The Science of The Total Environment,
Journal Year:
2025,
Volume and Issue:
978, P. 179362 - 179362
Published: April 19, 2025
In
recent
years,
the
analysis
of
viruses
in
wastewater
has
become
a
recognized
method
to
monitor
circulation
pathogenic
within
populations.
This
non-invasive
and
integrated
approach
is
all
more
strategic
as
many
viral
pathogens
can
be
released
sewage.
There
currently
no
standard
for
virological
wastewater,
various
protocols
have
been
developed
concentrate
detect
this
matrix.
study
aims
develop
high-throughput
qPCR
detection
system
describe
occurrence
30
human
enteric
respiratory
influent
effluent
samples
collected
Paris
area
during
two
periods
2023.
The
influence
concentration
(ultracentrifugation
or
aluminium
hydroxide
adsorption-precipitation)
recovery
from
was
also
investigated.
ultracentrifugation-based
allowed
higher
(4.95-100
%
versus
0.77-33
precipitation-based
method),
especially
waters.
PCR
array
revealed
presence
viruses,
with
marked
seasonal
variations
mainly
viruses.
particularly
suitable
rapid,
sensitive,
specific
multiple
wastewater.
Scientific Reports,
Journal Year:
2022,
Volume and Issue:
12(1)
Published: Sept. 22, 2022
Recurrent
influenza
epidemics
and
pandemic
potential
are
significant
risks
to
global
health.
Public
health
authorities
use
clinical
surveillance
locate
monitor
influenza-like
cases
outbreaks
mitigate
hospitalizations
deaths.
Currently,
integration
of
is
the
only
reliable
method
for
reporting
types
subtypes
warn
emergent
strains.
The
utility
wastewater
(WWS)
during
COVID-19
as
a
less
resource
intensive
replacement
or
complement
has
been
predicated
on
analyzing
viral
fragments
in
wastewater.
We
show
here
that
virus
targets
stable
partitions
favorably
solids
fraction.
By
quantifying,
typing,
subtyping
municipal
primary
sludge
community
outbreak,
we
forecasted
citywide
flu
outbreak
with
17-day
lead
time
provided
population-level
near
real-time
feasibility
WWS
at
neighbourhood
levels
real
using
minimal
resources
infrastructure.
American Journal of Epidemiology,
Journal Year:
2022,
Volume and Issue:
192(2), P. 305 - 322
Published: Oct. 13, 2022
Abstract
Wastewater
surveillance
for
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
has
been
shown
to
be
a
valuable
source
of
information
regarding
SARS-CoV-2
transmission
and
disease
2019
(COVID-19)
cases.
Although
the
method
used
several
decades
track
other
infectious
diseases,
there
not
comprehensive
review
outlining
all
pathogens
that
have
surveilled
through
wastewater.
Herein
we
identify
diseases
previously
studied
via
wastewater
prior
COVID-19
pandemic.
Infectious
were
identified
in
100
studies
across
38
countries,
as
themes
how
measures
linked.
Twenty-five
separate
pathogen
families
included
studies,
with
majority
examining
from
family
Picornaviridae,
including
polio
nonpolio
enteroviruses.
Most
did
link
what
was
found
transmission.
Among
those
did,
value
reported
varied
by
study.
should
considered
potential
public
health
tool
many
diseases.
can
improved
incorporating
at
population-level
incidence
hospitalizations.
Environment International,
Journal Year:
2024,
Volume and Issue:
183, P. 108438 - 108438
Published: Jan. 1, 2024
The
increased
frequency
of
human
infectious
disease
outbreaks
caused
by
RNA
viruses
worldwide
in
recent
years
calls
for
enhanced
public
health
surveillance
better
future
preparedness.
Wastewater-based
epidemiology
(WBE)
is
emerging
as
a
valuable
epidemiological
tool
providing
timely
population-wide
prevention
and
response
complementary
to
the
current
clinical
system.
Here,
we
compared
analytical
performance
practical
applications
between
predominant
molecular
detection
methods
RT-qPCR
RT-ddPCR
on
SARS-CoV-2
wastewater
surveillance.
When
pure
viral
was
tested,
exhibited
superior
quantification
accuracy
at
higher
concentration
levels
achieved
more
sensitive
with
reduced
variation
low
levels.
Furthermore,
consistently
demonstrated
robust
accurate
measurement
either
background
matrix
or
presence
mismatches
target
regions
consensus
assay.
Additionally,
detecting
mock
variant
samples,
found
that
outperformed
virus
genotyping
targeting
specific
loci
signature
mutations
allele-specific
(AS)
assays,
especially
allele
frequencies
concentrations,
which
possibility
low-prevalence
population.
Our
study
provides
insights
method
selection
WBE
outbreaks.
Water Research,
Journal Year:
2024,
Volume and Issue:
259, P. 121879 - 121879
Published: June 3, 2024
Wastewater-based
epidemiology
(WBE)
has
been
demonstrably
successful
as
a
relatively
unbiased
tool
for
monitoring
levels
of
SARS-CoV-2
virus
circulating
in
communities
during
the
COVID-19
pandemic.
Accumulated
biobanks
wastewater
samples
allow
retrospective
exploration
spatial
and
temporal
trends
public
health
indicators
such
chemicals,
viruses,
antimicrobial
resistance
genes,
possible
emergence
novel
human
or
zoonotic
pathogens.
We
investigated
resilience
to
time,
temperature,
freeze-thaw
cycles,
plus
optimal
storage
conditions
maintain
stability
genetic
material
(RNA/DNA)
viral
+ssRNA
(Envelope
-
E,
Nucleocapsid
N
Spike
protein
S
genes
SARS-CoV-2),
dsRNA
(Phi6
phage)
circular
dsDNA
(crAssphage)
wastewater.
Samples
consisted
(i)
processed
extracted
samples,
(ii)
distilled
water
(iii)
raw,
unprocessed
samples.
were
stored
at
-80
°C,
-20
4
20
°C
10
days,
going
through
up
cycles
(once
per
day).
Sample
was
measured
using
reverse
transcription
quantitative
PCR,
automated
electrophoresis,
short-read
whole
genome
sequencing.
Exploring
different
areas
demonstrated
that
gene
showed
greater
sensitivity
than
E
genes.
Investigating
surrogate
normalisation
viruses
Phi6
remains
stable
comparison
laboratory
setting
crAssphage
resilient
temperature
variation.
Recovery
raw
significantly
when
which
supported
by
sequencing
data
all
both
time
negatively
impacted
metrics.
Historical
extracts
re-quantified
12,
14
16
months
after
original
quantification
no
major
changes.
This
study
highlights
importance
fast
processing
extraction
following
are
robust
range
temperatures.
Current Opinion in Environmental Science & Health,
Journal Year:
2022,
Volume and Issue:
31, P. 100396 - 100396
Published: Oct. 6, 2022
Wastewater-Based
Epidemiological
Monitoring
(WBEM)
is
an
efficient
surveillance
tool
during
the
COVID-19
pandemic
as
it
meets
all
requirements
of
a
complete
monitoring
system
including
early
warning,
tracking
current
trend,
prevalence
disease,
detection
genetic
diversity
well
asthe
up-surging
SARS-CoV-2
new
variants
with
mutations
from
wastewater
samples.
Subsequently,
Clinical
Diagnostic
Test
widely
acknowledged
global
gold
standard
method
for
disease
monitoring,
despite
several
drawbacks
such
high
diagnosis
cost,
reporting
bias,
and
difficulty
asymptomatic
patients
(silent
spreaders
infection
who
manifest
nosymptoms
disease).
In
this
reviewand
opinion-based
study,
we
first
propose
combined
approach)
detecting
in
communities
using
clinical
sample
testing,
which
may
be
feasible
effective
emerging
public
health
long-term
nationwide
system.
The
viral
concentrations
samples
can
used
indicatorsto
monitor
ongoing
trends,
predict
carriers,
detect
hotspot
areas,
while
sampleshelp
mostlysymptomaticindividuals
isolating
positive
cases
validate
WBEM
protocol
mass
vaccination
booster
doses
COVID-19.
