The Diversity, Resistance Profiles and Plasmid Content of Klebsiella spp. Recovered from Dairy Farms Located around Three Cities in Pakistan

Antibiotics, Journal Year: 2023, Volume and Issue: 12(3), P. 539 - 539

Published: March 8, 2023

The rise of antimicrobial resistance (AMR) in bacterial pathogens such as Klebsiella pneumoniae (Kp) is a pressing public health and economic concern. 'One-Health' framework recognizes that effective management AMR requires surveillance agricultural well clinical settings, particularly low-resource regions Pakistan. Here, we use whole-genome sequencing to characterise 49 isolates Klebisella spp. (including 43 Kp) 2 presumptive Providencia rettgeri recovered from dairy farms located near 3 cities Pakistan-Quetta (n = 29), Faisalabad 19), Sargodha 3). Kp corresponded 38 sequence types (STs), 35 these STs were only observed once. This high diversity indicates frequent admixture limited clonal spread on local scales. Of the isolates, 41 (84%) did not contain any clinically relevant genes (ARGs), detect ARGs predicted encode carbapenems or colistin. However, four lineages contained multiple ARGs: ST11 2), ST1391-1LV 1), ST995 1) ST985 1). 11, 1391-1LV 995 shared core set five ARGs, including blaCTX-M-15, harboured different plasmids. carried 16 genes, blaCTX-M-55. two P. also ARGs. Finally, most common plasmids which harbour our dataset non-randomly distributed between regions, suggesting expansion occurs independently host lineage. Evidence regarding how contribute emergence Pakistan valuable for authorities organizations responsible health, agriculture environment, industrial development.

Language: Английский

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New Delhi metallo-β-lactamase-1-producing Citrobacter portucalensis belonging to the novel ST264 causing fatal sepsis in a vulnerable migratory sea turtle

One Health, Journal Year: 2023, Volume and Issue: 17, P. 100590 - 100590

Published: June 23, 2023

Olive ridley (Lepidochelys olivacea) turtles migrate across tropical regions of the Atlantic, Pacific, and Indian Oceans. Worryingly, olive populations have been declining substantially is now considered a threatened species. In this regard, habitat degradation, anthropogenic pollution, infectious diseases most notorious threats for We isolated metallo-β-lactamase (NDM-1)-producing Citrobacter portucalensis from blood sample an infected migratory turtle found stranded sick in coast Brazil. Genomic analysis C. confirmed novel sequence type (ST), named ST264, wide resistome to broad-spectrum antibiotics. The production NDM-1 by strain contributed treatment failure death animal. Phylogenomic relationship with environmental human strains African, European Asian countries that critical priority clones are spreading beyond hospital settings, representing emerging ecological threat marine ecosystems.

Language: Английский

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WHO Critical Priority Escherichia coli in Latin America: A One Health Challenge for a Post-Pandemic World

Springer eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 1 - 32

Published: Jan. 1, 2023

Language: Английский

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Environmental antimicrobial resistance gene detection from wild bird habitats using two methods: A commercially available culture-independent qPCR assay and culture of indicator bacteria followed by whole-genome sequencing

Journal of Global Antimicrobial Resistance, Journal Year: 2023, Volume and Issue: 33, P. 186 - 193

Published: March 25, 2023

A variety of methods have been developed to detect antimicrobial resistance (AMR) in different environments better understand the evolution and dissemination this public health threat. Comparisons results generated using AMR detection methods, such as quantitative PCR (qPCR) whole-genome sequencing (WGS), are often imperfect, few studies analysed samples parallel evaluate differences. In study, we compared bacterial culture WGS a culture-independent commercially available qPCR assay concordance between utility each answering research questions regarding presence epidemiology wild bird habitats.We first assessed gene 45 isolates from which had existing data. We then 52 faecal 9 spatiotemporally collected water phenotypically resistant indicator isolates.Overall was strong isolates, although differed among antibiotic classes. Analysis revealed that more were determined be positive for via than did not genes two found.Both followed by may effective approaches characterising harboured birds, data streams produced these tools advantages disadvantages should considered given application sample matrix.

Language: Английский

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Metallo-β-lactamases

Elsevier eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 157 - 184

Published: Sept. 8, 2023

Language: Английский

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