Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 17, 2024

We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A B viruses, syncytial virus, human metapneumovirus, enterovirus D68, parainfluenza types 1, 2, 3, 4a, 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis adenovirus, mpox H5 pepper mild mottle virus nucleic acids wastewater solids prospectively at 191 treatment plants 40 states across the United States plus Washington DC. Measurements were made two to seven times per week from 1 January 2022 30 June 2024, depending on plant staff availability. using droplet digital (reverse-transcription-) polymerase chain reaction (ddRT-PCR) following best practices for making environmental molecular biology measurements. These data can be used better understand disease occurrence communities contributing wastewater.

Language: Английский

---

Novel Workflows for Separate Isolation of Pathogen RNA or DNA from Wastewater: Detection by Innovative and Conventional qPCR

BIO-PROTOCOL, Journal Year: 2025, Volume and Issue: 15(1365)

Published: Jan. 1, 2025

Wastewater-based surveillance (WBS) can provide a wealth of information regarding the health status communities from measurements nucleic acids found in wastewater. Processing workflows for WBS typically include sample collection, primary concentration step, and lysis microbes to release acids, followed by acid purification molecular-based quantification. This manuscript provides beginning end with an emphasis on filtration-based approaches coupled specific extraction processes. Here, two processing are presented, one focusing RNA-specific pathogens other focused DNA-specific within wastewater: 1) The approach, employed analyzing RNA viruses like severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) couples electronegative filtration wastewater placement filter buffer direct extraction. 2) DNA Candida auris, uses size selection membranes during filtration, subsequently buffer, bead-beating, Separate versus isolations have advantage improving detection target pathogen. A novel aspect workflow is lysates, which shows enhanced recoveries, whereas approach requires bead beating prior Novelty also provided new qPCR called Volcano 2nd Generation (V2G), polymerase capable using as template, bypassing reverse transcriptase step normally required qPCR. Key features • Membrane concentrating suspended solids After concentration, optimized separate recovery DNA. Unique utilized perform analysis, foregoing transcription, RNA. Sample products use molecular techniques (e.g., sequencing) generate high-quality, concentrated extracts minimal inhibitors. Validated through COVID-19 where >1,000 samples >3,000 concentrates produced these been created analyzed, published results. complete protocol was used in: J Biomol Tech (2023), DOI: 10.7171/3fc1f5fe.dfa8d906.

Language: Английский

---

Culture-Dependent and -Independent Wastewater Surveillance for Multiple Pathogenic Yeasts

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(2), P. 86 - 86

Published: Jan. 23, 2025

Wastewater surveillance is a promising tool to monitor potential outbreaks and determine the disease burden within community. This system has been extensively used polio COVID-19 infection levels, yet few attempts have made apply it monitoring pathogenic yeast. study aimed investigate application of wastewater for potentially yeast in treatment plant influent. was done by comparing culture-dependent data with culture-independent investigating fluconazole concentration wastewater. Additional studies on growth isolated strains were conducted. We found that multiplex PCR detect multiple yeasts holds promise as molecular detection surveillance. Culture-dependent results indicated Candida spp. specifically C. krusei glabrata, most prominent. Growth supported these species grow well this environment while less frequently grew poorly. The from independent techniques showed some correlation, similar being identified both, further promoting use tools highlights presence wastewater, which may indicate prevalence or also be source persons encountering due poor management.

Language: Английский

---

SARS-CoV-2 Surveillance in US Wastewater: Leading Indicators and Data Variability Analysis in the 2023-2024 Season

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

Abstract Wastewater-Based Epidemiology (WBE) has become a powerful tool for assessing disease occurrence in communities. This study investigates the coronavirus 2019 (COVID-19) epidemic United States during 2023-2024 season using wastewater data from 189 treatment plants 40 states and District of Columbia. Severe acute respiratory syndrome 2 (SARS-CoV-2) pepper-mild mottle virus normalized SARS-CoV-2 concentration were compared with COVID-19 hospitalization admission at both national state levels. We further investigate temporal features viral abundance, peak timing cross-correlation lag analyses indicating that concentrations precede admissions by to 12 days. Lastly, we demonstrate plant size, assessed number population served, significant effect on variability measured concentrations. highlights effectiveness WBE as non-invasive, timely resource-efficient monitoring strategy, especially context declining clinical reporting.

Language: Английский

---

SARS-CoV-2 surveillance in US wastewater: Leading indicators and data variability analysis in 2023–2024

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(11), P. e0313927 - e0313927

Published: Nov. 18, 2024

Wastewater-Based Epidemiology (WBE) has become a powerful tool for assessing disease occurrence in communities. This study investigates the coronavirus 2019 (COVID-19) epidemic United States during 2023–2024 using wastewater data from 189 treatment plants 40 states and District of Columbia. Severe acute respiratory syndrome 2 (SARS-CoV-2) pepper-mild mottle virus normalized SARS-CoV-2 RNA concentration were compared with COVID-19 hospitalization admission at both national state levels. We further investigate temporal features viral abundance, peak timing cross-correlation lag analyses indicating that concentrations precede admissions by to 12 days. Lastly, we demonstrate plant size significant effect on variability measured concentrations. highlights effectiveness WBE as non-invasive, timely resource-efficient monitoring strategy, especially context declining clinical reporting.

Language: Английский

---

Wastewater surveillance of Candida auris in Baltimore

International Journal of Hygiene and Environmental Health, Journal Year: 2024, Volume and Issue: 263, P. 114486 - 114486

Published: Nov. 20, 2024

Language: Английский

---