Titanium dioxide nanoparticles temporarily influence the sea urchin immunological state suppressing inflammatory-relate gene transcription and boosting antioxidant metabolic activity

Journal of Hazardous Materials, Journal Year: 2019, Volume and Issue: 384, P. 121389 - 121389

Published: Oct. 5, 2019

Titanium dioxide nanoparticles (TiO2NPs) are revolutionizing biomedicine due to their potential application as diagnostic and therapeutic agents. However, the TiO2NP immune-compatibility remains an open issue, even for ethical reasons. In this work, we investigated immunomodulatory effects of TiO2NPs in emergent proxy human non-mammalian model vitro basic translational immunology: sea urchin Paracentrotus lividus. To highlight on new insights into evolutionarily conserved intracellular signaling metabolism pathways involved immune-TiO2NP recognition/interaction applied a wide-ranging approach, including electron microscopy, biochemistry, transcriptomics metabolomics. Findings that interact with immune cells suppressing expression genes encoding proteins response apoptosis (e.g. NF-κB, FGFR2, JUN, MAPK14, FAS, VEGFR, Casp8), boosting cell antioxidant metabolic activity pentose phosphate, cysteine-methionine, glycine-serine pathways). uptake was circumscribed phagosomes/phagolysosomes, depicting harmless vesicular internalization. Our findings underlined under TiO2NP-exposure innate system is able control inflammatory signaling, excite acquire immunological tolerance, providing level understanding could be useful development Nano medicines.

Language: Английский

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Addressing Nanomaterial Immunosafety by Evaluating Innate Immunity across Living Species

Small, Journal Year: 2020, Volume and Issue: 16(21)

Published: May 1, 2020

Abstract The interaction of a living organism with external foreign agents is central issue for its survival and adaptation to the environment. Nanosafety should be considered within this perspective, it examined that how different organisms interact engineered nanomaterials (NM) by either mounting defensive response or physiologically adapting them. Herein, NM one major biological systems deputed recognition challenges, i.e., immune system, specifically addressed. main focus innate immunity, only type immunity in plants, invertebrates, lower vertebrates, coexists adaptive higher vertebrates. Because their presence majority eukaryotic organisms, responses can viewed comparative context. In cases, results reactions eliminate possible danger mechanisms do not lead damage. While some cases such may pathological consequences, other beneficial effects identified.

Language: Английский

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Transcriptional changes of Pacific oyster Crassostrea gigas reveal essential role of calcium signal pathway in response to CO2-driven acidification

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 741, P. 140177 - 140177

Published: June 12, 2020

Language: Английский

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Stem cells of aquatic invertebrates as an advanced tool for assessing ecotoxicological impacts

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 771, P. 144565 - 144565

Published: Jan. 18, 2021

Environmental stressors are assessed through methods that quantify their impacts on a wide range of metrics including species density, growth rates, reproduction, behaviour and physiology, as host-pathogen interactions immunocompetence. stress may induce additional sublethal effects, like mutations epigenetic signatures affecting offspring via germline mediated transgenerational inheritance, shaping phenotypic plasticity, increasing disease susceptibility, tissue pathologies, changes in social biological invasions. The growing diversity pollutants released into aquatic environments requires the development reliable, standardised 3R (replacement, reduction refinement animals research) compliant vitro toolbox. tools have to be line with REACH regulation 1907/2006/EC, aiming improve strategies for potential ecotoxicological risks assessment monitoring chemicals threatening human health environments. Aquatic invertebrates' adult stem cells (ASCs) numerous can pluripotent, illustrated by high regeneration ability documented many these taxa. This is further importance invertebrate taxa, ASCs able differentiate germ cells. Here we propose from key invertebrates harnessed applicable new tests ecotoxicology. As part this approach, battery modern techniques endpoints proposed tested correctly identify environmental stresses posed emerging contaminants Consequently, briefly describe current status available toxicity testing biota-based ecotoxicology highlight some associated open issues such replicability, consistency reliability outcomes, understanding assessing various organisms entire environment. Following this, benefits ASC-based better addressing questions, along obstacles possible overhaul approaches.

Language: Английский

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Interplay Between Nanoplastics and the Immune System of the Mediterranean Sea Urchin Paracentrotus lividus

Frontiers in Marine Science, Journal Year: 2021, Volume and Issue: 8

Published: March 15, 2021

The present study highlights for the first time interplay between model nanoplastics, such as carboxyl-modified polystyrene nanoparticles (PS-COOH, 60 nm) NPs and coelomocytes of sea urchin Paracentrotus lividus , a benthic grazer widely distributed in Mediterranean coastal area, upon acute vitro exposure (4 h) (5 25 μg mL –1 ). Insight into PS-COOH trafficking (uptake clearance) effects on immune cell functions (i.e., viability, lysosomal membrane stability, phagocytosis) are provided. Dynamic Light Scattering analysis reveals that PS NP suspensions CF undergo quick agglomeration, more pronounced (608.3 ± 43 compared to PS-NH 2 (329.2 5 nm). However, both still found nano-scale agglomerates after 4 h exposure, shown by polydispersity index > 0.3 associated with presence different size populations CF. observed changes ζ-potential suspension (–11.1 3 mV –12.1 respectively) confirm formation bio-corona NPs. Optical fluorescence microscopy fluorimetric analyses using fluorescently labeled (60 reveal fast uptake primarily phagocytes within 1 exposure. Upon transfer NP-free CF, significant decrease signal is observed, suggesting clearance. No effect viability PS-COOH, however stability (23.7 4.8%) phagocytic capacity (63.43 3.4%) at highest concentration tested. Similarly, reduction phagocytosis (25 ), which confirms important role surface charges triggering immunotoxicity. Overall, our results show that, although being quickly internalized, can be easily eliminated but may able trigger an response long-term scenarios. Taking account sediments along coasts sink micro- latter reach concentrations exceed toxicity-thresholds marine species.

Language: Английский

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Assessing Sunscreen Lifecycle to Minimize Environmental Risk Posed by Nanoparticulate UV-Filters – A Review for Safer-by-Design Products

Frontiers in Environmental Science, Journal Year: 2020, Volume and Issue: 8

Published: July 10, 2020

Sunscreens are of emerging concern regarding environmental effect. After leaving the skin either through bathing or washing, ingredients contained in product formulation can be released into rivers, lakes, seashores, and/or sewage treatment plants. Nanomaterials used as UV-filters particular this context they may have a negative effect on these systems. To assess risks posed, exposure and hazard nanoparticulate must considered entire lifecycle sunscreen product. This includes not only usage, but also manufacturing disposal at end life product, some nanomaterials environment each stage. developing relevant approaches that take account realistic scenarios release fate. Nanoparticulate typically consist mineral nanoparticle core (TiO2 ZnO) coated with surface layers aimed optimizing dispersion supressing any photo-sensibility. coating plays key role associated risk since it affects properties, which control both fate hazard. At present, knowledge gaps remain safety sunscreen, very few studies focused real filters formulations throughout their so far. A literature review is proposed here from design formulations, to release, fate, different compartments encountered along lifecycle. The resulting state art highlights will likely help regulators, manufacturers, consumers choose appropriate guidance. By considering development stage choice UV-filter(s) its (their) integration cosmetic involved all lifecycle, an eco-design approach achieved where toxicity reduced. Sustainability thus accounted for, during process, by making choices (in advance) minimize prevent impact sunscreen.

Language: Английский

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Occurrence of microfibres in wild specimens of adult sea urchin Paracentrotus lividus (Lamarck, 1816) from a coastal area of the central Mediterranean Sea

Marine Pollution Bulletin, Journal Year: 2022, Volume and Issue: 176, P. 113448 - 113448

Published: Feb. 24, 2022

Language: Английский

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Effect of ocean acidification on the oxygen consumption of the sea urchins Paracentrotus lividus (Lamarck, 1816) and Arbacia lixula (Linnaeus, 1758) living in CO2 natural gradients

Frontiers in Marine Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 4, 2025

Ocean acidification (OA) stands out as one of the main threats to marine ecosystems. OA leads a reduction in availability carbonate ions, which are essential for calcifiers such echinoderms. We aim understand physiological responses two sea urchin species, Paracentrotus lividus and Arbacia lixula low pH conditions determine whether their result from phenotypic plasticity or local adaptation. The study is divided into parts: response over time, measuring respiration rates individuals Mediterranean Sea exposed seven days, adaptation under changing pH, analyzing inhabiting gradient natural CO 2 vent system located La Palma Island, Spain. Over days exposure, distinct patterns were revealed, with both species demonstrating potential acclimatization. Notably, P. A. displayed unsynchronized acidosis/alkalosis cycles, suggesting different mechanisms. Additionally, environmental history seemed influence adaptive capacity, specimens fluctuating environments exhibited similar those stable heightened plasticity. Overall, our results suggest that possess capacity metabolic plasticity, may enhance resilience future scenarios but likely involve energetic costs. Moreover, systems serve refugia, facilitating long-term survival. Understanding plastic versus adaptations crucial predicting effects on distribution abundance organisms ongoing climate change.

Language: Английский

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Adaptive resilience of sea urchins against seawater acidification: A study on egg quality and offspring performance within a volcanic vents area.

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121143 - 121143

Published: Feb. 1, 2025

Language: Английский

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Characterizing biogeochemical fluctuations in a world of extremes: A synthesis for temperate intertidal habitats in the face of global change

Global Change Biology, Journal Year: 2020, Volume and Issue: 26(7), P. 3858 - 3879

Published: April 2, 2020

Coastal and intertidal habitats are at the forefront of anthropogenic influence environmental change. The species occupying these adapted to a world extremes, which may render them robust changing climate or more vulnerable if they their physiological limits. We characterized diurnal, seasonal interannual patterns flux in biogeochemistry across an gradient on temperate sandstone platform eastern Australia over 6 years (2009-2015) present synthesis our current understanding this habitat context with global used rock pools as natural mesocosms determine dynamics eco-stress experienced by resident biota. In situ measurements discrete water samples were collected night day during neap low tide events capture diurnal cycles. Calculation pHT using total alkalinity (TA) dissolved inorganic carbon (DIC) revealed that mid-intertidal exhibited greatest (pHT 7.52-8.87), single tidal cycle (1.11 units), while low-intertidal 7.82-8.30) subtidal 7.87-8.30) less variable. Temperature was also (8.0-34.5°C) event (14°C range), typical rocky shores. Mean TA DIC increased decreased day, most extreme conditions measured owing prolonged emersion periods. Temporal sampling net ecosystem calcification production highest lowest night, particularly mid-intertidal. Characterization biogeochemical fluctuations extremes demonstrates variable biota routinely experience highlight potential microhabitat-specific vulnerabilities change refugia.

Language: Английский

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