hydrocarbon, peroxisome proliferator-activated receptors; oxidative stress response). While these selected to cover a wide range agricultural impacts, addition expected pesticides, wastewater-derived typical street runoff detected. The unexpectedly high estrogenic many samples indicated by wastewater or overflow combined sewer systems. 128 water exhibited diversity effect patterns, even different at same site. detected 290 explained only fraction (<8%) measured effects. experimental designed mixtures that dominate consistent with predictions concentration within factor two 94% mixtures. Overall, was much higher than previously surface dry weather, often exceeding proposed effect-based trigger values.

Language: Английский

Citations

88
Towards a holistic and solution-oriented monitoring of chemical status of European water bodies: how to support the EU strategy for a non-toxic environment? DOI Creative Commons
Werner Brack, Beate I. Escher, E. Müller

et al.

Environmental Sciences Europe, Journal Year: 2018, Volume and Issue: 30(1)

Published: Sept. 4, 2018

The definition of priority substances (PS) according to the Water Framework Directive (WFD) helped remove many these chemicals from market and reduce their concentrations in European water bodies. However, it could not prevent that have been replaced by others with similar risks. Today, monitoring PS-based chemical status WFD covers only a tiny fraction toxic risks, extensively ignores mixture effects lacks incentives guidance for abatement. Thus, we suggest complement this purely status-related approach more holistic solution-oriented monitoring, which at same time helps provide links ecological status. Major elements include (1) advanced screening techniques supporting risk assessment unraveling source-related patterns complex mixtures, (2) effect-based detection groups establishment toxicity fingerprints, (3) effect-directed analysis drivers (4) translate toxicological fingerprints into footprints prioritization management measures. requirement contamination is supported significant advancement appropriate tools within last years. Non-target technology, basic understanding are available conceptually research but also increasingly find way practical monitoring. Substantial progress development, evaluation demonstration tools, example, SOLUTIONS project enhanced acceptability. Further advancement, integration demonstration, extensive data exchange closure remaining knowledge gaps suggested as high needs next future bridge gap between insufficient cost-efficient abatement

Language: Английский

Citations

86
The NORMAN Association and the European Partnership for Chemicals Risk Assessment (PARC): let’s cooperate! DOI Creative Commons
Valeria Dulio, Jan Koschorreck, Bert van Bavel

et al.

Environmental Sciences Europe, Journal Year: 2020, Volume and Issue: 32(1)

Published: July 20, 2020

Abstract The Partnership for Chemicals Risk Assessment (PARC) is currently under development as a joint research and innovation programme to strengthen the scientific basis chemical risk assessment in EU. plan bring assessors managers together with scientists accelerate method production of necessary data knowledge, facilitate transition next-generation evidence-based assessment, non-toxic environment European Green Deal. NORMAN Network an independent, well-established competent network more than 80 organisations field emerging substances has enormous potential contribute implementation PARC partnership. stands ready provide expert advice PARC, drawing on its long experience development, harmonisation testing advanced tools relation chemicals concern support Early Warning System unravel risks contaminants (CECs) close gap between regulatory processes. In this commentary we highlight developed by that consider most relevant supporting initiative: (i) space cutting-edge concern; (ii) collaborative framework improve quality comparability; (iii) analysis early warning system (iv) national thanks harnessing, combining sharing evidence expertise CECs. By extensive knowledge financial policy-related strengths initiative, large step towards goal can be taken.

Language: Английский

Citations

78
Tackling the toxics in plastics packaging DOI Creative Commons
Jane Muncke

PLoS Biology, Journal Year: 2021, Volume and Issue: 19(3), P. e3000961 - e3000961

Published: March 30, 2021

The widespread use of plastic packaging for storing, transporting, and conveniently preparing or serving foodstuffs is significantly contributing to the global pollution crisis. This has led many efforts directed toward amending packaging’s end life, such as recycling, alternative material approaches, like increasingly using paper food packaging. But these approaches often neglect critical issue chemical migration: When contacting foodstuffs, chemicals that are present in transfer into thus unwittingly become part human diet. Hazardous chemicals, endocrine disrupters, carcinogens, substances bioaccumulate, collectively referred “chemicals concern.” They can from food, together with other unknown toxicologically uncharacterized chemicals. scientifically undisputed makes a known, avoidable, source exposure synthetic, hazardous, untested Here, I discuss this highlight aspects need improvement, namely way assessed toxicity. Further, provide an outlook on how contamination could be addressed future. Robust innovations must attempt systemic change tackle migration integrates all existing knowledge.

Language: Английский

Citations

67
Advances in identifying and managing emerging contaminants in aquatic ecosystems: Analytical approaches, toxicity assessment, transformation pathways, environmental fate, and remediation strategies DOI

M. Mofijur,

M.M. Hasan, Shams Forruque Ahmed

et al.

Environmental Pollution, Journal Year: 2023, Volume and Issue: 341, P. 122889 - 122889

Published: Nov. 15, 2023

Language: Английский

Citations

36
Application of Effect-Based Methods to Water Quality Monitoring: Answering Frequently Asked Questions by Water Quality Managers, Regulators, and Policy Makers DOI
Peta A. Neale, Beate I. Escher, Milo L. de Baat

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(15), P. 6023 - 6032

Published: April 7, 2023

Effect-based methods (EBM) have great potential for water quality monitoring as they can detect the mixture effects of all active known and unknown chemicals in a sample, which cannot be addressed by chemical analysis alone. To date, EBM primarily been applied research context, with lower level uptake sector regulators. This is partly due to concerns regarding reliability interpretation EBM. Using evidence from peer-reviewed literature, this work aims answer frequently asked questions about The were identified through consultation industry regulators cover topics related basis using EBM, practical considerations reliability, sampling control, what do information provided give confidence stimulate application monitoring.

Language: Английский

Citations

27
Effect-Directed Analysis Combined with Nontarget Screening to Identify Unmonitored Toxic Substances in the Environment DOI Creative Commons
Seongjin Hong, Jung‐Hyun Lee, Jihyun Cha

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(48), P. 19148 - 19155

Published: Nov. 16, 2023

Effect-directed analysis (EDA) combined with nontarget screening (NTS) has established a valuable tool for the identification of unmonitored toxic substances in environmental samples. It consists three main steps: (1) highly potent fraction identification, (2) toxicant candidate selection, and (3) major identification. Here, we discuss methodology, current status, limitations, future challenges EDA NTS. This method been applied successfully to various samples, such as sediments, wastewater treatment plant effluents, biota. We present several case studies highlight key results. undergone significant technological advancements past 20 years, establishment its components: target chemical analysis, bioassays, fractionation, NTS, data processing. However, it not incorporated widely into monitoring programs. provide suggestions application NTS programs management, further research needs.

Language: Английский

Citations

23
Occurrence and potential risk of steroid hormones in selected surface water and wastewater treatment plants in western Kenya DOI Creative Commons

Isaac Cheruiyot Tanui,

Faith Jebiwot Kandie, Martin Krauß

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: 367, P. 125623 - 125623

Published: Jan. 1, 2025

Language: Английский

Citations

1
High-resolution temporal assessment of physicochemical variability and water quality in tropical semi-enclosed bays and coral reefs DOI Creative Commons
Chiara de Jong,

Iris van Os,

Guadalupe Sepúlveda-Rodríguez

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 968, P. 178810 - 178810

Published: Feb. 18, 2025

Language: Английский

Citations

1
Comprehensive Nontargeted Analysis of Drinking Water Supplies to Identify Chemicals Associated with Estrogen Receptor Agonism or Present in Regions of Elevated Breast Cancer Occurrence DOI Creative Commons
Gabrielle P. Black, B Anderson,

Luann Wong

et al.

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

To explore the hypothesis that differential exposures to estrogen active chemicals may contribute regional disparities in cancer incidence, a comprehensive targeted and nontargeted analysis was conducted over two seasons (2020) for drinking water samples from 120 households served by 8 public systems (4 with historically elevated breast incidence) 15 brands of retail water. All were analyzed using gas liquid chromatography high-resolution mass spectrometry bioassay receptor agonism. Target compounds included disinfection byproducts, per- polyfluoroalkyl substances (PFAS), trace elements, selected their possible relation cancer. Over 7500 GC LC molecular features passed all quality control filters each sampling season prioritized identification if they related measured agonism or present at higher levels areas high incidence (n = 1036). Benzothiazole-2-sulfonic acid, acetyl tributyl citrate, diphenyl sulfone among confirmed nontarget compounds. Nine polycyclic aromatic hydrocarbons ketone derivatives displayed significant negative correlations Many remained unidentified, as 84.4% 77.5% could not be annotated confidence.

Language: Английский

Citations

1