Efficacy of cleaning, disinfection and sterilization modalities for addressing infectious drug-resistant fungi: A review

Journal of Applied Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract This is a timely and important review that focuses on the appropriateness of established cleaning, disinfection sterilization methods to safely effectively address infectious fungal drug-resistant pathogens can potentially contaminate reusable medical devices used in healthcare environment order mitigate risk patient infection. The release World Health Organisation (WHO) priority pathogen list (FPPL) 2022 highlighted public health crisis antimicrobial resistance (AMR) clinically relevant species. Contamination with (including those FPPL) are rare events more likely occur due cross-transmission arising from lapses hand-hygiene practices. Established decontaminate single use devices; however, there assumptions destined for semi-critical appropriately cleaned do not harbour biofilms may undermine ability decontamination these type healthcare. International standards dictate manufacturer's instructions must provide appropriate guidance facilities meet safe reprocessing expectations includes addressing drug resistant pathogens. Increased environmental monitoring vigilance surrounding advised including adherence hand hygiene/aseptic practices cleaning encompassing simplification device features ‘ease-of-reach’. There need promote integrated multi-actor hub approach sophisticated challenges future artificial intelligence machine learning improved diagnostics, monitoring/surveillance (such as wastewater-based epidemiology), sterility assurance design. knowledge gap occurrence potential persistence harboured biofilms, particularly ascertaining efficacy high-level devices.

Language: Английский

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Wastewater based surveillance can be used to reduce clinical testing intensity on a university campus

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 918, P. 170452 - 170452

Published: Jan. 29, 2024

Language: Английский

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Prospective study of Candida auris nucleic acids in wastewater solids in 190 wastewater treatment plants in the United States suggests widespread occurrence

mBio, Journal Year: 2024, Volume and Issue: 15(8)

Published: July 23, 2024

is an emerging, multidrug-resistant fungal pathogen that poses a significant public health threat in healthcare settings. Despite yearly clinical cases rapidly increasing from 77 to 8,131 the last decade, surveillance data on its distribution and prevalence remain limited. We implemented novel assay for

Language: Английский

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Is Candida auris the first multidrug-resistant fungal zoonosis emerging from climate change?

mBio, Journal Year: 2024, Volume and Issue: 15(4)

Published: March 17, 2024

ABSTRACT The emergence and evolutionary path of Candida auris poses an intriguing scientific enigma. Its isolation from a pet dog’s oral cavity in Kansas, reported by White et al. (T. C. White, B. D. Esquivel, E. M. Rouse Salcido, A. Schweiker, al., mBio 15:e03080-23, 2024, https://doi.org/10.1128/mbio.03080-23 ), carries significant implications. This discovery intensifies concerns about its hypothetical capacity for zoonotic transmission, particularly considering the extensive human contact absence secondary animal/human cases both animals humans. findings challenge established notions transmissibility underscore need further investigation into transmission dynamics, especially zooanthroponotic pathways. It raises adaptability different hosts environments, highlighting potential role environmental animal reservoirs dissemination. Critical points include evolving thermal tolerance genetic divergence isolate. case exemplifies necessity integrated One Health approach, combining human, animal, health perspectives, to unravel complexities ’s behavior.

Language: Английский

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Novel Workflows for Separate Isolation of Pathogen RNA or DNA from Wastewater: Detection by Innovative and Conventional qPCR

BIO-PROTOCOL, Journal Year: 2025, Volume and Issue: 15(1365)

Published: Jan. 1, 2025

Wastewater-based surveillance (WBS) can provide a wealth of information regarding the health status communities from measurements nucleic acids found in wastewater. Processing workflows for WBS typically include sample collection, primary concentration step, and lysis microbes to release acids, followed by acid purification molecular-based quantification. This manuscript provides beginning end with an emphasis on filtration-based approaches coupled specific extraction processes. Here, two processing are presented, one focusing RNA-specific pathogens other focused DNA-specific within wastewater: 1) The approach, employed analyzing RNA viruses like severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) couples electronegative filtration wastewater placement filter buffer direct extraction. 2) DNA Candida auris, uses size selection membranes during filtration, subsequently buffer, bead-beating, Separate versus isolations have advantage improving detection target pathogen. A novel aspect workflow is lysates, which shows enhanced recoveries, whereas approach requires bead beating prior Novelty also provided new qPCR called Volcano 2nd Generation (V2G), polymerase capable using as template, bypassing reverse transcriptase step normally required qPCR. Key features • Membrane concentrating suspended solids After concentration, optimized separate recovery DNA. Unique utilized perform analysis, foregoing transcription, RNA. Sample products use molecular techniques (e.g., sequencing) generate high-quality, concentrated extracts minimal inhibitors. Validated through COVID-19 where >1,000 samples >3,000 concentrates produced these been created analyzed, published results. complete protocol was used in: J Biomol Tech (2023), DOI: 10.7171/3fc1f5fe.dfa8d906.

Language: Английский

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A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 238 - 238

Published: Jan. 22, 2025

Geographical variations in infectious diseases create differences public health priorities between high- and low-income countries. Low- middle-income countries (LMICs) face resource constraints that limit adherence to international monitoring standards for wastewater-based epidemiology (WBE). The development of low-cost WBE programs, such as those detect SARS-CoV-2, offers LMICs a promising tool pathogens local concern. In this work, we summarize important wastewater biomarkers their associated challenges, ranging from causing gastroenteritis putative markers plant linked food safety, well antimicrobial resistance. We raise awareness the great potential highlight critical markers, research needs, strategies necessary establish tailored surveillance programs.

Language: Английский

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Culture-Dependent and -Independent Wastewater Surveillance for Multiple Pathogenic Yeasts

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(2), P. 86 - 86

Published: Jan. 23, 2025

Wastewater surveillance is a promising tool to monitor potential outbreaks and determine the disease burden within community. This system has been extensively used polio COVID-19 infection levels, yet few attempts have made apply it monitoring pathogenic yeast. study aimed investigate application of wastewater for potentially yeast in treatment plant influent. was done by comparing culture-dependent data with culture-independent investigating fluconazole concentration wastewater. Additional studies on growth isolated strains were conducted. We found that multiplex PCR detect multiple yeasts holds promise as molecular detection surveillance. Culture-dependent results indicated Candida spp. specifically C. krusei glabrata, most prominent. Growth supported these species grow well this environment while less frequently grew poorly. The from independent techniques showed some correlation, similar being identified both, further promoting use tools highlights presence wastewater, which may indicate prevalence or also be source persons encountering due poor management.

Language: Английский

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The impact of climate change on the epidemiology of fungal infections: implications for diagnosis, treatment, and public health strategies

Therapeutic Advances in Infectious Disease, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 1, 2025

Anthropogenic climate change, primarily driven by greenhouse gas emissions, is reshaping ecosystems and creating conditions that affect 58% of all known human infectious diseases, including fungal infections. Specifically, increasing temperatures, changing precipitation patterns, extreme weather events are influencing growth, distribution, virulence. These factors may expand the geographic range pathogenic fungi, exposing populations to novel, potentially more virulent, or drug-resistant strains. Simultaneously, such as declining immunity, aging populations, increased use immunosuppressive therapies enhancing host susceptibility. This review explores intricate relationship between change infections, highlighting pathogens demonstrate virulence antifungal resistance, along with emerging novel pathogens. The clinical implications profound, morbidity, mortality, spread infections into new regions. Immediate action required develop policies, educational initiatives, therapies, enhance early diagnostic capabilities, address healthcare disparities mitigate growing burden

Language: Английский

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Investigation of the presence of Candida auris and other Candida species in marine waters

International Journal of Environmental Health Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 8

Published: March 28, 2025

Marine recreational waters harbor various microorganisms that may pose health risks to users. However, fungal contamination, particularly by Candida species, has received limited attention despite evidence of their presence and potential concerns. This study aimed assess the microbial quality water at five popular beaches along Persian Gulf, with a specific focus on species. Fecal indicator bacteria (FIB), including Escherichia coli Enterococci, were enumerated in marine samples. Additionally, auris other clinically relevant spp. as well Meyerozyma guilliermondii was investigated using nested, multiplex, conventional PCR assays, respectively. FIB highly prevalent across most sampling locations. C. albicans, tropicalis, glabrata, krusei, M. detected few albicans specifically found locations high concentrations, suggesting an association anthropogenic activities. not this study. The suggests public risk. These findings highlight importance further monitoring for contamination protect guide future management policies.

Language: Английский

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Fluorescence Damping as Primary Interference Mechanism of Humic Acids on qPCR Quantification of SARS-CoV-2 in Wastewater Surveillance

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 492, P. 138283 - 138283

Published: April 17, 2025

Language: Английский

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