Leveraging Wastewater: Validating the National-Scale SARS-CoV-2 Surveillance System in Cyprus for Elevated Public Health Surveillance and Enhanced Epidemiological Insight DOI
Popi Karaolia, Annalisa Quattrocchi, Jan Richter

et al.

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(3), P. 112953 - 112953

Published: May 6, 2024

Language: Английский

Correlation between SARS-CoV-2 RNA concentration in wastewater and COVID-19 cases in community: A systematic review and meta-analysis DOI Creative Commons
Xuan Li, Shuxin Zhang, Samendra P. Sherchan

et al.

Journal of Hazardous Materials, Journal Year: 2022, Volume and Issue: 441, P. 129848 - 129848

Published: Aug. 28, 2022

Language: Английский

Citations

90

Artificial neural network-based estimation of COVID-19 case numbers and effective reproduction rate using wastewater-based epidemiology DOI
Guangming Jiang, Jiangping Wu, Jennifer Weidhaas

et al.

Water Research, Journal Year: 2022, Volume and Issue: 218, P. 118451 - 118451

Published: April 13, 2022

Language: Английский

Citations

78

Occurrence of multiple respiratory viruses in wastewater in Queensland, Australia: Potential for community disease surveillance DOI Creative Commons
Warish Ahmed, Aaron Bivins,

Mikayla Stephens

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 864, P. 161023 - 161023

Published: Dec. 18, 2022

The early warning and tracking of COVID-19 prevalence in the community provided by wastewater surveillance has highlighted its potential for much broader viral disease surveillance. In this proof-of-concept study, 46 samples from four treatment plants (WWTPs) Queensland, Australia, were analyzed presence abundance 13 respiratory viruses, results compared with reported clinical cases. viruses concentrated using adsorption-extraction (AE) method, extracted nucleic acids qPCR RT-qPCR. Among tested, bocavirus (BoV), parechovirus (PeV), rhinovirus A (RhV A) B B) detected all samples. All tested except influenza virus (IBV) sample at least one WWTP. BoV was greatest concentration (4.96-7.22 log

Language: Английский

Citations

75

Evaluation of process limit of detection and quantification variation of SARS-CoV-2 RT-qPCR and RT-dPCR assays for wastewater surveillance DOI Creative Commons
Warish Ahmed, Aaron Bivins, Suzanne Metcalfe

et al.

Water Research, Journal Year: 2022, Volume and Issue: 213, P. 118132 - 118132

Published: Feb. 3, 2022

Effective wastewater surveillance of SARS-CoV-2 RNA requires the rigorous characterization limit detection resulting from entire sampling process - (PLOD). Yet to date, no studies have gone beyond quantifying assay (ALOD) for RT-qPCR or RT-dPCR assays. While ALOD is lowest number gene copies (GC) associated with a 95% probability in single PCR reaction, PLOD represents sensitivity method after considering efficiency all processing steps (e.g., sample handling, concentration, nucleic acid extraction, and assays) determine GC matrix specific detection. The primary objective this study was estimate combination concentration extraction six assays: five assays (US CDC N1 N2, China N ORF1ab (CCDC CCDC ORF1ab), E_Sarbeco RT-qPCR, one RT-dPCR) using two models (exponential survival cumulative Gaussian). An adsorption (AE) (i.e., virus on membrane membrane) used concentrate gamma-irradiated seeded into 36 samples. Overall, US had ALODs (< 10 GC/reaction) PLODs (<3,954 GC/50 mL; detection) regardless seeding level model used. Nevertheless, consistent amplification rates decreased when levels were < 2.32 × 103 mL even Consequently, concentrations are expected be low, it may necessary improve positive by analyzing additional field RT-PCR replicates. To best our knowledge, first assess provides important insights analytical limitations trace wastewater.

Language: Английский

Citations

65

A multistate assessment of population normalization factors for wastewater-based epidemiology of COVID-19 DOI Creative Commons
Andrew Rainey,

Song Liang,

Joseph H. Bisesi

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(4), P. e0284370 - e0284370

Published: April 12, 2023

Wastewater-based epidemiology (WBE) has become a valuable tool for monitoring SARS-CoV-2 infection trends throughout the COVID-19 pandemic. Population biomarkers that measure relative human fecal contribution to normalize wastewater concentrations are needed improved analysis and interpretation of community trends. The Centers Disease Control Prevention National Wastewater Surveillance System (CDC NWSS) recommends using flow rate or indicators as population normalization factors. However, there is no consensus on which factor performs best. In this study, we provided first multistate assessment effects (crAssphage, F+ Coliphage, PMMoV) correlation cases CDC NWSS dataset 182 communities across six U.S. states. Flow normalized produced strongest with cases. from three were significantly lower than rate. Additionally, reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) values over samples analyzed real-time quantitative (rRT-qPCR). Our shows utilizing RT-ddPCR generate between

Language: Английский

Citations

29

COVID-19 monitoring with sparse sampling of sewered and non-sewered wastewater in urban and rural communities DOI Creative Commons
Dhammika Leshan Wannigama, Mohan Amarasiri, Parichart Hongsing

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(7), P. 107019 - 107019

Published: June 9, 2023

Language: Английский

Citations

27

Current state and future perspectives on de facto population markers for normalization in wastewater-based epidemiology: A systematic literature review DOI
Tim Boogaerts, Natan Van Wichelen, Maarten Quireyns

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 935, P. 173223 - 173223

Published: May 16, 2024

Language: Английский

Citations

12

Wastewater-Based Epidemiology for SARS-CoV-2 Biomarkers: Evaluation of Normalization Methods in Small and Large Communities in Southern Germany DOI

Alexander Mitranescu,

Anna Uchaikina,

Anna‐Sonia Kau

et al.

ACS ES&T Water, Journal Year: 2022, Volume and Issue: 2(12), P. 2460 - 2470

Published: Oct. 10, 2022

In the context of COVID-19 pandemic, wastewater-based epidemiology (WBE) emerged as a useful tool to account for prevalence SARS-CoV-2 infections on population scale. this study, we analyzed wastewater samples from three large (>300,000 people served) and four small (<25,000 communities throughout southern Germany August December 2021, capturing fourth infection wave in dominated by Delta variant (B.1.617.2). As dilution can skew biomarker concentrations wastewater, normalization parameters improve relationship between data clinical data. investigated suitability performance various parameters. Influent flow showed strong relationships precipitation data; accordingly, flow-normalization reacted distinctly events. Normalization surrogate viruses CrAssphage pepper mild mottle virus varying different sampling sites. The best was achieved with mixed fecal indicator calculated both viruses. Analyzing temporal spatial variation proved be explain performance. Overall, our findings indicate that viruses, flow, hydro-chemical is site-specific. We recommend testing individually specific sewage systems.

Language: Английский

Citations

28

Wastewater-based epidemiology for COVID-19 surveillance and beyond: A survey DOI Creative Commons
Chen Chen,

Yunfan Wang,

Gursharn Kaur

et al.

Epidemics, Journal Year: 2024, Volume and Issue: 49, P. 100793 - 100793

Published: Sept. 26, 2024

Language: Английский

Citations

6

Application of neighborhood-scale wastewater-based epidemiology in low COVID-19 incidence situations DOI Open Access
Chamteut Oh,

Aijia Zhou,

Kate O’Brien

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 852, P. 158448 - 158448

Published: Sept. 2, 2022

Language: Английский

Citations

21