Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 24, 2023
Abstract
Background.
Anthropogenic
activities
significantly
contribute
to
the
dissemination
of
antibiotic
resistance
genes
(ARGs),
posing
a
substantial
threat
humankind.
The
development
methods
that
allow
robust
ARG
surveillance
is
long-standing
challenge.
Here,
we
use
city-scale
monitoring
ARGs
by
using
two
most
promising
cutting-edge
technologies,
digital
PCR
(dPCR)
and
metagenomics.
Methods.
hot-spots
were
sampled
from
water
wastewater
distribution
systems.
Metagenomics
was
used
provide
broad
view
relative
abundance
richness
in
prokaryotic
viral
fractions.
From
city-core
all
samples,
worldwide
dispersed
sul2
tetW
conferring
sulfonamide
tetracycline,
respectively,
monitored
To
complement,
potential
dispersion
through
airborne
aerosols
addressed.
Results.
largest
overall
detected
hospital
WWTP
input
(up
»6,000
ARGs/Gb
metagenome)
with
large
fraction
unclassified
resistant
bacteria.
DNA
RNA
viruses
up
three
order
magnitude
lower
compared
prokaryotes.
By
metagenomics
dPCR,
similar
tendency
obtained,
higher
abundances
(»125-225
metagenome).
dPCR
absolute
between
6,000-18,600
copies
per
ng
sewage
(»10
5-7
copies/mL)
6.8
copies/mL
seawater
near
discharging
point.
data
suggest
despite
amount
gene
are
discharged
nature,
marine
autochthonous
bacteria
do
not
seem
acquire
these
ARGs.
Airborne
particle
showed
range
potentially
disseminated
at
(outdoor;
32
hits
Gb
m
3
)
followed
indoor
sample.
Conclusions.
more
sensitive
accurate,
while
provided
broader
coverage
detection.
While
desirable,
reliable
correlation
units
into
metagenomic
obtained
here
(r
2
<0.4)
suggesting
methodological
factors
introduce
variability.
Evolutionary
pressure
does
select
targeted
natural
aquatic
environments.
Finally,
non-ventilated
populated
environments
could
represent
human
thread
spreading
should
be
considered
within
One-Health
perspective.
Journal of Hazardous Materials Letters,
Journal Year:
2024,
Volume and Issue:
5, P. 100105 - 100105
Published: March 8, 2024
The
widespread
and
injudicious
usage
of
antibiotics
to
contain
pathogenic
microbial
infections,
coupled
with
inadequate
treatment
wastes
containing
non-metabolized
their
residues
is
resulting
in
rising
environmental
antibiotic
concentrations,
leading
'antibiotic
pollution'.
Antibiotic
pollution
an
emerging
global
challenge
as
it
proving
a
major
driver
for
antibiotic-
or
antimicrobial-resistance
(ABR/
AMR)
escalating
ramifications
worldwide,
precipitating
surge
human
morbidity.
extensive
administration
across
domains
such
healthcare
settings,
agriculture,
aquaculture
farming
endangers
the
presence
diverse
matrices
including
water,
soil,
air.
As
result,
this
dissemination
significantly
leads
development
resistance
profuse
sectors
environment
further
provoking
consequential
health
implications
at
different
trophic
levels.
Owing
its
significance,
mitigate
subsequential
AMR,
governmental
guidelines
regulations
are
implemented
globe
cultivate
public
awareness
concerted
efforts
addressing
predicament.
Given
insufficient
attention
growing
issues,
prompt
must
be
taken
contemplate
current
circumstances
rigor
ongoing
research.
In
review,
we
endeavor
elucidate
escalation
concentration
antibiotic-driven
AMR
air,
soil
environments
potential
threats.
Further,
focuses
on
various
strategies
interventions
attenuate
adverse
impacts
infrastructure,
highlighting
success
stories,
challenges,
future
directions.
Environment International,
Journal Year:
2024,
Volume and Issue:
190, P. 108846 - 108846
Published: June 22, 2024
Natural
environments
play
a
crucial
role
in
transmission
of
antimicrobial
resistance
(AMR).
Development
methods
to
manage
antibiotic
genes
(ARGs)
natural
are
usually
limited
the
laboratory
or
field
scale,
partially
due
complex
dynamics
between
different
environmental
compartments.
Here,
we
conducted
nine-year
longitudinal
profiling
ARGs
at
watershed
and
provide
evidence
that
restrictions
on
livestock
farms
near
water
bodies
significantly
reduced
riverine
ARG
abundance.
Substantial
reductions
were
revealed
relative
abundance
conferring
aminoglycosides
(42%),
MLSB
(36%),
multidrug
(55%),
tetracyclines
(53%),
other
gene
categories
(59%).
Additionally,
improvements
quality
observed,
with
distinct
changes
concentrations
dissolved
reactive
phosphorus,
ammonium,
nitrite,
pH,
oxygen.
Antibiotic
residues
pharmaceuticals
personal
care
products
(PPCPs)
maintain
similarly
low
level.
Microbial
source
tracking
demonstrates
significant
decrease
swine
fecal
indicators,
while
human
pollution
remains
unchanged.
These
results
suggest
reduction
was
substantial
input
resistant
bacteria
from
animal
excreta.
Our
findings
highlight
as
living
for
understanding
AMR,
evaluating
efficacy
regulations,
implications
reducing
risks
associated
AMR
global
scale.
Environmental Microbiology Reports,
Journal Year:
2024,
Volume and Issue:
16(3)
Published: June 1, 2024
Human
activities
are
a
significant
contributor
to
the
spread
of
antibiotic
resistance
genes
(ARGs),
which
pose
serious
threat
human
health.
These
ARGs
can
be
transmitted
through
various
pathways,
including
air,
within
context
One
Health.
This
study
used
metagenomics
monitor
resistomes
in
urban
air
from
two
critical
locations:
wastewater
treatment
plant
and
hospital,
both
indoor
outdoor.
The
presence
cell-like
structures
was
confirmed
fluorescence
microscopy.
metagenomic
analysis
revealed
wide
variety
high
diversity
antibiotic-resistant
bacteria
airborne
particles
collected.
showed
higher
relative
abundances
with
32
ARG
hits
per
Gb
m
