LWT,
Journal Year:
2024,
Volume and Issue:
200, P. 116159 - 116159
Published: May 1, 2024
Honey,
a
component
of
the
European
diet,
faces
contamination
challenges
that
impact
both
consumer
and
bee
health.
Glyphosate
glufosinate-based
herbicides
are
widely
used
in
agriculture
domestic
settings,
posing
potential
threats
to
humans
bees.
This
study
addresses
need
for
robust
analytical
methods
detect
glyphosate,
glufosinate,
their
metabolites
honey,
considering
complexities
matrices.
Advanced
techniques,
such
as
Liquid
Chromatography-Mass
Spectrometry
(LC-MS/MS)
Ion
Chromatography-High-Resolution
Mass
(IC-HRMS)
were
employed
method
validation
monitoring
across
97
honey
samples.
The
extraction
procedure
was
optimized,
followed
EU
Regulation
808/2021
SANTE
11312/2021
guidelines.
LC-MS/MS
IC-HRMS
demonstrated
comparability
high
sensitivity,
with
RSDr
RSDR
values
falling
within
range
3%
18%
6%
22%,
respectively,
all
analytes
considered
except
AMPA.
AMPA
showed
CV%
>
25%
at
concentration
5
10
ng/g
LC-MS/MS.
For
fall
14%
4%
range,
respectively.
quantified
12%
samples,
one
sample
exceeding
Maximum
Residue
Level
(MRL).
Glufosinate
its
not
detected
any
Scientific Reports,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: June 2, 2023
Pesticides
are
recognised
as
a
key
threat
to
pollinators,
impacting
their
health
in
many
ways.
One
route
through
which
pesticides
can
affect
pollinators
like
bumblebees
is
the
gut
microbiome,
with
knock-on
effects
on
immune
system
and
parasite
resistance.
We
tested
impacts
of
high
acute
oral
dose
glyphosate
microbiome
buff
tailed
bumblebee
(Bombus
terrestris),
glyphosate's
interaction
(Crithidia
bombi).
used
fully
crossed
design
measuring
bee
mortality,
intensity
bacterial
composition
estimated
from
relative
abundance
16S
rRNA
amplicons.
found
no
impact
either
glyphosate,
C.
bombi,
or
combination
any
metric,
including
composition.
This
result
differs
studies
honeybees,
have
consistently
an
potentially
explained
by
use
exposure,
rather
than
chronic
difference
test
species.
Since
A.
mellifera
model
species
represent
more
broadly
risk
assessment,
our
results
highlight
that
caution
needed
extrapolating
other
LWT,
Journal Year:
2024,
Volume and Issue:
200, P. 116159 - 116159
Published: May 1, 2024
Honey,
a
component
of
the
European
diet,
faces
contamination
challenges
that
impact
both
consumer
and
bee
health.
Glyphosate
glufosinate-based
herbicides
are
widely
used
in
agriculture
domestic
settings,
posing
potential
threats
to
humans
bees.
This
study
addresses
need
for
robust
analytical
methods
detect
glyphosate,
glufosinate,
their
metabolites
honey,
considering
complexities
matrices.
Advanced
techniques,
such
as
Liquid
Chromatography-Mass
Spectrometry
(LC-MS/MS)
Ion
Chromatography-High-Resolution
Mass
(IC-HRMS)
were
employed
method
validation
monitoring
across
97
honey
samples.
The
extraction
procedure
was
optimized,
followed
EU
Regulation
808/2021
SANTE
11312/2021
guidelines.
LC-MS/MS
IC-HRMS
demonstrated
comparability
high
sensitivity,
with
RSDr
RSDR
values
falling
within
range
3%
18%
6%
22%,
respectively,
all
analytes
considered
except
AMPA.
AMPA
showed
CV%
>
25%
at
concentration
5
10
ng/g
LC-MS/MS.
For
fall
14%
4%
range,
respectively.
quantified
12%
samples,
one
sample
exceeding
Maximum
Residue
Level
(MRL).
Glufosinate
its
not
detected
any