Comparison of Combined Dissipation Behaviors and Dietary Risk Assessments of Thiamethoxam, Bifenthrin, Dinotefuran, and Their Mixtures in Tea

Foods, Journal Year: 2024, Volume and Issue: 13(19), P. 3113 - 3113

Published: Sept. 29, 2024

In the tea-planting process, insecticides are commonly combined, potentially prolonging pre-harvest interval and heightening risk of dietary exposure. This study focused on three frequently used in tea cultivation: thiamethoxam, bifenthrin, dinotefuran, aiming to investigate their dissipation behaviors associated risks upon individual simultaneous application. The kinetics dinotefuran were successfully characterized by first-order kinetics, yielding respective half-lives 5.44, 9.81, 10.16 days. Upon joint application, thiamethoxam bifenthrin notably prolonged compared with applications, resulting final concentrations after 28 days that correspondingly elevated 1.41 1.29 times. Assessment intake revealed chronic acute quotients escalated 1.44–1.59 times following combined Although Tianmuhu white tea, as determined exposure assessment model, deemed acceptable, cumulative stemming from pesticide mixtures across various sources warrant attention. Molecular docking analyses further unveiled competitively bound glutathione S-transferase (GST) at amino acid residues, 76th GLU 25th PHE, pivotal metabolism absorption exogenous substances. Moreover, interactions between P-glycoprotein pesticides during transport likely influence post-joint research offers valuable insights data support for optimizing application strategies assessing typical cultivation.

Language: Английский

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Metabolomics as a Tool for Unraveling the Impact of Enantioselectivity in Cellular Metabolism

Critical Reviews in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 21

Published: March 4, 2025

Metabolomics is an emerging interdisciplinary field focused on the comprehensive analysis of all metabolites within biological samples, making it valuable for areas such as drug development, and environmental analysis. Many compounds, including pharmaceuticals agrochemicals that have been extensively studied by metabolomics are chiral. The intrinsic chirality targets can lead to a selective recognition enantiomers resulting in distinct pharmacokinetic, pharmacodynamic and/or toxicological profiles (enantioselectivity). Given captures instant snapshot organism's metabolic state, serves powerful tool investigate chiral compounds understand enantioselective effects. Herein, systematic compilation scientific literature was performed 48 enantioselectivity studies using were selected. These revealed increasing focus pesticides (77%), use animal models (59%), reliance LC-MS techniques (52%), predominantly untargeted approaches (83%). Enantioselective effects described most studies. This review describes significant advances this highlights unravel role stereochemistry cellular metabolism examination enantiomer-specific Furthermore, elucidates mechanism be further applied other groups compounds.

Language: Английский

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Plasmonic Gold Nanoshell-Assisted Laser Desorption/Ionization Mass Spectrometry for Small-Biomolecule Analysis and Tissue Imaging

ACS Applied Nano Materials, Journal Year: 2022, Volume and Issue: 5(7), P. 9633 - 9645

Published: June 22, 2022

Plasmonic nanoshells have been acknowledged as efficient nanomaterials for laser desorption/ionization mass spectrometry (LDI-MS) detection of a wide range small molecules, whereas their applications in imaging (MSI) are less developed. In this work, we constructed and optimized SiO2@Au with tailor-made shell structures compositions high-sensitivity LDI-MS analysis MSI applications. Owing to the synergistic effects plasmonic shells nanoscale roughness specific crevice space selective trapping molecules cations, core–shell nanoparticles exhibit superior performance vast diversity including amino acids, oligosaccharides, dyestuff drugs, peptides, nucleosides, poly(ethylene glycols). Compared organic matrices, method affords high reduction matrix interference, higher analyte coverage, lower limits ranging from fmol pmol, good repeatability relative standard deviation (RSD) below 5%. Due size homogeneous deposition nanoshells, spatial distribution various small-molecule metabolites can be visualized strawberry tissues at pixel 100 μm without artifacts. More valuably, universality SiO2@Au-assisted LDI-MSI is further demonstrated mapping lipid within whole-body zebrafish (Danio rerio), honeybees (Apis cerana), mouse brain spatially resolved manner sizes 55, 30, 50 μm, respectively. These results facilitate expansion abilities real-case Taken together, indicate that expected promising DI efficiency capabilities, especially environmental science life fields.

Language: Английский

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Ecological threat caused by malathion and its chiral metabolite in a honey bee-rape system: Stereoselective exposure risk and the mechanism revealed by proteome

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 874, P. 162585 - 162585

Published: March 3, 2023

Language: Английский

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Integrated mass spectrometry imaging and metabolomics reveals sublethal effects of indoxacarb on the red fire ant Solenopsis invicta

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(9), P. 3122 - 3132

Published: April 4, 2023

Abstract BACKGROUND Indoxacarb, representing an efficient insecticide, is normally made into a bait to spread the poison among red fire ants so that it can be widely applied in prevention and control of Solenopsis invicta . However, potential toxicity mechanism S. response indoxacarb remains explored. In this study, we integrated mass spectrometry imaging (MSI) untargeted metabolomics methods reveal disturbed metabolic expression levels spatial distribution within whole‐body tissue treated with indoxacarb. RESULTS Metabolomics results showed significantly altered level metabolites after treatment, such as carbohydrates, amino acids pyrimidine derivatives. Additionally, regulation several crucial resulting from pathway lipids visualized using label‐free MSI methods. Specifically, xylitol, aspartate, uracil were distributed throughout whole body , while sucrose‐6′‐phosphate glycerol mainly abdomen thymine was head chest Taken together, indicated closely associated disturbance key pathways, metabolism, aspartate pentose glucuronate interconversions, inhibited energy synthesis. CONCLUSION Collectively, these findings provide new perspective for understanding assessment between targeted organisms pesticides. © 2023 Society Chemical Industry.

Language: Английский

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Spatially resolved metabolomics: From metabolite mapping to function visualising

Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(11)

Published: Oct. 25, 2024

Mass spectrometry imaging (MSI)-based spatially resolved metabolomics addresses the limitations inherent in traditional liquid chromatography-tandem mass (LC-MS)-based metabolomics, particularly loss of spatial context within heterogeneous tissues. MSI not only enhances our understanding disease aetiology but also aids identification biomarkers and assessment drug toxicity therapeutic efficacy by converting invisible metabolites biological networks into visually rendered image data. In this comprehensive review, we illuminate key advancements MSI-driven over past few years. We first outline recent innovations preprocessing methodologies instrumentation that improve sensitivity comprehensiveness metabolite detection. then delve progress made functional visualization techniques, which enhance precision annotation. Ultimately, discuss significant potential applications technology translational medicine development, offering new perspectives for future research clinical translation. HIGHLIGHTS: preserves information, enhancing analysis biomarker discovery. Advances detection accuracy, expanding bioanalytical applications. Enhanced techniques refine distribution analysis. Integration with AI promises to advance accelerate development.

Language: Английский

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A facile one‐pot route to fabricate clothianidin‐loaded ZIF‐8 nanoparticles with biocompatibility and long‐term efficacy

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(7), P. 2603 - 2610

Published: March 8, 2023

Neonicotinoids are among the most essential chemical insecticides worldwide because of their high activity against many important pests and wide application. However, application is limited by toxicity to honeybees. Therefore, development a facile route fabricate efficient eco-friendly pesticide formulations great significance.In this study, clothianidin-loaded zeolitic imidazolate framework-8 (CLO@ZIF-8) nanoparticles were fabricated one-pot using zinc nitrate as Zn2+ source characterized scanning electron microscopy, transmission X-ray diffraction, thermogravimetric analysis, energy-dispersive spectrometry Fourier transform infrared spectroscopy. Based on pH response ZIF-8, 'burst release effect' was observed for CLO@ZIF-8 at 3 5 within 12 h, in contrast slow sustainable 8. improved retention ability liquid remained 70% control efficacy Nilaparvata lugens after water rinsed sprayed CLO@ZIF-8. The allowed it maintain 43% N. 10 days application, which twice clothianidin solution (SCA). Moreover, reduced acute honeybees (Apis mellifera) ≥120-fold compared with SCA.This study provides new insights into ZIF-8 neonicotinoids suggests need biocompatible formulation. © 2023 Society Chemical Industry.

Language: Английский

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Study on Absorption, Distribution, Metabolism, and Excretion Properties of Novel Insecticidal GABA Receptor Antagonist, Pyraquinil, in Diamondback Moth Combining MALDI Mass Spectrometry Imaging and High-Resolution Mass Spectrometry

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 70(20), P. 6072 - 6083

Published: May 16, 2022

A thorough understanding of absorption, distribution, metabolism, and excretion (ADME) insecticide candidates is essential in development structural optimization. Here, ADME pyraquinil, a novel insecticidal GABA receptor antagonist, Plutella xylostella larvae during the accumulation phase depuration was investigated separately using combination UHPLC-Q-Orbitrap, HPLC-MS/MS, MALDI-MSI. Five new metabolites pyraquinil were identified, metabolic pathway proposed. The oxidative metabolite (pyraquinil-sulfone) identified as main confirmed by its standard. Quantitative results showed that taken up rapidly then undergone cytochrome P450s-mediated transformation into pyraquinil-sulfone. Both fecal metabolism demonstrated to be predominant ways eliminate P. accumulation, while followed probably major depuration. MALDI-MSI revealed homogeneously distributed larvae, pyraquinil-sulfone presented continuous enrichment midgut accumulation. Conversely, located hemolymph can preferentially eliminated depuration, suggesting tissue tropism. It improves fate provides useful information for mechanism elucidation optimization pyraquinil.

Language: Английский

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Mechanism of Rotenone Toxicity against Plutella xylostella: New Perspective from a Spatial Metabolomics and Lipidomics Study

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 71(1), P. 211 - 222

Published: Dec. 20, 2022

The botanical pesticide rotenone can effectively control target pest Plutella xylostella, yet insights into in situ metabolic regulation of P. xylostella toward remain limited. Herein, we demonstrated expression levels and spatial distribution rotenone-treated using metabolomics lipidomics. Specifically, significantly affected purine amino acid metabolisms, indicating that adenosine monophosphate inosine were distributed the whole body with elevated levels, while guanosine 5'-monophosphate tryptophan downregulated. Spatial lipidomics results indicated may destroy glycerophospholipids cell membranes inhibit fatty biosynthesis, consume diacylglycerol to enhance fat oxidation. These findings revealed high toxicity be ascribed negative effects on energy production synthesis damage membranes, providing guidelines for mechanism pests rational development candidates.

Language: Английский

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An Exploratory Study of the Metabolite Profiling from Pesticides Exposed Workers

Metabolites, Journal Year: 2023, Volume and Issue: 13(5), P. 596 - 596

Published: April 27, 2023

Pesticides constitute a category of chemical products intended specifically for the control and mitigation pests. With their constant increase in use, risk to human health environment has increased proportionally due occupational environmental exposure these compounds. The use chemicals is associated with several toxic effects related acute chronic toxicity, such as infertility, hormonal disorders cancer. present work aimed study metabolic profile individuals occupationally exposed pesticides, using metabolomics tool identify potential new biomarkers. Metabolomics analysis was carried out on plasma urine samples from non-exposed occupationally, liquid chromatography coupled mass spectrometry (UPLC-MS). Non-targeted analysis, principal component (PCA), partial least squares discriminant (PLS-DA) or orthogonal (OPLS-DA), demonstrated good separation identified 21 discriminating metabolites 17 urine. ROC curve indicated compounds greatest Comprehensive pathways influenced by pesticides revealed alterations, mainly lipid amino acid metabolism. This indicates that provides important information about complex biological responses.

Language: Английский

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