The Science of The Total Environment,
Journal Year:
2022,
Volume and Issue:
864, P. 161023 - 161023
Published: Dec. 18, 2022
The
early
warning
and
tracking
of
COVID-19
prevalence
in
the
community
provided
by
wastewater
surveillance
has
highlighted
its
potential
for
much
broader
viral
disease
surveillance.
In
this
proof-of-concept
study,
46
samples
from
four
treatment
plants
(WWTPs)
Queensland,
Australia,
were
analyzed
presence
abundance
13
respiratory
viruses,
results
compared
with
reported
clinical
cases.
viruses
concentrated
using
adsorption-extraction
(AE)
method,
extracted
nucleic
acids
qPCR
RT-qPCR.
Among
tested,
bocavirus
(BoV),
parechovirus
(PeV),
rhinovirus
A
(RhV
A)
B
B)
detected
all
samples.
All
tested
except
influenza
virus
(IBV)
sample
at
least
one
WWTP.
BoV
was
greatest
concentration
(4.96-7.22
log
Water Research,
Journal Year:
2022,
Volume and Issue:
213, P. 118132 - 118132
Published: Feb. 3, 2022
Effective
wastewater
surveillance
of
SARS-CoV-2
RNA
requires
the
rigorous
characterization
limit
detection
resulting
from
entire
sampling
process
-
(PLOD).
Yet
to
date,
no
studies
have
gone
beyond
quantifying
assay
(ALOD)
for
RT-qPCR
or
RT-dPCR
assays.
While
ALOD
is
lowest
number
gene
copies
(GC)
associated
with
a
95%
probability
in
single
PCR
reaction,
PLOD
represents
sensitivity
method
after
considering
efficiency
all
processing
steps
(e.g.,
sample
handling,
concentration,
nucleic
acid
extraction,
and
assays)
determine
GC
matrix
specific
detection.
The
primary
objective
this
study
was
estimate
combination
concentration
extraction
six
assays:
five
assays
(US
CDC
N1
N2,
China
N
ORF1ab
(CCDC
CCDC
ORF1ab),
E_Sarbeco
RT-qPCR,
one
RT-dPCR)
using
two
models
(exponential
survival
cumulative
Gaussian).
An
adsorption
(AE)
(i.e.,
virus
on
membrane
membrane)
used
concentrate
gamma-irradiated
seeded
into
36
samples.
Overall,
US
had
ALODs
(<
10
GC/reaction)
PLODs
(<3,954
GC/50
mL;
detection)
regardless
seeding
level
model
used.
Nevertheless,
consistent
amplification
rates
decreased
when
levels
were
<
2.32
×
103
mL
even
Consequently,
concentrations
are
expected
be
low,
it
may
necessary
improve
positive
by
analyzing
additional
field
RT-PCR
replicates.
To
best
our
knowledge,
first
assess
provides
important
insights
analytical
limitations
trace
wastewater.
PLoS ONE,
Journal Year:
2023,
Volume and Issue:
18(4), P. e0284370 - e0284370
Published: April 12, 2023
Wastewater-based
epidemiology
(WBE)
has
become
a
valuable
tool
for
monitoring
SARS-CoV-2
infection
trends
throughout
the
COVID-19
pandemic.
Population
biomarkers
that
measure
relative
human
fecal
contribution
to
normalize
wastewater
concentrations
are
needed
improved
analysis
and
interpretation
of
community
trends.
The
Centers
Disease
Control
Prevention
National
Wastewater
Surveillance
System
(CDC
NWSS)
recommends
using
flow
rate
or
indicators
as
population
normalization
factors.
However,
there
is
no
consensus
on
which
factor
performs
best.
In
this
study,
we
provided
first
multistate
assessment
effects
(crAssphage,
F+
Coliphage,
PMMoV)
correlation
cases
CDC
NWSS
dataset
182
communities
across
six
U.S.
states.
Flow
normalized
produced
strongest
with
cases.
from
three
were
significantly
lower
than
rate.
Additionally,
reverse
transcription
droplet
digital
polymerase
chain
reaction
(RT-ddPCR)
values
over
samples
analyzed
real-time
quantitative
(rRT-qPCR).
Our
shows
utilizing
RT-ddPCR
generate
between
ACS ES&T Water,
Journal Year:
2022,
Volume and Issue:
2(12), P. 2460 - 2470
Published: Oct. 10, 2022
In
the
context
of
COVID-19
pandemic,
wastewater-based
epidemiology
(WBE)
emerged
as
a
useful
tool
to
account
for
prevalence
SARS-CoV-2
infections
on
population
scale.
this
study,
we
analyzed
wastewater
samples
from
three
large
(>300,000
people
served)
and
four
small
(<25,000
communities
throughout
southern
Germany
August
December
2021,
capturing
fourth
infection
wave
in
dominated
by
Delta
variant
(B.1.617.2).
As
dilution
can
skew
biomarker
concentrations
wastewater,
normalization
parameters
improve
relationship
between
data
clinical
data.
investigated
suitability
performance
various
parameters.
Influent
flow
showed
strong
relationships
precipitation
data;
accordingly,
flow-normalization
reacted
distinctly
events.
Normalization
surrogate
viruses
CrAssphage
pepper
mild
mottle
virus
varying
different
sampling
sites.
The
best
was
achieved
with
mixed
fecal
indicator
calculated
both
viruses.
Analyzing
temporal
spatial
variation
proved
be
explain
performance.
Overall,
our
findings
indicate
that
viruses,
flow,
hydro-chemical
is
site-specific.
We
recommend
testing
individually
specific
sewage
systems.
