Observations of Respiratory Syncytial Virus (RSV) Nucleic Acids in Wastewater Solids Across the United States in the 2022–2023 Season: Relationships with RSV Infection Positivity and Hospitalization Rates

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 4(4), P. 1657 - 1667

Published: Feb. 29, 2024

Respiratory syncytial virus (RSV) is a leading cause of respiratory illness and hospitalization, but clinical surveillance detects only minority cases. Wastewater could determine the onset extent RSV circulation in absence sensitive case detection, to date, studies wastewater are few. We measured RNA concentrations solids from 176 sites during 2022–2023 season compared those publicly available infection positivity hospitalization rates. Concentrations ranged undetectable 107 copies per gram. concentration aggregated at state national levels correlated with was determined using both rates independent algorithms for 14 states where data were start season. In 4 states, identified same week; 3 preceded onset, 7 occurred after onset. generally peaked week as peaked. Differences peaks versus may reflect inherent differences approaches.

Language: Английский

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From capture to detection: A critical review of passive sampling techniques for pathogen surveillance in water and wastewater

Water Research, Journal Year: 2024, Volume and Issue: 261, P. 122024 - 122024

Published: June 30, 2024

Water quality, critical for human survival and well-being, necessitates rigorous control to mitigate contamination risks, particularly from pathogens amid expanding urbanization. Consequently, the necessity maintain microbiological safety of water supplies demands effective surveillance strategies, reliant on collection representative samples precise measurement contaminants. This review critically examines advancements passive sampling techniques monitoring in various systems, including wastewater, freshwater, seawater. We explore evolution conventional materials innovative adsorbents pathogen capture shift culture-based molecular detection methods, underscoring adaptation this field global health challenges. The comparison highlights sampling's efficacy over like grab its potential overcome existing challenges through use such as granular activated carbon, thermoplastics, polymer membranes. By evaluating literature, work identifies standardization gaps proposes future research directions augment efficiency, specificity, utility environmental public surveillance.

Language: Английский

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Determination of oxyphenisatine and its total ester derivatives content in fermented green plum by ultra performance liquid chromatography-tandem mass spectrometry

Food Additives & Contaminants Part A, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: Jan. 9, 2025

Illegal additives such as oxyphenisatine and its esters are prevalent in the slimming food industry, necessitating a robust analytical method for their detection. This study presents novel UPLC-MS/MS rapid accurate quantification of total levels fermented green plum, following hydrolysis esters. An efficient ultrasonic extraction with methanol 0.1 mol/L NaOH mixture (5:5, v/v) was optimised to hydrolyse within 18 min. Chromatographic separation conducted on C18 column (Waters Acquity UPLC BEH, 2.1 × 100 mm, 1.7 μm) mobile phase 5 mmol/L ammonium acetate acetonitrile under gradient elution at flow rate 0.3 mL/min. The demonstrated linearity (r2 > 0.999) over 0.1–500 µg/L, LOD 10 µg/kg LOQ 30 µg/kg. Quantitative analysis employed positive ion multi-response monitoring external standardisation, achieving recoveries 92.4–97.0% RSDs 2.9–4.1%. Application ten real samples gave 90% detection rate, measured values closely aligning theoretical predictions (−11.3 13.2% relative difference) content ranging from 159 452 mg/kg. provides reliable tool presence derivatives context safety.

Language: Английский

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Bacterial Wastewater-Based Epidemiology Using Surface-Enhanced Raman Spectroscopy and Machine Learning

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Although wastewater-based epidemiology has been used extensively for the surveillance of viral diseases, it not to a similar extent bacterial diseases. This is in part owing difficulties distinguishing pathogenic from nonpathogenic bacteria using PCR methods. Here, we show that surface-enhanced Raman spectroscopy (SERS) can be scalable, label-free method detection wastewater. We enhance signal wastewater plasmonic gold nanorods (AuNRs) electrostatically bind surface and confirm this binding cryoelectron microscopy. spike four clinically relevant species AuNRs into filtered wastewater, varying AuNR concentration maximize signal. then collect 540 spectra each at 109 cells/mL train machine learning model identify them with more than 87% accuracy. also demonstrate an environmentally realistic limit 104 cells/mL. These results are key step toward SERS platform WBE.

Language: Английский

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A sample-to-answer digital microfluidic multiplexed PCR system for syndromic pathogen detection in respiratory tract infection

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

We report a high-performance, fully automated digital microfluidic PCR system for point-of-care testing of multiple pathogens in just 80 min.

Language: Английский

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Analysis Insights to Support the Use of Wastewater and Environmental Surveillance Data for Infectious Diseases and Pandemic Preparedness

Epidemics, Journal Year: 2025, Volume and Issue: unknown, P. 100825 - 100825

Published: March 1, 2025

Wastewater-based epidemiology is the detection of pathogens from sewage systems and interpretation these data to improve public health. Its use has increased in scope since 2020, when it was demonstrated that SARS-CoV-2 RNA could be successfully extracted wastewater affected populations. In this Perspective we provide an overview recent advances pathogen within wastewater, propose a framework for identifying utility sampling suggest areas where analytics require development. Ensuring both collection analysis are tailored towards key questions at different stages epidemic will inference made. For analyses useful methods determine absence infection, early reliably estimate trajectories prevalence, detect novel variants without reliance on consensus sequences. This research area included many innovations have improved collected optimistic innovation continue future.

Language: Английский

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Advancing multiplex diagnostics: A novel framework for simultaneous detection of diverse pathogens using respiratory RNA viruses as a model system

Journal of Immunological Methods, Journal Year: 2025, Volume and Issue: unknown, P. 113857 - 113857

Published: April 1, 2025

Language: Английский

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Spatial and temporal variation in respiratory syncytial virus (RSV) subtype RNA in wastewater and relation to clinical specimens

mSphere, Journal Year: 2024, Volume and Issue: 9(7)

Published: June 27, 2024

Respiratory syncytial virus (RSV) causes a large burden of respiratory illness globally. It has two subtypes, RSV A and B, but little is known regarding the predominance these subtypes during different seasons their impact on morbidity mortality. Using molecular methods, we quantified B RNA in wastewater solids across multiple metropolitan areas to gain insight into subtypes. We determined predominant subtype for each group using proportion total (RSV + B) sample (

Language: Английский

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Online dashboards for SARS-CoV-2 wastewater-based epidemiology

Future Microbiology, Journal Year: 2024, Volume and Issue: 19(9), P. 761 - 769

Published: May 23, 2024

Aim: Wastewater-based epidemiology (WBE) is increasingly used to monitor pandemics. In this manuscript, we review methods and limitations of WBE, as well their online dashboards. Materials & methods: Online dashboards were retrieved using PubMed search engines, annotated for timeliness, availability English version, details on SARS-CoV-2 sublineages, normalization by population PPMoV load, case/hospitalization count charts raw data export. Results: We 51 web portals, half them from Europe. Africa represented South only, only seven portals are available Asia. Conclusion: WBS provides near-real-time cost-effective monitoring analytes across space time in populations. However, tremendous heterogeneity still persists the WBE literature.

Language: Английский

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Simultaneous detection of influenza A, B and respiratory syncytial virus in wastewater samples by one-step multiplex RT-ddPCR assay

Human Genomics, Journal Year: 2024, Volume and Issue: 18(1)

Published: May 20, 2024

Abstract Background After the occurrence of COVID-19 pandemic, detection other disseminated respiratory viruses using highly sensitive molecular methods was declared essential for monitoring spread health-threatening in communities. The development multiplex assays are simultaneous such even at low concentrations. In present study, a and specific one-step droplet digital PCR (RT-ddPCR) assay developed absolute quantification influenza A (IAV), B (IBV), syncytial virus (RSV), beta-2-microglobulin transcript as an endogenous internal control (IC B2M). Results first evaluated analytical sensitivity specificity, linearity, reproducibility, recovery rates with excellent performance characteristics then applied to 37 wastewater samples previously commercially available in-house quantitative real-time reverse transcription (RT-qPCR) assays. IAV detected 16/37 (43%), IBV 19/37 (51%), RSV 10/37 (27%) samples. Direct comparison RT-qPCR showed statistically significant high agreement (kappa Cohen’s correlation coefficient: 0.834, p = 0.001) (kappa: 0.773, between two assays, while results 0.355, 0.27) good without statistical significance. Conclusions Overall, ddPCR is cost-effective, specific, can simultaneously detect three common complex matrix Due its resistance inhibitors, could be further used early warning system monitoring.

Language: Английский

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