High-Throughput Phototactic Ecotoxicity Biotests with Nauplii of Artemia franciscana

Toxics, Journal Year: 2022, Volume and Issue: 10(9), P. 508 - 508

Published: Aug. 29, 2022

Analysis of sensorimotor behavioral responses to stimuli such as light can provide an enhanced relevance during rapid prioritisation chemical risk. Due technical limitations, there have been, however, only minimal studies on using invertebrate phototactic behaviors in aquatic ecotoxicity testing. In this work, we demonstrate innovative, purpose-built analytical system for a high-throughput biotest with nauplii euryhaline brine shrimp Artemia franciscana. We also, the first time, present novel and dedicated bioinformatic approach that facilitates analysis at scale great fidelity. The exhibited consistent light-seeking upon extinguishing brief programmable stimulus (5500K, 400 lux) without habituation. A proof-of-concept validation involving short-term exposure eggs (24 h) instar I larval stages (6 sub-lethal concentrations insecticides organophosphate chlorpyrifos (10 µg/L) neonicotinoid imidacloprid (50 showed perturbation seeking absence or alteration general mobility. Our preliminary data further support notion bioassays represent attractive new avenue ecotoxicology because their potential sensitivity, responsiveness, low cost.

Language: Английский

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A miniaturized electrothermal array for rapid analysis of temperature preference behaviors in ecology and ecotoxicology

Environmental Pollution, Journal Year: 2022, Volume and Issue: 314, P. 120202 - 120202

Published: Sept. 19, 2022

Language: Английский

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Sensory-Motor Perturbations in Larval Zebrafish (Danio rerio) Induced by Exposure to Low Levels of Neuroactive Micropollutants during Development

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(16), P. 8990 - 8990

Published: Aug. 12, 2022

Due to increasing numbers of anthropogenic chemicals with unknown neurotoxic properties, there is an need for a paradigm shift toward rapid and higher throughput behavioral bioassays. In this work, we demonstrate application purpose-built high multidimensional test battery on larval stages Danio rerio (zebrafish) at 5 days post fertilization (dpf). The automated comprised the established spontaneous swimming (SS), simulated predator response (SPR), photomotor (LPR) assays as well new thermotaxis (TX) assay. We applied novel system characterize environmentally relevant concentrations emerging pharmaceutical micropollutants including anticonvulsants (gabapentin: 400 ng/L; carbamazepine: 3000 ng/L), inflammatory drugs (ibuprofen: 9800 antidepressants (fluoxetine: 300 venlafaxine: 2200 ng/L). successful integration thermal preference assay into provided means reveal ibuprofen-induced perturbations behaviors upon exposure during embryogenesis. Moreover, discovered that responses in fish are also altered by yet understudied anticonvulsant gabapentin. Collectively our results utility high-throughput ecotoxicity batteries prioritizing risks associated neuroactive can perturb neurodevelopment. showcase added value bioassays preliminary screening contaminants.

Language: Английский

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Non-conventional endpoints show higher sulfoxaflor toxicity to Chironomus riparius than conventional endpoints in a multistress environment

Aquatic Toxicology, Journal Year: 2024, Volume and Issue: 275, P. 107074 - 107074

Published: Sept. 3, 2024

Evidence grows that standard toxicity testing might underestimate the environmental risk of neurotoxic insecticides. Behavioural endpoints such as locomotion and mobility have been suggested sensitive ecologically relevant additions to tested endpoints. Possible interactive effects chemicals additional stressors are typically overlooked in standardised testing. Therefore, we aimed investigate how concurrent exposure (increased temperature predation cues) a nicotinic acetylcholine receptor (nAChR)-modulating insecticide ('sulfoxaflor') impact Chironomus riparius across range conventional non-conventional We used multifactorial experimental design encompassing three stressors, sulfoxaflor (2.0-110 µg/L), (presence/absence predatory cues), elevated (20 °C 23 °C), yielding total 24 distinct treatment conditions. Additional did not change sensitivity C. sulfoxaflor. To assess potential additive effects, applied an Independent Action (IA) model predict on eight endpoints, including (growth, survival, emergence, emergence time) less (the size adults, swimming abilities exploration behaviour). For observed were either lower than expected or well-predicted by IA model. In contrast, found greater predicted cues combination with adult size, larval exploration, behaviour. However, contrast no detected stressors. Acknowledging these interactions, increasing ecological context ecotoxicological test systems may, therefore, advance analysis interpretation safe concentrations insecticides depend both organism its environment.

Language: Английский

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Behavioural Endpoints of Nile Tilapia (Oreochromis niloticus): Potential Biomarkers for Evaluating the Ecotoxicity of Contaminants of Emerging Concern and Legacy Pollutants

Royal Society of Chemistry eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 162 - 202

Published: Oct. 4, 2024

Over the last decade, a range of studies have demonstrated that various fitness-related behaviours are vulnerable to disruption induced by contaminants emerging concern (CECs) and legacy pollutants. However, behavioural responses animals when exposed pollutants/contaminants rarely included in discussions about regulation chemicals. Thus, improve our understanding opportunities challenges for ecotoxicology, this chapter presents synthesis endpoints experimental procedures used evaluate response Nile tilapia (Oreochromis niloticus – non-conventional species ecotoxicology) different CECs By focusing on methodological possibilities be evaluating behaviour Neotropical species, we shed light feasibility reasonableness using animal model as complement or alternative use traditionally toxicology.

Language: Английский

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