Contrasting response of comammox Nitrospira, ammonia oxidising bacteria, and archaea to soil pH and nitrogen inputs DOI Creative Commons

C. Chisholm,

Hong J. Di, Keith C. Cameron

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 924, P. 171627 - 171627

Published: March 11, 2024

This study aimed to investigate the effect of soil pH change, and nitrogen amendment on ammonia oxidiser abundance comammox Nitrospira community composition. The experimental design used mesocosms placed in a temperature-controlled incubator for 90 days. A Templeton silt loam was as its physiochemical properties are typical region's dairy farms. results showed that clade B preferred natural (pH 6.1–6.2) with no applied nitrogen. Furthermore, synthetic urine (N700) decreased B. may have been because large amounts available N700 treatments inhibited growth Nitrospira. These suggest while present New Zealand farm soils, but their role nitrification very high environment under patch grazed pastures be limited. Further research is needed confirm this. In contrast comammox, AOB (dominated by Nitrosospira) responded positively application urine. response greatest (7.1), followed then low (4.9) soils. due difference availability. At pH, ammonia/ammonium equilibrium favours production. Calculated availability accurately predicted amoA gene abundance. Interestingly, AOA (which predominantly made up Thaumarchaeota group I.1b E) seemed prefer soils over pH. specific lineage present. did not respond

Language: Английский

Enrichment of comammox bacteria in anammox-dominated low-strength wastewater treatment system within microaerobic conditions: Cooperative effect driving enhanced nitrogen removal DOI

Huihui Cui,

Liang Zhang,

Qiong Zhang

et al.

Chemical Engineering Journal, Journal Year: 2022, Volume and Issue: 453, P. 139851 - 139851

Published: Oct. 19, 2022

Language: Английский

Citations

44
Full substitution of chemical fertilizer by organic manure decreases soil N2O emissions driven by ammonia oxidizers and gross nitrogen transformations DOI
Zewen Hei,

Yiting Peng,

Shenglei Hao

et al.

Global Change Biology, Journal Year: 2023, Volume and Issue: 29(24), P. 7117 - 7130

Published: Oct. 6, 2023

Replacing synthetic fertilizer by organic manure has been shown to reduce emissions of nitrous oxide (N2 O), but the specific roles ammonia oxidizing microorganisms and gross nitrogen (N) transformation in regulating N2 O remain unclear. Here, we examined effect completely replacing chemical with on emissions, oxidizers, N rates using a 13-year field manipulation experiment. Our results showed that reduced cumulative 16.3%-210.3% compared fertilizer. The abundance bacteria (AOB) was significantly lower during three growth stages maize. Organic also decreased AOB alpha diversity changed their community structure. However, substitution increased archaea comammox Nitrospira Interestingly, mineralization 23.2%-32.9%, autotrophic nitrification rate 10.5%-45.4%, when This study found positive correlation between abundance, emission, contribution emission supported random forest analysis. highlights key oxidizers predicting cropland O.

Language: Английский

Citations

39
Ubiquitous occurrence and functional dominance of comammox Nitrospira in full-scale wastewater treatment plants DOI
Maosheng Zheng,

Zhichao Tian,

Zimin Chai

et al.

Water Research, Journal Year: 2023, Volume and Issue: 236, P. 119931 - 119931

Published: March 29, 2023

Language: Английский

Citations

36
Co-Occurrence and Cooperation between Comammox and Anammox Bacteria in a Full-Scale Attached Growth Municipal Wastewater Treatment Process DOI Creative Commons
Katherine J. Vilardi, Irmarie Cotto, Megan Bachmann

et al.

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(12), P. 5013 - 5023

Published: March 13, 2023

Cooperation between comammox and anammox bacteria for nitrogen removal has been recently reported in laboratory-scale systems, including synthetic community constructs; however, there are no reports of full-scale municipal wastewater treatment systems with such cooperation. Here, we report intrinsic extant kinetics as well genome-resolved characterization a integrated fixed film activated sludge (IFAS) system where co-occur appear to drive loss. Intrinsic batch kinetic assays indicated that majority the aerobic ammonia oxidation was driven by (1.75 ± 0.08 mg-N/g TS-h) attached growth phase, minimal contribution ammonia-oxidizing bacteria. Interestingly, portion total inorganic (∼8%) consistently lost during these assays. Aerobic nitrite eliminated possibility denitrification cause loss, while anaerobic resulted rates consistent stoichiometry. Full-scale experiments at different dissolved oxygen (DO = 2 - 6 mg/L) setpoints persistent loss partly sensitive DO concentrations. Genome-resolved metagenomics confirmed high abundance (relative 6.53 0.34%) two Brocadia-like populations, within Ca. Nitrospira nitrosa cluster were lower (0.37 0.03%) Nitrosomonas-like oxidizers even (0.12 0.02%). Collectively, our study first time co-occurrence cooperation system.

Language: Английский

Citations

33
Key enzymes, functional genes, and metabolic pathways of the nitrogen removal-related microorganisms DOI
Chunxia Zheng, Tengxia He,

Cerong Wang

et al.

Critical Reviews in Environmental Science and Technology, Journal Year: 2024, Volume and Issue: 54(23), P. 1672 - 1691

Published: May 16, 2024

Biological nitrogen removal is a classic technique for removing from wastewater that has been studied extensively and gives high rate, energy efficiency, relatively environmentally benign. Using biological processes to treat the diverse components of requires thorough understanding microorganisms pathways involved. However, information regarding participating in remains limited. This review presents current knowledge researches into these microorganisms, including ammonia oxidizing bacteria, archaea, complete oxidation nitrite-oxidizing anaerobic ammonium denitrifying heterotrophic nitrification-aerobic denitrification bacteria. The metabolic enzymatic reactions involved cycle abovementioned are demonstrated. key enzymes, functional genes, removal-related were discussed. Additionally, advantages different oxidation, denitrifying, HN-AD described. Finally, limitations strategies unraveling presented. aim improve our principle advanced allow possible improvements treatment be identified.

Language: Английский

Citations

11
Comammox Nitrospira act as key bacteria in weakly acidic soil via potential cobalamin sharing DOI Creative Commons
Yu-Xiang Zhao,

Jiajie Hu,

Jiaqi Wang

et al.

iMeta, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

Abstract The discovery of comammox Nitrospira in low pH environments has reshaped the ammonia oxidation process acidic settings, providing a plausible explanation for higher nitrification rates observed weakly soils. However, response to varying levels and its ecological role these remains unclear. Here, survey across soils with values (ranging from 4.4 9.7) was conducted assess how perform under different conditions. Results showed that dominate soils, functioning as K‐strategy species characterized by slow growth stress tolerance. As key this environment, may promote bacterial cooperation Genomic evidence suggested cobalamin sharing is potential mechanism, uniquely encode metabolic pathway compensates imbalance where 86.8% metagenome‐assembled genomes (MAGs) cobalamin‐dependent genes. Additionally, we used DNA stable‐isotope probing (DNA‐SIP) demonstrate fluctuations reflect it responds decrease pH. confirmed became dominant oxidizers soil after We will become increasingly important global trend acidification. Overall, our work provides insights changes.

Language: Английский

Citations

1
Abundance and functional importance of complete ammonia-oxidizing bacteria (comammox) versus canonical nitrifiers in temperate forest soils DOI Creative Commons
Ernest D. Osburn, J. Barrett

Soil Biology and Biochemistry, Journal Year: 2020, Volume and Issue: 145, P. 107801 - 107801

Published: April 4, 2020

Language: Английский

Citations

70
Temperature and salinity drive comammox community composition in mangrove ecosystems across southeastern China DOI

Zongbao Liu,

Cui‐Jing Zhang, Qiaoyan Wei

et al.

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 742, P. 140456 - 140456

Published: June 25, 2020

Language: Английский

Citations

58
Iron-carbon galvanic cells strengthened anaerobic/anoxic/oxic process (Fe/C-A2O) for high-nitrogen/phosphorus and low-carbon sewage treatment DOI
Shuai Peng, Shihai Deng, Desheng Li

et al.

The Science of The Total Environment, Journal Year: 2020, Volume and Issue: 722, P. 137657 - 137657

Published: March 2, 2020

Language: Английский

Citations

52
Excellent Adsorption–Desorption of Ammonium by a Poly(acrylic acid)-Grafted Chitosan and Biochar Composite for Sustainable Agricultural Development DOI
Lixun Zhang,

Shengyin Tang,

Yuntao Guan

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2020, Volume and Issue: 8(44), P. 16451 - 16462

Published: Oct. 29, 2020

The discovery of materials as soil conditioners for the improvement nitrogen-use efficiency provides a promising strategy simultaneously addressing production and pollution problems in agriculture. Herein, adsorption–desorption performance mechanisms ammonium (NH4+–N) by poly(acrylic acid)-grafted chitosan biochar composite (PAA/CTS/BC) were studied to evaluate its potential amendment. Furthermore, mathematical models further applied explore effects PAA/CTS/BC on controlling NH4+–N loss. Results suggest that showed high capacity adsorption, with maximum value 149.25 mg·g–1 at 25 °C, considerably higher than most reported BC-based adsorbents. Fast adsorption was completed an >90% within 20 min, primarily attributed dominant electrostatic interactions carboxyl groups. Adsorbed capable being easily re-released variation liquid-phase concentration because weak binding material. These results demonstrate can be considered nitrogen slow-release medium dynamically maintain plant-available soil. Finally, simulation flow PAA/CTS/BC-amended revealed loss effectively reduced 24.18–31.77% 1.57–2.02% material dosage during 10-week rice cultivation.

Language: Английский

Citations

50