Seasonality
is
a
consistent
component
of
aquatic
ecosystems
yet
most
fish
biological
and
ecotoxicological
studies
commonly
employ
field
sampling
protocols
focused
during
the
warm
open
water
season
with
minimal
emphasis
placed
on
winter
sampling,
especially
for
north-temperate
latitude
ecosystems.
Such
strategies
limit
our
understanding
poikilotherm
biology
ecology
overwintering
seasons.
Here,
I
investigated
seasonal
changes
in
yellow
perch
(Perca
flavescens)
biology,
ecotoxicology
over
one-year
period
Lake
Manganese.
Significant
seasonality
was
observed
metrics
including
energy
densities
(kJ/g),
gonadosomatic
indices,
whole-body
lipid
contents,
carbon
stable
isotope
values
(δ13C).
Mercury
concentrations
quantified
within
single
age
class
displayed
significant
individual
variability,
Hg
fall
collected
fishes
being
higher
than
those
spring
summer
fishes.
Both
protein
mass
δ13C
were
predictors
bioaccumulation
by
Manganese
perch.
This
study
among
few
to
demonstrate
role
pollutant
bioaccumulation.
Furthermore,
these
results
need
include
establish
entire
growing
datasets
under
current
climate
change
predictions.
Limnology and Oceanography Methods,
Journal Year:
2020,
Volume and Issue:
18(8), P. 398 - 410
Published: June 9, 2020
Abstract
The
subcellular
compartmentalization
of
metals
within
aquatic
organisms
reflects
their
internal
behavior
after
metal
uptake
and
can
provide
important
information
about
potential
toxicity.
Commonly,
the
fractionation
protocol
used
to
determine
partitioning
in
consists
mechanically
homogenizing
tissue,
separating
components
into
fractions
by
differential
centrifugation
heat‐denaturation
steps,
determining
amount
associated
with
each
fraction.
However,
accurate
separation
cell
is
challenging
nature
purity
operationally
defined
are
rarely
assessed.
In
absence
this
type
validation,
however,
interpretation
results
could
be
compromised.
aim
present
study
was
adjust
a
for
liver
field‐collected
fish
test
adjusted
using
fraction‐specific
enzyme
markers.
Overall,
our
illustrate
need
optimize
procedures
when
studying
new
species
or
organ.
course
study,
categorization
some
revised
accordance
enzymatic
obtained,
order
yield
more
credible
fraction
distribution
scheme.
addition,
trial
assays
aimed
at
isolating
nuclear
materials
from
cellular
debris
were
conducted,
DNA
as
marker
material.
Tested
protocols
failed
isolate
nuclei
suggested
that
probably
trapped
disrupted
membranes.
Recommendations
on
how
improve
future
studies
freshwater
discussed.
SSRN Electronic Journal,
Journal Year:
2022,
Volume and Issue:
unknown
Published: Jan. 1, 2022
The
subcellular
partitioning
approach
provides
useful
information
on
the
location
of
metals
within
cells
and
is
often
used
organisms
with
high
level
bioaccumulation
to
establish
relationships
between
internal
concentration
toxicity
potential
metals.
Relatively
little
known
about
in
wild
fish
low
levels
comparison
those
from
contaminated
areas.
This
study
aims
examine
various
considering
their
chemical
affinity
essentiality
at
relatively
contamination
levels.
Class
A
(Y,
Sr),
class
B
(Cu,
Cd,
MeHg)
borderline
(Fe,
Mn)
metal
concentrations
were
measured
livers
fractions
yellow
perch
(n=21)
collected
Lake
Saint-Pierre,
QC,
Canada.
results
showed
that
all
metals,
apart
MeHg,
distributed
among
according
affinity.
More
than
60%
Y,
Sr,
Fe
Mn
found
metal-sensitive
fractions.
Cd
Cu
largely
metallothionein-like
proteins
peptides
(60%
67%
respectively)
whereas
MeHg
was
mainly
(85%).
In
addition,
difference
distribution
other
essential
like
Mn,
denotes
that,
although
a
determinant
affinity,
behavior
important
predict
distribution.
similarity
previous
studies
species
higher
areas
support
idea
are
cellular
environment
properties
regardless
gradient.
Seasonality
is
a
consistent
component
of
aquatic
ecosystems
yet
most
fish
biological
and
ecotoxicological
studies
commonly
employ
field
sampling
protocols
focused
during
the
warm
open
water
season
with
minimal
emphasis
placed
on
winter
sampling,
especially
for
north-temperate
latitude
ecosystems.
Such
strategies
limit
our
understanding
poikilotherm
biology
ecology
overwintering
seasons.
Here,
I
investigated
seasonal
changes
in
yellow
perch
(Perca
flavescens)
biology,
ecotoxicology
over
one-year
period
Lake
Manganese.
Significant
seasonality
was
observed
metrics
including
energy
densities
(kJ/g),
gonadosomatic
indices,
whole-body
lipid
contents,
carbon
stable
isotope
values
(δ13C).
Mercury
concentrations
quantified
within
single
age
class
displayed
significant
individual
variability,
Hg
fall
collected
fishes
being
higher
than
those
spring
summer
fishes.
Both
protein
mass
δ13C
were
predictors
bioaccumulation
by
Manganese
perch.
This
study
among
few
to
demonstrate
role
pollutant
bioaccumulation.
Furthermore,
these
results
need
include
establish
entire
growing
datasets
under
current
climate
change
predictions.
