Identification of environmental and methodological factors driving variability of Pepper Mild Mottle Virus (PMMoV) across three wastewater treatment plants in the City of Toronto

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 932, P. 172917 - 172917

Published: May 1, 2024

PMMoV has been widely used to normalize the concentration of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA, influenza, and syncytial virus (RSV) account for variations in fecal content wastewater. is also as an internal RNA recovery control wastewater-based epidemiology (WBE) tests. While potentially useful interpretation WBE data, previous studies have suggested that can be affected by various physico-chemical characteristics There possibility laboratory methods, particularly variability centrifugation steps remove supernatant from pellets cause variability. The goal this study improve our understanding main drivers assessing relationship between concentration, wastewater, methodological approach concentrating wastewater samples. We analyzed 24-hour composite samples collected influent stream three treatment plants (WWTPs) located City Toronto, Ontario, Canada. Samples were 3 5 times per week starting beginning March 2021 mid-July 2023. flow rate was partition data into wet dry weather conditions. Physico-chemical (e.g., total suspended solids (TSS), biological oxygen demand (BOD), alkalinity, electrical conductivity (EC), ammonia (NH

Language: Английский

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Seasonality of enteric viruses and correlation of hepatitis a virus in wastewater with clinical cases

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 967, P. 178862 - 178862

Published: Feb. 16, 2025

Language: Английский

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Longitudinal fecal shedding of SARS-CoV-2, pepper mild mottle virus, and human mitochondrial DNA in COVID-19 patients

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Sept. 11, 2024

Since the coronavirus disease 2019 (COVID-19) pandemic, wastewater-based epidemiology (WBE) has been widely applied in many countries and regions for monitoring COVID-19 transmission population through testing severe acute respiratory syndrome 2 (SARS-CoV-2) wastewater. However, amount of virus shed by individuals over time based on stage infection accurate number infections community creates challenges predicting prevalence interpreting WBE results. In this study, we measured SARS-CoV-2, pepper mild mottle (PMMoV), human mitochondrial DNA (mtDNA) longitudinal fecal samples collected from 42 patients up to days after diagnosis. SARS-CoV-2 RNA was detected 73.1% (19/26) inpatient study participants at least one specimens during sampling period. Most within 3 weeks diagnosis, but five still between 20 60 The median concentration positive 1.08 × 10

Language: Английский

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Alterations of the gut microbiome in HIV infection highlight human anelloviruses as potential predictors of immune recovery

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Oct. 17, 2024

HIV-1 infection is characterized by a massive depletion of mucosal CD4 T cells that triggers cascade events ultimately linking gut microbial dysbiosis to disease progression and pathogenesis. The association between HIV the enteric virome composition less characterized, although viruses are an essential component ecosystem. Here, we performed cross-sectional analysis fecal viral (eukaryotic bacteriophages) bacterial microbiome in people with (PWH) HIV-negative individuals. To gain further insight into composition, HIV-associated immunodeficiency, immune recovery, carried out longitudinal study including 14 PWH who initiated antiretroviral therapy (ART) were followed for 24 months samplings at baseline (before ART) 2, 6, 12, post-ART initiation.

Language: Английский

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Longitudinal Fecal Shedding of SARS-CoV-2, Pepper Mild Mottle Virus, and Human Mitochondrial DNA in COVID-19 Patients

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 23, 2024

Since the coronavirus disease 2019 (COVID-19) pandemic, wastewater-based epidemiology (WBE) has been widely applied in many countries and regions for monitoring COVID-19 transmission population through testing severe acute respiratory syndrome 2 (SARS-CoV-2) wastewater. However, lack of dynamic level viral shedding wastewater accurate number infections community creates challenges predicting prevalence interpreting WBE results. In this study, we measured SARS-CoV-2, pepper mild mottle virus (PMMoV), human mitochondrial DNA (mtDNA) longitudinal fecal samples collected from 42 patients up to days after diagnosis. SARS-CoV-2 RNA was detected 73.1% (19/26) inpatient study participants at least one specimens during sampling period. Most shed within three weeks diagnosis, but five still between 20 60 The median concentration positive 1.08x105 genome copies (GC)/gram dry material. PMMoV mtDNA were 99.4% (154/155) 100% (155/155) all samples, respectively. concentrations 1.73x107 2.49x108 GC/dry gram, These results provide important information about dynamics two indicators patients. showed higher rates, concentrations, less variability individuals than PMMoV, suggesting that could be a better normalization factor PMMoV.

Language: Английский

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Spatiotemporal Variability of the Pepper Mild Mottle Virus Biomarker in Wastewater

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 5(1), P. 341 - 350

Published: Dec. 16, 2024

Since the start of coronavirus-19 pandemic, use wastewater-based epidemiology (WBE) for disease surveillance has increased throughout world. Because wastewater measurements are affected by external factors, processing WBE data typically includes a normalization step in order to adjust (e.g., viral ribonucleic acid (RNA) concentrations) account variation due dynamic population changes, sewer travel effects, or laboratory methods. Pepper mild mottle virus (PMMoV), plant RNA abundant human feces and wastewater, been used as fecal contamination indicator normalize extensively. However, there little work characterize spatiotemporal variability PMMoV which may influence effectiveness adjusting normalizing measurements. Here, we investigate its across space time using collected over two-year period from sewage treatment plants United States. We find that most can be attributed longitude latitude followed site-specific variables. Further research into cross-geographical -temporal comparability PMMoV-normalized pathogen concentrations would strengthen utility WBE.

Language: Английский

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Wastewater monitoring - passive sampling for the detection of SARS-CoV-2 and antibiotic resistance genes in wastewater

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 959, P. 178244 - 178244

Published: Dec. 26, 2024

Language: Английский

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